The <named-content content-type="genus-species">Coxiella burnetii</named-content> Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation

ABSTRACT Understanding the molecular pathogenesis of Coxiella burnetii, the causative agent of human Q fever, has historically been hindered by the technical difficulties of genetically manipulating obligate intracellular bacteria. The recent development of culture conditions suitable for axenic pro...

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Autores principales:	Hayley J. Newton, Craig R. Roy
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2011
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/327d4ef623ec45a69360c9bd3f7ac2a6
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spelling	oai:doaj.org-article:327d4ef623ec45a69360c9bd3f7ac2a62021-11-15T15:38:58ZThe <named-content content-type="genus-species">Coxiella burnetii</named-content> Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation10.1128/mBio.00226-112150-7511https://doaj.org/article/327d4ef623ec45a69360c9bd3f7ac2a62011-11-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00226-11https://doaj.org/toc/2150-7511ABSTRACT Understanding the molecular pathogenesis of Coxiella burnetii, the causative agent of human Q fever, has historically been hindered by the technical difficulties of genetically manipulating obligate intracellular bacteria. The recent development of culture conditions suitable for axenic propagation of C. burnetii has paved the way for the application of a range of genetic techniques to address key questions within the field. Recent studies using mutational analysis have revealed that the C. burnetii Dot/Icm type 4 secretion system (T4SS) is an important virulence determinant that is essential for renovation of a lysosome into a mature Coxiella-containing vacuole (CCV) permissive of intracellular replication. Interestingly, a mutant of C. burnetii deficient in Dot/Icm function was found to be capable of replicating within the parasitophorous vacuole created by Leishmania amazonensis, which indicates that C. burnetii replication is not dependent on the cohort of Dot/Icm effector proteins per se but rather that the collective actions of effectors are required to create the specialized niche supportive of replication. Thus, a role for the Dot/Icm T4SS during the intracellular life cycle of C. burnetii has been more clearly defined by these studies, which demonstrate that advances in genetic analysis should allow future studies to focus on the intricacies of Dot/Icm effector functions that facilitate development of the unique CCV.Hayley J. NewtonCraig R. RoyAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 2, Iss 5 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Hayley J. Newton Craig R. Roy The <named-content content-type="genus-species">Coxiella burnetii</named-content> Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation
description	ABSTRACT Understanding the molecular pathogenesis of Coxiella burnetii, the causative agent of human Q fever, has historically been hindered by the technical difficulties of genetically manipulating obligate intracellular bacteria. The recent development of culture conditions suitable for axenic propagation of C. burnetii has paved the way for the application of a range of genetic techniques to address key questions within the field. Recent studies using mutational analysis have revealed that the C. burnetii Dot/Icm type 4 secretion system (T4SS) is an important virulence determinant that is essential for renovation of a lysosome into a mature Coxiella-containing vacuole (CCV) permissive of intracellular replication. Interestingly, a mutant of C. burnetii deficient in Dot/Icm function was found to be capable of replicating within the parasitophorous vacuole created by Leishmania amazonensis, which indicates that C. burnetii replication is not dependent on the cohort of Dot/Icm effector proteins per se but rather that the collective actions of effectors are required to create the specialized niche supportive of replication. Thus, a role for the Dot/Icm T4SS during the intracellular life cycle of C. burnetii has been more clearly defined by these studies, which demonstrate that advances in genetic analysis should allow future studies to focus on the intricacies of Dot/Icm effector functions that facilitate development of the unique CCV.
format	article
author	Hayley J. Newton Craig R. Roy
author_facet	Hayley J. Newton Craig R. Roy
author_sort	Hayley J. Newton
title	The <named-content content-type="genus-species">Coxiella burnetii</named-content> Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation
title_short	The <named-content content-type="genus-species">Coxiella burnetii</named-content> Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation
title_full	The <named-content content-type="genus-species">Coxiella burnetii</named-content> Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation
title_fullStr	The <named-content content-type="genus-species">Coxiella burnetii</named-content> Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation
title_full_unstemmed	The <named-content content-type="genus-species">Coxiella burnetii</named-content> Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation
title_sort	<named-content content-type="genus-species">coxiella burnetii</named-content> dot/icm system creates a comfortable home through lysosomal renovation
publisher	American Society for Microbiology
publishDate	2011
url	https://doaj.org/article/327d4ef623ec45a69360c9bd3f7ac2a6
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The <named-content content-type="genus-species">Coxiella burnetii</named-content> Dot/Icm System Creates a Comfortable Home through Lysosomal Renovation

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