Abundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site

<b>Background:</b> Antibiotics releasing from the manufacturing sites to the surrounding environment has been identified as a risk factor for the development of antibiotic resistance of bacterial pathogens. However, the knowledge of the abundance and distribution of antibiotic resistance...

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Autores principales:	Junjie Miao, Zhendong Yin, Yuqin Yang, Yiwen Liang, Xiangdong Xu, Hongmei Shi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibiotic resistance genes environmental media abundance dynamic distribution qPCR Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/3276205c452a4f78b46c12c1654c5447
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spelling	oai:doaj.org-article:3276205c452a4f78b46c12c1654c54472021-11-25T16:23:38ZAbundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site10.3390/antibiotics101113612079-6382https://doaj.org/article/3276205c452a4f78b46c12c1654c54472021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1361https://doaj.org/toc/2079-6382<b>Background:</b> Antibiotics releasing from the manufacturing sites to the surrounding environment has been identified as a risk factor for the development of antibiotic resistance of bacterial pathogens. However, the knowledge of the abundance and distribution of antibiotic resistance genes (ARGs) influenced by antibiotic pollution is still limited. <b>Methods:</b> In this work, the contamination by resistance genes of the environmental media including an urban river and soil along the river located near the sewage outlet of a veterinary antibiotic manufacturing site in Shijiazhuang, China, was assessed. The abundance and dynamic distribution of ARGs in different sampling points and during different seasons were analyzed using fluorescent quantitative PCR method (qPCR). <b>Results:</b> A total of 11 resistance genes, one integron and one transposon were detected in water and soils around the pharmaceutical factory, and among which, the sulfonamide resistance genes <i>sul1</i> and β-lactam resistance genes <i>blaSHV</i> were the most abundant genes. The relative abundance of ARGs in both river water and soil samples collected at the downstream of the sewage outlet was higher than that of samples collected at the upstream, non-polluted areas (<i>p</i> < 0.05). The mobile genetic elements (MGEs) integron in river was significantly correlated (<i>p</i> < 0.05) with the relative abundance of ARGs. <b>Conclusions:</b> The results indicate that the discharge of waste from antibiotic manufacturing site may pose a risk of horizontal transfer of ARGs.Junjie MiaoZhendong YinYuqin YangYiwen LiangXiangdong XuHongmei ShiMDPI AGarticleantibiotic resistance genesenvironmental mediaabundancedynamic distributionqPCRTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1361, p 1361 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistance genes environmental media abundance dynamic distribution qPCR Therapeutics. Pharmacology RM1-950
spellingShingle	antibiotic resistance genes environmental media abundance dynamic distribution qPCR Therapeutics. Pharmacology RM1-950 Junjie Miao Zhendong Yin Yuqin Yang Yiwen Liang Xiangdong Xu Hongmei Shi Abundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site
description	<b>Background:</b> Antibiotics releasing from the manufacturing sites to the surrounding environment has been identified as a risk factor for the development of antibiotic resistance of bacterial pathogens. However, the knowledge of the abundance and distribution of antibiotic resistance genes (ARGs) influenced by antibiotic pollution is still limited. <b>Methods:</b> In this work, the contamination by resistance genes of the environmental media including an urban river and soil along the river located near the sewage outlet of a veterinary antibiotic manufacturing site in Shijiazhuang, China, was assessed. The abundance and dynamic distribution of ARGs in different sampling points and during different seasons were analyzed using fluorescent quantitative PCR method (qPCR). <b>Results:</b> A total of 11 resistance genes, one integron and one transposon were detected in water and soils around the pharmaceutical factory, and among which, the sulfonamide resistance genes <i>sul1</i> and β-lactam resistance genes <i>blaSHV</i> were the most abundant genes. The relative abundance of ARGs in both river water and soil samples collected at the downstream of the sewage outlet was higher than that of samples collected at the upstream, non-polluted areas (<i>p</i> < 0.05). The mobile genetic elements (MGEs) integron in river was significantly correlated (<i>p</i> < 0.05) with the relative abundance of ARGs. <b>Conclusions:</b> The results indicate that the discharge of waste from antibiotic manufacturing site may pose a risk of horizontal transfer of ARGs.
format	article
author	Junjie Miao Zhendong Yin Yuqin Yang Yiwen Liang Xiangdong Xu Hongmei Shi
author_facet	Junjie Miao Zhendong Yin Yuqin Yang Yiwen Liang Xiangdong Xu Hongmei Shi
author_sort	Junjie Miao
title	Abundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site
title_short	Abundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site
title_full	Abundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site
title_fullStr	Abundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site
title_full_unstemmed	Abundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site
title_sort	abundance and dynamic distribution of antibiotic resistance genes in the environment surrounding a veterinary antibiotic manufacturing site
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3276205c452a4f78b46c12c1654c5447
work_keys_str_mv	AT junjiemiao abundanceanddynamicdistributionofantibioticresistancegenesintheenvironmentsurroundingaveterinaryantibioticmanufacturingsite AT zhendongyin abundanceanddynamicdistributionofantibioticresistancegenesintheenvironmentsurroundingaveterinaryantibioticmanufacturingsite AT yuqinyang abundanceanddynamicdistributionofantibioticresistancegenesintheenvironmentsurroundingaveterinaryantibioticmanufacturingsite AT yiwenliang abundanceanddynamicdistributionofantibioticresistancegenesintheenvironmentsurroundingaveterinaryantibioticmanufacturingsite AT xiangdongxu abundanceanddynamicdistributionofantibioticresistancegenesintheenvironmentsurroundingaveterinaryantibioticmanufacturingsite AT hongmeishi abundanceanddynamicdistributionofantibioticresistancegenesintheenvironmentsurroundingaveterinaryantibioticmanufacturingsite
_version_	1718413191611940864

Abundance and Dynamic Distribution of Antibiotic Resistance Genes in the Environment Surrounding a Veterinary Antibiotic Manufacturing Site

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