In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing

Acute and chronic wounds affect millions of people around the world, imposing a growing financial burden on patients and hospitals. Despite the application of current wound management strategies, the physiological healing process is disrupted in many cases, resulting in impaired wound healing. There...

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Autores principales:	Kristo Nuutila, Mohamadmahdi Samandari, Yori Endo, Yuteng Zhang, Jacob Quint, Tannin A. Schmidt, Ali Tamayol, Indranil Sinha
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	In vivo printing Handheld bioprinter Adhesive scaffolds GelMA VEGF Wound healing Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/32779d4bf58149daa66fc087c6a8e1f1
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id	oai:doaj.org-article:32779d4bf58149daa66fc087c6a8e1f1
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spelling	oai:doaj.org-article:32779d4bf58149daa66fc087c6a8e1f12021-11-04T04:36:06ZIn vivo printing of growth factor-eluting adhesive scaffolds improves wound healing2452-199X10.1016/j.bioactmat.2021.06.030https://doaj.org/article/32779d4bf58149daa66fc087c6a8e1f12022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21003169https://doaj.org/toc/2452-199XAcute and chronic wounds affect millions of people around the world, imposing a growing financial burden on patients and hospitals. Despite the application of current wound management strategies, the physiological healing process is disrupted in many cases, resulting in impaired wound healing. Therefore, more efficient and easy-to-use treatment modalities are needed. In this study, we demonstrate the benefit of in vivo printed, growth factor-eluting adhesive scaffolds for the treatment of full-thickness wounds in a porcine model. A custom-made handheld printer is implemented to finely print gelatin-methacryloyl (GelMA) hydrogel containing vascular endothelial growth factor (VEGF) into the wounds. In vitro and in vivo results show that the in situ GelMA crosslinking induces a strong scaffold adhesion and enables printing on curved surfaces of wet tissues, without the need for any sutures. The scaffold is further shown to offer a sustained release of VEGF, enhancing the migration of endothelial cells in vitro. Histological analyses demonstrate that the administration of the VEGF-eluting GelMA scaffolds that remain adherent to the wound bed significantly improves the quality of healing in porcine wounds. The introduced in vivo printing strategy for wound healing applications is translational and convenient to use in any place, such as an operating room, and does not require expensive bioprinters or imaging modalities.Kristo NuutilaMohamadmahdi SamandariYori EndoYuteng ZhangJacob QuintTannin A. SchmidtAli TamayolIndranil SinhaKeAi Communications Co., Ltd.articleIn vivo printingHandheld bioprinterAdhesive scaffoldsGelMAVEGFWound healingMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 8, Iss , Pp 296-308 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	In vivo printing Handheld bioprinter Adhesive scaffolds GelMA VEGF Wound healing Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	In vivo printing Handheld bioprinter Adhesive scaffolds GelMA VEGF Wound healing Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Kristo Nuutila Mohamadmahdi Samandari Yori Endo Yuteng Zhang Jacob Quint Tannin A. Schmidt Ali Tamayol Indranil Sinha In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing
description	Acute and chronic wounds affect millions of people around the world, imposing a growing financial burden on patients and hospitals. Despite the application of current wound management strategies, the physiological healing process is disrupted in many cases, resulting in impaired wound healing. Therefore, more efficient and easy-to-use treatment modalities are needed. In this study, we demonstrate the benefit of in vivo printed, growth factor-eluting adhesive scaffolds for the treatment of full-thickness wounds in a porcine model. A custom-made handheld printer is implemented to finely print gelatin-methacryloyl (GelMA) hydrogel containing vascular endothelial growth factor (VEGF) into the wounds. In vitro and in vivo results show that the in situ GelMA crosslinking induces a strong scaffold adhesion and enables printing on curved surfaces of wet tissues, without the need for any sutures. The scaffold is further shown to offer a sustained release of VEGF, enhancing the migration of endothelial cells in vitro. Histological analyses demonstrate that the administration of the VEGF-eluting GelMA scaffolds that remain adherent to the wound bed significantly improves the quality of healing in porcine wounds. The introduced in vivo printing strategy for wound healing applications is translational and convenient to use in any place, such as an operating room, and does not require expensive bioprinters or imaging modalities.
format	article
author	Kristo Nuutila Mohamadmahdi Samandari Yori Endo Yuteng Zhang Jacob Quint Tannin A. Schmidt Ali Tamayol Indranil Sinha
author_facet	Kristo Nuutila Mohamadmahdi Samandari Yori Endo Yuteng Zhang Jacob Quint Tannin A. Schmidt Ali Tamayol Indranil Sinha
author_sort	Kristo Nuutila
title	In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing
title_short	In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing
title_full	In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing
title_fullStr	In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing
title_full_unstemmed	In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing
title_sort	in vivo printing of growth factor-eluting adhesive scaffolds improves wound healing
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/32779d4bf58149daa66fc087c6a8e1f1
work_keys_str_mv	AT kristonuutila invivoprintingofgrowthfactorelutingadhesivescaffoldsimproveswoundhealing AT mohamadmahdisamandari invivoprintingofgrowthfactorelutingadhesivescaffoldsimproveswoundhealing AT yoriendo invivoprintingofgrowthfactorelutingadhesivescaffoldsimproveswoundhealing AT yutengzhang invivoprintingofgrowthfactorelutingadhesivescaffoldsimproveswoundhealing AT jacobquint invivoprintingofgrowthfactorelutingadhesivescaffoldsimproveswoundhealing AT tanninaschmidt invivoprintingofgrowthfactorelutingadhesivescaffoldsimproveswoundhealing AT alitamayol invivoprintingofgrowthfactorelutingadhesivescaffoldsimproveswoundhealing AT indranilsinha invivoprintingofgrowthfactorelutingadhesivescaffoldsimproveswoundhealing
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In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing

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