Characterization of the miRNA regulators of the human ovulatory cascade

Abstract Ovarian follicular development and ovulation are complex and tightly regulated processes that involve regulation by microRNAs (miRNAs). We previously identified differentially expressed mRNAs between human cumulus granulosa cells (CGCs) from immature early antral follicles (germinal vesicle...

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Autores principales:	G. M. Yerushalmi, M. Salmon-Divon, L. Ophir, Y. Yung, M. Baum, G. Coticchio, R. Fadini, M. Mignini-Renzini, M. Dal Canto, R. Machtinger, E. Maman, A. Hourvitz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Forkhead Box M1 (FOXM1) Cumulus Granulosa Cells (CGCs) Final Follicular Maturation Ovulatory Process Nuclear Factor Of Activated T-cells (NFAT) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/327d6b3c4340443fb25cb1093138f15f
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spelling	oai:doaj.org-article:327d6b3c4340443fb25cb1093138f15f2021-12-02T15:08:57ZCharacterization of the miRNA regulators of the human ovulatory cascade10.1038/s41598-018-33807-y2045-2322https://doaj.org/article/327d6b3c4340443fb25cb1093138f15f2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33807-yhttps://doaj.org/toc/2045-2322Abstract Ovarian follicular development and ovulation are complex and tightly regulated processes that involve regulation by microRNAs (miRNAs). We previously identified differentially expressed mRNAs between human cumulus granulosa cells (CGCs) from immature early antral follicles (germinal vesicle - GV) and mature preovulatory follicles (metaphase II - M2). In this study, we performed an integrated analysis of the transcriptome and miRNome in CGCs obtained from the GV cumulus-oocyte complex (COC) obtained from IVM and M2 COC obtained from IVF. A total of 43 differentially expressed miRNAs were identified. Using Ingenuity IPA analysis, we identified 7288 potential miRNA-regulated target genes. Two hundred thirty-four of these target genes were also found in our previously generated ovulatory gene library while exhibiting anti-correlated expression to the identified miRNAs. IPA pathway analysis suggested that miR-21 and FOXM1 cooperatively inhibit CDC25A, TOP2A and PRC1. We identified a mechanism for the temporary inhibition of VEGF during ovulation by TGFB1, miR-16-5p and miR-34a-5p. The linkage bioinformatics analysis between the libraries of the coding genes from our preliminary study with the newly generated library of regulatory miRNAs provides us a comprehensive, integrated overview of the miRNA-mRNA co-regulatory networks that may play a key role in controlling post-transcriptomic regulation of the ovulatory process.G. M. YerushalmiM. Salmon-DivonL. OphirY. YungM. BaumG. CoticchioR. FadiniM. Mignini-RenziniM. Dal CantoR. MachtingerE. MamanA. HourvitzNature PortfolioarticleForkhead Box M1 (FOXM1)Cumulus Granulosa Cells (CGCs)Final Follicular MaturationOvulatory ProcessNuclear Factor Of Activated T-cells (NFAT)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Forkhead Box M1 (FOXM1) Cumulus Granulosa Cells (CGCs) Final Follicular Maturation Ovulatory Process Nuclear Factor Of Activated T-cells (NFAT) Medicine R Science Q
spellingShingle	Forkhead Box M1 (FOXM1) Cumulus Granulosa Cells (CGCs) Final Follicular Maturation Ovulatory Process Nuclear Factor Of Activated T-cells (NFAT) Medicine R Science Q G. M. Yerushalmi M. Salmon-Divon L. Ophir Y. Yung M. Baum G. Coticchio R. Fadini M. Mignini-Renzini M. Dal Canto R. Machtinger E. Maman A. Hourvitz Characterization of the miRNA regulators of the human ovulatory cascade
description	Abstract Ovarian follicular development and ovulation are complex and tightly regulated processes that involve regulation by microRNAs (miRNAs). We previously identified differentially expressed mRNAs between human cumulus granulosa cells (CGCs) from immature early antral follicles (germinal vesicle - GV) and mature preovulatory follicles (metaphase II - M2). In this study, we performed an integrated analysis of the transcriptome and miRNome in CGCs obtained from the GV cumulus-oocyte complex (COC) obtained from IVM and M2 COC obtained from IVF. A total of 43 differentially expressed miRNAs were identified. Using Ingenuity IPA analysis, we identified 7288 potential miRNA-regulated target genes. Two hundred thirty-four of these target genes were also found in our previously generated ovulatory gene library while exhibiting anti-correlated expression to the identified miRNAs. IPA pathway analysis suggested that miR-21 and FOXM1 cooperatively inhibit CDC25A, TOP2A and PRC1. We identified a mechanism for the temporary inhibition of VEGF during ovulation by TGFB1, miR-16-5p and miR-34a-5p. The linkage bioinformatics analysis between the libraries of the coding genes from our preliminary study with the newly generated library of regulatory miRNAs provides us a comprehensive, integrated overview of the miRNA-mRNA co-regulatory networks that may play a key role in controlling post-transcriptomic regulation of the ovulatory process.
format	article
author	G. M. Yerushalmi M. Salmon-Divon L. Ophir Y. Yung M. Baum G. Coticchio R. Fadini M. Mignini-Renzini M. Dal Canto R. Machtinger E. Maman A. Hourvitz
author_facet	G. M. Yerushalmi M. Salmon-Divon L. Ophir Y. Yung M. Baum G. Coticchio R. Fadini M. Mignini-Renzini M. Dal Canto R. Machtinger E. Maman A. Hourvitz
author_sort	G. M. Yerushalmi
title	Characterization of the miRNA regulators of the human ovulatory cascade
title_short	Characterization of the miRNA regulators of the human ovulatory cascade
title_full	Characterization of the miRNA regulators of the human ovulatory cascade
title_fullStr	Characterization of the miRNA regulators of the human ovulatory cascade
title_full_unstemmed	Characterization of the miRNA regulators of the human ovulatory cascade
title_sort	characterization of the mirna regulators of the human ovulatory cascade
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/327d6b3c4340443fb25cb1093138f15f
work_keys_str_mv	AT gmyerushalmi characterizationofthemirnaregulatorsofthehumanovulatorycascade AT msalmondivon characterizationofthemirnaregulatorsofthehumanovulatorycascade AT lophir characterizationofthemirnaregulatorsofthehumanovulatorycascade AT yyung characterizationofthemirnaregulatorsofthehumanovulatorycascade AT mbaum characterizationofthemirnaregulatorsofthehumanovulatorycascade AT gcoticchio characterizationofthemirnaregulatorsofthehumanovulatorycascade AT rfadini characterizationofthemirnaregulatorsofthehumanovulatorycascade AT mmigninirenzini characterizationofthemirnaregulatorsofthehumanovulatorycascade AT mdalcanto characterizationofthemirnaregulatorsofthehumanovulatorycascade AT rmachtinger characterizationofthemirnaregulatorsofthehumanovulatorycascade AT emaman characterizationofthemirnaregulatorsofthehumanovulatorycascade AT ahourvitz characterizationofthemirnaregulatorsofthehumanovulatorycascade
_version_	1718387929964871680

Characterization of the miRNA regulators of the human ovulatory cascade

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