Efficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells

Rong Chen*, Hui Yu*, Zhen-Yu Jia, Qun-Li Yao, Gao-Jun TengJiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, People&rsquo;s Republic of China *These authors have contributed equally t...

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Autores principales:	Zhen-Yu Jia, Hui Yu, Rong Chen, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/325e0331f1a84f1f8e8caedcc2e5fcaf
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spelling	oai:doaj.org-article:325e0331f1a84f1f8e8caedcc2e5fcaf2021-12-02T02:36:42ZEfficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells1176-91141178-2013https://doaj.org/article/325e0331f1a84f1f8e8caedcc2e5fcaf2011-03-01T00:00:00Zhttp://www.dovepress.com/efficient-nano-iron-particle-labeling-and-noninvasive-mr-imaging-of-mo-a6643https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Rong Chen, Hui Yu, Zhen-Yu Jia, Qun-Li Yao, Gao-Jun TengJiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, People&rsquo;s Republic of China These authors have contributed equally to this workAbstract: In this study, we sought to label mouse bone marrow-derived endothelial progenitor cells (EPCs) with Resovist&reg; in vitro and to image them using 7.0 Tesla (T) magnetic resonance imaging (MRI). Mouse bone marrow-derived EPCs were cultured in endothelial basal medium with endothelial growth supplement. They were then characterized by immunocytochemistry, flow cytometry, and fluorescence quantitative polymerase chain reaction. Their functions were evaluated by measuring their uptake of 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-labeled acetylated low-density lipoprotein (Dil-Ac-LDL), binding of fluorine isothiocyanate (FITC)-labeled Ulex europaeus agglutinin (UEA), and formation of capillary-like networks. EPCs were labeled with superparamagnetic iron oxide (SPIO) and their proliferation was then assessed in a water-soluble tetrazolium (WST-8)-based cell proliferation assay. Spin echo sequence (multislice, multiecho [MSME]) and gradient echo sequence (2D-FLASH) were used to detect differences in the numbers of labeled cells by 7.0 T MRI. The results showed that the cultured cells were of &ldquo;cobblestone&rdquo;-like shape and positive for CD133, CD34, CD31, von Willebrand factor, kinase domain receptor, and CD45, but negative for F4/80. They could take up Dil-Ac-LDL, bind FITC-UEA, and form capillary-like networks on Matrigel in vitro. Prussian-blue staining demonstrated that the cells were efficiently labeled with SPIO. The single-cell T2 effect was more obvious in the 2D-FLASH sequence than in the MSME sequence. Further, there were almost no adverse effects on cell vitality and proliferation. In conclusion, mouse bone marrow-derived EPCs can be efficiently labeled with SPIO and imaged with 7.0-T MRI. They may thus be traced by MRI following transplantation for blood vessel disorders and cancer treatment.Keywords: endothelial progenitor cells, cell labeling, Resovist, magnetic resonance imaging Zhen-Yu JiaHui YuRong Chenet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 511-519 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhen-Yu Jia Hui Yu Rong Chen et al Efficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells
description	Rong Chen, Hui Yu, Zhen-Yu Jia, Qun-Li Yao, Gao-Jun TengJiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, People&rsquo;s Republic of China These authors have contributed equally to this workAbstract: In this study, we sought to label mouse bone marrow-derived endothelial progenitor cells (EPCs) with Resovist&reg; in vitro and to image them using 7.0 Tesla (T) magnetic resonance imaging (MRI). Mouse bone marrow-derived EPCs were cultured in endothelial basal medium with endothelial growth supplement. They were then characterized by immunocytochemistry, flow cytometry, and fluorescence quantitative polymerase chain reaction. Their functions were evaluated by measuring their uptake of 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-labeled acetylated low-density lipoprotein (Dil-Ac-LDL), binding of fluorine isothiocyanate (FITC)-labeled Ulex europaeus agglutinin (UEA), and formation of capillary-like networks. EPCs were labeled with superparamagnetic iron oxide (SPIO) and their proliferation was then assessed in a water-soluble tetrazolium (WST-8)-based cell proliferation assay. Spin echo sequence (multislice, multiecho [MSME]) and gradient echo sequence (2D-FLASH) were used to detect differences in the numbers of labeled cells by 7.0 T MRI. The results showed that the cultured cells were of &ldquo;cobblestone&rdquo;-like shape and positive for CD133, CD34, CD31, von Willebrand factor, kinase domain receptor, and CD45, but negative for F4/80. They could take up Dil-Ac-LDL, bind FITC-UEA, and form capillary-like networks on Matrigel in vitro. Prussian-blue staining demonstrated that the cells were efficiently labeled with SPIO. The single-cell T2 effect was more obvious in the 2D-FLASH sequence than in the MSME sequence. Further, there were almost no adverse effects on cell vitality and proliferation. In conclusion, mouse bone marrow-derived EPCs can be efficiently labeled with SPIO and imaged with 7.0-T MRI. They may thus be traced by MRI following transplantation for blood vessel disorders and cancer treatment.Keywords: endothelial progenitor cells, cell labeling, Resovist, magnetic resonance imaging
format	article
author	Zhen-Yu Jia Hui Yu Rong Chen et al
author_facet	Zhen-Yu Jia Hui Yu Rong Chen et al
author_sort	Zhen-Yu Jia
title	Efficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells
title_short	Efficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells
title_full	Efficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells
title_fullStr	Efficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells
title_full_unstemmed	Efficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells
title_sort	efficient nano iron particle-labeling and noninvasive mr imaging of mouse bone marrow-derived endothelial progenitor cells
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/325e0331f1a84f1f8e8caedcc2e5fcaf
work_keys_str_mv	AT zhenyujia efficientnanoironparticlelabelingandnoninvasivemrimagingofmousebonemarrowderivedendothelialprogenitorcells AT huiyu efficientnanoironparticlelabelingandnoninvasivemrimagingofmousebonemarrowderivedendothelialprogenitorcells AT rongchen efficientnanoironparticlelabelingandnoninvasivemrimagingofmousebonemarrowderivedendothelialprogenitorcells AT etal efficientnanoironparticlelabelingandnoninvasivemrimagingofmousebonemarrowderivedendothelialprogenitorcells
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Efficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells

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