New data on C1qDC from the colonial ascidian Botryllus schlosseri

In the compound ascidian Botryllus schlosseri, we recently identified a novel C1q-domain-containing (C1qDC) protein expressed by circulating immunocytes, called BsC1qDC. It has two globular C1q domains and a signal peptide and can act either as an opsonin and facilitate the phagocytosis of nonself...

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Autores principales:	A Peronato, G Minervini, N Franchi, L Ballarin
Formato:	article
Lenguaje:	EN
Publicado:	University of Modena and Reggio Emilia 2021
Materias:	colonial ascidians Botryllus C1qDC complement Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/326f2bc446f54814a5a701c868fc6d70
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spelling	oai:doaj.org-article:326f2bc446f54814a5a701c868fc6d702021-12-02T19:36:00ZNew data on C1qDC from the colonial ascidian Botryllus schlosseri1824-307Xhttps://doaj.org/article/326f2bc446f54814a5a701c868fc6d702021-10-01T00:00:00Zhttps://www.isj.unimore.it/index.php/ISJ/article/view/697https://doaj.org/toc/1824-307X In the compound ascidian Botryllus schlosseri, we recently identified a novel C1q-domain-containing (C1qDC) protein expressed by circulating immunocytes, called BsC1qDC. It has two globular C1q domains and a signal peptide and can act either as an opsonin and facilitate the phagocytosis of nonself particles or as a cytokine and stimulate the degranulation of cytotoxic cells. In the present work, we used a commercial antibody raised against human CTRP4 (hCTRP4) to provide additional evidences of the involvement of this molecule in immune responses. The antibody was validated in immunoblot analysis and recognizes a band corresponding to the expected molecular weight inferred from the analysis of the amino acid sequence of BsC1qDC. The presence of the antibody in the culture medium in phagocytosis and degranulation assays significantly reduced the two responses. In addition, the relationships between complement C3 activation and bsc1qdc transcription was studied using the injection of C3aR agonist in the colonial vasculature. A PeronatoG MinerviniN FranchiL BallarinUniversity of Modena and Reggio Emiliaarticlecolonial ascidiansBotryllusC1qDCcomplementBiology (General)QH301-705.5ENInvertebrate Survival Journal, Vol 18, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	colonial ascidians Botryllus C1qDC complement Biology (General) QH301-705.5
spellingShingle	colonial ascidians Botryllus C1qDC complement Biology (General) QH301-705.5 A Peronato G Minervini N Franchi L Ballarin New data on C1qDC from the colonial ascidian Botryllus schlosseri
description	In the compound ascidian Botryllus schlosseri, we recently identified a novel C1q-domain-containing (C1qDC) protein expressed by circulating immunocytes, called BsC1qDC. It has two globular C1q domains and a signal peptide and can act either as an opsonin and facilitate the phagocytosis of nonself particles or as a cytokine and stimulate the degranulation of cytotoxic cells. In the present work, we used a commercial antibody raised against human CTRP4 (hCTRP4) to provide additional evidences of the involvement of this molecule in immune responses. The antibody was validated in immunoblot analysis and recognizes a band corresponding to the expected molecular weight inferred from the analysis of the amino acid sequence of BsC1qDC. The presence of the antibody in the culture medium in phagocytosis and degranulation assays significantly reduced the two responses. In addition, the relationships between complement C3 activation and bsc1qdc transcription was studied using the injection of C3aR agonist in the colonial vasculature.
format	article
author	A Peronato G Minervini N Franchi L Ballarin
author_facet	A Peronato G Minervini N Franchi L Ballarin
author_sort	A Peronato
title	New data on C1qDC from the colonial ascidian Botryllus schlosseri
title_short	New data on C1qDC from the colonial ascidian Botryllus schlosseri
title_full	New data on C1qDC from the colonial ascidian Botryllus schlosseri
title_fullStr	New data on C1qDC from the colonial ascidian Botryllus schlosseri
title_full_unstemmed	New data on C1qDC from the colonial ascidian Botryllus schlosseri
title_sort	new data on c1qdc from the colonial ascidian botryllus schlosseri
publisher	University of Modena and Reggio Emilia
publishDate	2021
url	https://doaj.org/article/326f2bc446f54814a5a701c868fc6d70
work_keys_str_mv	AT aperonato newdataonc1qdcfromthecolonialascidianbotryllusschlosseri AT gminervini newdataonc1qdcfromthecolonialascidianbotryllusschlosseri AT nfranchi newdataonc1qdcfromthecolonialascidianbotryllusschlosseri AT lballarin newdataonc1qdcfromthecolonialascidianbotryllusschlosseri
_version_	1718376373629747200

New data on C1qDC from the colonial ascidian Botryllus schlosseri

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