Altered metabolic signature in pre-diabetic NOD mice.

Altered metabolism proceeding seroconversion in children progressing to Type 1 diabetes has previously been demonstrated. We tested the hypothesis that non-obese diabetic (NOD) mice show a similarly altered metabolic profile compared to C57BL/6 mice. Blood samples from NOD and C57BL/6 female mice wa...

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Autores principales:	Rasmus Madsen, Viqar Showkat Banday, Thomas Moritz, Johan Trygg, Kristina Lejon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/32720852024041d8997e596e9ae42772
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spelling	oai:doaj.org-article:32720852024041d8997e596e9ae427722021-11-18T07:22:09ZAltered metabolic signature in pre-diabetic NOD mice.1932-620310.1371/journal.pone.0035445https://doaj.org/article/32720852024041d8997e596e9ae427722012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22514744/?tool=EBIhttps://doaj.org/toc/1932-6203Altered metabolism proceeding seroconversion in children progressing to Type 1 diabetes has previously been demonstrated. We tested the hypothesis that non-obese diabetic (NOD) mice show a similarly altered metabolic profile compared to C57BL/6 mice. Blood samples from NOD and C57BL/6 female mice was collected at 0, 1, 2, 3, 4, 5, 6, 7, 9, 11, 13 and 15 weeks and the metabolite content was analyzed using GC-MS. Based on the data of 89 identified metabolites OPLS-DA analysis was employed to determine the most discriminative metabolites. In silico analysis of potential involved metabolic enzymes was performed using the dbSNP data base. Already at 0 weeks NOD mice displayed a unique metabolic signature compared to C57BL/6. A shift in the metabolism was observed for both strains the first weeks of life, a pattern that stabilized after 5 weeks of age. Multivariate analysis revealed the most discriminative metabolites, which included inosine and glutamic acid. In silico analysis of the genes in the involved metabolic pathways revealed several SNPs in either regulatory or coding regions, some in previously defined insulin dependent diabetes (Idd) regions. Our result shows that NOD mice display an altered metabolic profile that is partly resembling the previously observation made in children progressing to Type 1 diabetes. The level of glutamic acid was one of the most discriminative metabolites in addition to several metabolites in the TCA cycle and nucleic acid components. The in silico analysis indicated that the genes responsible for this reside within previously defined Idd regions.Rasmus MadsenViqar Showkat BandayThomas MoritzJohan TryggKristina LejonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35445 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rasmus Madsen Viqar Showkat Banday Thomas Moritz Johan Trygg Kristina Lejon Altered metabolic signature in pre-diabetic NOD mice.
description	Altered metabolism proceeding seroconversion in children progressing to Type 1 diabetes has previously been demonstrated. We tested the hypothesis that non-obese diabetic (NOD) mice show a similarly altered metabolic profile compared to C57BL/6 mice. Blood samples from NOD and C57BL/6 female mice was collected at 0, 1, 2, 3, 4, 5, 6, 7, 9, 11, 13 and 15 weeks and the metabolite content was analyzed using GC-MS. Based on the data of 89 identified metabolites OPLS-DA analysis was employed to determine the most discriminative metabolites. In silico analysis of potential involved metabolic enzymes was performed using the dbSNP data base. Already at 0 weeks NOD mice displayed a unique metabolic signature compared to C57BL/6. A shift in the metabolism was observed for both strains the first weeks of life, a pattern that stabilized after 5 weeks of age. Multivariate analysis revealed the most discriminative metabolites, which included inosine and glutamic acid. In silico analysis of the genes in the involved metabolic pathways revealed several SNPs in either regulatory or coding regions, some in previously defined insulin dependent diabetes (Idd) regions. Our result shows that NOD mice display an altered metabolic profile that is partly resembling the previously observation made in children progressing to Type 1 diabetes. The level of glutamic acid was one of the most discriminative metabolites in addition to several metabolites in the TCA cycle and nucleic acid components. The in silico analysis indicated that the genes responsible for this reside within previously defined Idd regions.
format	article
author	Rasmus Madsen Viqar Showkat Banday Thomas Moritz Johan Trygg Kristina Lejon
author_facet	Rasmus Madsen Viqar Showkat Banday Thomas Moritz Johan Trygg Kristina Lejon
author_sort	Rasmus Madsen
title	Altered metabolic signature in pre-diabetic NOD mice.
title_short	Altered metabolic signature in pre-diabetic NOD mice.
title_full	Altered metabolic signature in pre-diabetic NOD mice.
title_fullStr	Altered metabolic signature in pre-diabetic NOD mice.
title_full_unstemmed	Altered metabolic signature in pre-diabetic NOD mice.
title_sort	altered metabolic signature in pre-diabetic nod mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/32720852024041d8997e596e9ae42772
work_keys_str_mv	AT rasmusmadsen alteredmetabolicsignatureinprediabeticnodmice AT viqarshowkatbanday alteredmetabolicsignatureinprediabeticnodmice AT thomasmoritz alteredmetabolicsignatureinprediabeticnodmice AT johantrygg alteredmetabolicsignatureinprediabeticnodmice AT kristinalejon alteredmetabolicsignatureinprediabeticnodmice
_version_	1718423527146651648

Altered metabolic signature in pre-diabetic NOD mice.

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