Species and Genetic Diversity of Representatives of the Anaplasmataceae Family Found in the Sympatry Zone of the Ixodes, Dermacentor and Haemaphysalis Genera Ticks

Introduction.On the territory of the Ekhirit-Bulagatsky district of the Irkutsk region zones of sympatry of four Ixodes ticks species are found, where the species and genetic diversity of infectious agents transmitted through tick bites may be more pronounced than in foci with a mono-dominant type o...

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Autores principales:	E. K. Doroshchenko, O. V. Lisak, V. A. Rar, O. V. Suntsova, Yu. S. Savinova, I. V. Kozlova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2019
Materias:	anaplasma ehrlichia ixodic ticks reservoir hosts polymerase chain reaction Science Q
Acceso en línea:	https://doaj.org/article/3260b11bfe02471d935274023217902e
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spelling	oai:doaj.org-article:3260b11bfe02471d935274023217902e2021-11-23T06:14:42ZSpecies and Genetic Diversity of Representatives of the <i>Anaplasmataceae</i> Family Found in the Sympatry Zone of the <i>Ixodes, Dermacentor</i> and <i>Haemaphysalis</i> Genera Ticks2541-94202587-959610.29413/ABS.2019-4.2.18https://doaj.org/article/3260b11bfe02471d935274023217902e2019-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2056https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Introduction.On the territory of the Ekhirit-Bulagatsky district of the Irkutsk region zones of sympatry of four Ixodes ticks species are found, where the species and genetic diversity of infectious agents transmitted through tick bites may be more pronounced than in foci with a mono-dominant type of ticks’ population. In this connection, the study of the species and genetic diversity of representatives of the Anaplasmataceae family in the sympatry zone of the Ixodes ticks of closely related species was of scientific interest.Objective: To study the species and genetic diversity of members of the Anaplasmataceae family in the zones of sympatry of Ixodes ticks Ixodes persulcatus, Dermacentor silvarum, D. nuttalli and Haemaphysalis concinna, to identify the main carriers and potential reservoir hosts of ehrlichia and anaplasma.Methods. In the course of the study, 1106 specimens of adult ticks and 49 samples of small mammalian livers from the Ekhirit-Bulagatsky area were analyzed. Anaplasma and ehrlichia DNA were detected by two-round PCR in the presence of genus- and species-specific primers from the 16S rRNA gene region. The nucleotide sequences of the 16S rRNA gene and the fragment of the groESL operon were identified in some samples. Sequencing was carried out according to the Sanger method. Comparative analysis was performed using the BLASTN program and ClustalW method. Epidemiological data analysis was performed using parametric methods of statistical processing of the material.Results. The DNA of Ehrlichia muris and Anaplasma phagocytophilum were detected in all studied species of ticks in their sympatry area. However, the rate of infection of taiga ticks was significantly higher than that of H. concinna and Dermacentor spp. Potential reservoir hosts of the Anaplasmataceae family members can be classified as Microtus oeconomus, M. gregalis, Myodes rutilus and Sorex spp. When analyzing the nucleotide sequences of the 16S rRNA gene, three genetic variants of anaplasma were detected. The nucleotide sequences of the A. phagocytophilum groESL operon belonged to two genetic groups.E. K. DoroshchenkoO. V. LisakV. A. RarO. V. SuntsovaYu. S. SavinovaI. V. KozlovaScientific Сentre for Family Health and Human Reproduction Problemsarticleanaplasmaehrlichiaixodic ticksreservoir hostspolymerase chain reactionScienceQRUActa Biomedica Scientifica, Vol 4, Iss 2, Pp 127-135 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	anaplasma ehrlichia ixodic ticks reservoir hosts polymerase chain reaction Science Q
spellingShingle	anaplasma ehrlichia ixodic ticks reservoir hosts polymerase chain reaction Science Q E. K. Doroshchenko O. V. Lisak V. A. Rar O. V. Suntsova Yu. S. Savinova I. V. Kozlova Species and Genetic Diversity of Representatives of the <i>Anaplasmataceae</i> Family Found in the Sympatry Zone of the <i>Ixodes, Dermacentor</i> and <i>Haemaphysalis</i> Genera Ticks
