Graphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway

Xinxin Yan,1,2 Wen Yang,3 Zengwu Shao,1 Shuhua Yang,1 Xianzhe Liu1 1Department of Orthopaedic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 2Department of Orthopaedic Surgery, Wuhan Third Hospital, 3Department of Anesthesiology, Union Hospital, Tong...

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Autores principales:	Yan XX, Yang W, Shao ZW, Yang SH, Liu XZ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	carbon-based nanomaterials 3D nanostructure stem cell therapy bone formation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/327d9593c61a4997a408b0cab5a43339
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spelling	oai:doaj.org-article:327d9593c61a4997a408b0cab5a433392021-12-02T06:25:50ZGraphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway1178-2013https://doaj.org/article/327d9593c61a4997a408b0cab5a433392016-10-01T00:00:00Zhttps://www.dovepress.com/graphenesingle-walled-carbon-nanotube-hybrids-promoting-osteogenic-dif-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xinxin Yan,1,2 Wen Yang,3 Zengwu Shao,1 Shuhua Yang,1 Xianzhe Liu1 1Department of Orthopaedic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 2Department of Orthopaedic Surgery, Wuhan Third Hospital, 3Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China Abstract: Carbon nanomaterials are becoming increasingly significant in biomedical fields since they exhibit exceptional physicochemical and biocompatible properties. Today, the stem cells offer potentially new therapeutic approaches in tissue engineering and regenerative medicine. However, the induction of differentiation into specific lineages remains challenging, which provoked us to explore the biomedical applications of carbon nanomaterials in stem cells. In this study, we investigated the interactions between graphene/single-walled carbon nanotube (G/SWCNT) hybrids and rat mesenchymal stem cells (rMSCs) and focused on the proliferation and differentiation of rMSCs treated with G/SWCNT hybrids. Cell viability and morphology were evaluated using cell counting kit-8 assay and immunofluorescence staining, respectively. Osteogenic differentiation evaluated by alkaline phosphatase activity of MSCs proved to be higher after treatment with G/SWCNT hybrids, and the mineralized matrix nodule formation was also enhanced. In addition, the expression levels of osteogenic-associated genes were upregulated, while the adipocyte-specific markers were downregulated. Consistent with these results, we illustrated that the effect of G/SWCNT hybrids on the process of osteogenic differentiation of rMSCs can be modulated by activating the p38 signaling pathway and inhibiting the extracellular signal-regulated kinase 1/2 pathway. Nevertheless, our study suggests that carbon nanomaterials offer a promising platform for regenerative medicine in the near future. Keywords: carbon-based nanomaterials, 3D nanostructure, stem cell therapy, bone formationYan XXYang WShao ZWYang SHLiu XZDove Medical Pressarticlecarbon-based nanomaterials3D nanostructurestem cell therapybone formationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5473-5484 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbon-based nanomaterials 3D nanostructure stem cell therapy bone formation Medicine (General) R5-920
spellingShingle	carbon-based nanomaterials 3D nanostructure stem cell therapy bone formation Medicine (General) R5-920 Yan XX Yang W Shao ZW Yang SH Liu XZ Graphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway
description	Xinxin Yan,1,2 Wen Yang,3 Zengwu Shao,1 Shuhua Yang,1 Xianzhe Liu1 1Department of Orthopaedic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 2Department of Orthopaedic Surgery, Wuhan Third Hospital, 3Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China Abstract: Carbon nanomaterials are becoming increasingly significant in biomedical fields since they exhibit exceptional physicochemical and biocompatible properties. Today, the stem cells offer potentially new therapeutic approaches in tissue engineering and regenerative medicine. However, the induction of differentiation into specific lineages remains challenging, which provoked us to explore the biomedical applications of carbon nanomaterials in stem cells. In this study, we investigated the interactions between graphene/single-walled carbon nanotube (G/SWCNT) hybrids and rat mesenchymal stem cells (rMSCs) and focused on the proliferation and differentiation of rMSCs treated with G/SWCNT hybrids. Cell viability and morphology were evaluated using cell counting kit-8 assay and immunofluorescence staining, respectively. Osteogenic differentiation evaluated by alkaline phosphatase activity of MSCs proved to be higher after treatment with G/SWCNT hybrids, and the mineralized matrix nodule formation was also enhanced. In addition, the expression levels of osteogenic-associated genes were upregulated, while the adipocyte-specific markers were downregulated. Consistent with these results, we illustrated that the effect of G/SWCNT hybrids on the process of osteogenic differentiation of rMSCs can be modulated by activating the p38 signaling pathway and inhibiting the extracellular signal-regulated kinase 1/2 pathway. Nevertheless, our study suggests that carbon nanomaterials offer a promising platform for regenerative medicine in the near future. Keywords: carbon-based nanomaterials, 3D nanostructure, stem cell therapy, bone formation
format	article
author	Yan XX Yang W Shao ZW Yang SH Liu XZ
author_facet	Yan XX Yang W Shao ZW Yang SH Liu XZ
author_sort	Yan XX
title	Graphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway
title_short	Graphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway
title_full	Graphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway
title_fullStr	Graphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway
title_full_unstemmed	Graphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway
title_sort	graphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/327d9593c61a4997a408b0cab5a43339
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Graphene/single-walled carbon nanotube hybrids promoting osteogenic differentiation of mesenchymal stem cells by activating p38 signaling pathway

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