Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle

Self-assembling proteins have important applications in industrial technologies, but it is difficult to predict how mutations affect assembly. Here the authors present SyMAPS, coupling comprehensive codon mutagenesis with high-throughput sequencing, and apply it to bacteriophage capsid protein.

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Autores principales:	Emily C. Hartman, Christopher M. Jakobson, Andrew H. Favor, Marco J. Lobba, Ester Álvarez-Benedicto, Matthew B. Francis, Danielle Tullman-Ercek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/32650acf858e4272b1227a70f3e4f8ee
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spelling	oai:doaj.org-article:32650acf858e4272b1227a70f3e4f8ee2021-12-02T16:49:56ZQuantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle10.1038/s41467-018-03783-y2041-1723https://doaj.org/article/32650acf858e4272b1227a70f3e4f8ee2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03783-yhttps://doaj.org/toc/2041-1723Self-assembling proteins have important applications in industrial technologies, but it is difficult to predict how mutations affect assembly. Here the authors present SyMAPS, coupling comprehensive codon mutagenesis with high-throughput sequencing, and apply it to bacteriophage capsid protein.Emily C. HartmanChristopher M. JakobsonAndrew H. FavorMarco J. LobbaEster Álvarez-BenedictoMatthew B. FrancisDanielle Tullman-ErcekNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Emily C. Hartman Christopher M. Jakobson Andrew H. Favor Marco J. Lobba Ester Álvarez-Benedicto Matthew B. Francis Danielle Tullman-Ercek Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle
description	Self-assembling proteins have important applications in industrial technologies, but it is difficult to predict how mutations affect assembly. Here the authors present SyMAPS, coupling comprehensive codon mutagenesis with high-throughput sequencing, and apply it to bacteriophage capsid protein.
format	article
author	Emily C. Hartman Christopher M. Jakobson Andrew H. Favor Marco J. Lobba Ester Álvarez-Benedicto Matthew B. Francis Danielle Tullman-Ercek
author_facet	Emily C. Hartman Christopher M. Jakobson Andrew H. Favor Marco J. Lobba Ester Álvarez-Benedicto Matthew B. Francis Danielle Tullman-Ercek
author_sort	Emily C. Hartman
title	Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle
title_short	Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle
title_full	Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle
title_fullStr	Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle
title_full_unstemmed	Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle
title_sort	quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/32650acf858e4272b1227a70f3e4f8ee
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Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle

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