description	Introduction.On the territory of the Ekhirit-Bulagatsky district of the Irkutsk region zones of sympatry of four Ixodes ticks species are found, where the species and genetic diversity of infectious agents transmitted through tick bites may be more pronounced than in foci with a mono-dominant type of ticks’ population. In this connection, the study of the species and genetic diversity of representatives of the Anaplasmataceae family in the sympatry zone of the Ixodes ticks of closely related species was of scientific interest.Objective: To study the species and genetic diversity of members of the Anaplasmataceae family in the zones of sympatry of Ixodes ticks Ixodes persulcatus, Dermacentor silvarum, D. nuttalli and Haemaphysalis concinna, to identify the main carriers and potential reservoir hosts of ehrlichia and anaplasma.Methods. In the course of the study, 1106 specimens of adult ticks and 49 samples of small mammalian livers from the Ekhirit-Bulagatsky area were analyzed. Anaplasma and ehrlichia DNA were detected by two-round PCR in the presence of genus- and species-specific primers from the 16S rRNA gene region. The nucleotide sequences of the 16S rRNA gene and the fragment of the groESL operon were identified in some samples. Sequencing was carried out according to the Sanger method. Comparative analysis was performed using the BLASTN program and ClustalW method. Epidemiological data analysis was performed using parametric methods of statistical processing of the material.Results. The DNA of Ehrlichia muris and Anaplasma phagocytophilum were detected in all studied species of ticks in their sympatry area. However, the rate of infection of taiga ticks was significantly higher than that of H. concinna and Dermacentor spp. Potential reservoir hosts of the Anaplasmataceae family members can be classified as Microtus oeconomus, M. gregalis, Myodes rutilus and Sorex spp. When analyzing the nucleotide sequences of the 16S rRNA gene, three genetic variants of anaplasma were detected. The nucleotide sequences of the A. phagocytophilum groESL operon belonged to two genetic groups.
format	article
author	E. K. Doroshchenko O. V. Lisak V. A. Rar O. V. Suntsova Yu. S. Savinova I. V. Kozlova
author_facet	E. K. Doroshchenko O. V. Lisak V. A. Rar O. V. Suntsova Yu. S. Savinova I. V. Kozlova
author_sort	E. K. Doroshchenko
title	Species and Genetic Diversity of Representatives of the <i>Anaplasmataceae</i> Family Found in the Sympatry Zone of the <i>Ixodes, Dermacentor</i> and <i>Haemaphysalis</i> Genera Ticks
title_short	Species and Genetic Diversity of Representatives of the <i>Anaplasmataceae</i> Family Found in the Sympatry Zone of the <i>Ixodes, Dermacentor</i> and <i>Haemaphysalis</i> Genera Ticks
title_full	Species and Genetic Diversity of Representatives of the <i>Anaplasmataceae</i> Family Found in the Sympatry Zone of the <i>Ixodes, Dermacentor</i> and <i>Haemaphysalis</i> Genera Ticks
title_fullStr	Species and Genetic Diversity of Representatives of the <i>Anaplasmataceae</i> Family Found in the Sympatry Zone of the <i>Ixodes, Dermacentor</i> and <i>Haemaphysalis</i> Genera Ticks
title_full_unstemmed	Species and Genetic Diversity of Representatives of the <i>Anaplasmataceae</i> Family Found in the Sympatry Zone of the <i>Ixodes, Dermacentor</i> and <i>Haemaphysalis</i> Genera Ticks
title_sort	species and genetic diversity of representatives of the <i>anaplasmataceae</i> family found in the sympatry zone of the <i>ixodes, dermacentor</i> and <i>haemaphysalis</i> genera ticks
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2019
url	https://doaj.org/article/3260b11bfe02471d935274023217902e
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Species and Genetic Diversity of Representatives of the <i>Anaplasmataceae</i> Family Found in the Sympatry Zone of the <i>Ixodes, Dermacentor</i> and <i>Haemaphysalis</i> Genera Ticks

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