A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.

In Saccharomyces cerevisiae, the essential IMP3 gene encodes a component of the SSU processome, a large ribonucleoprotein complex required for processing of small ribosomal subunit RNA precursors. Mutation of the IMP3 termination codon to a sense codon resulted in a viable mutant allele producing a...

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Autores principales:	Bruno Cosnier, Marta Kwapisz, Isabelle Hatin, Olivier Namy, Sylvie Hermann-Le Denmat, Antonin Morillon, Jean-Pierre Rousset, Céline Fabret
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/327deda465694070a02ce828946d1670
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spelling	oai:doaj.org-article:327deda465694070a02ce828946d16702021-11-18T06:54:55ZA viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.1932-620310.1371/journal.pone.0019500https://doaj.org/article/327deda465694070a02ce828946d16702011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21559332/?tool=EBIhttps://doaj.org/toc/1932-6203In Saccharomyces cerevisiae, the essential IMP3 gene encodes a component of the SSU processome, a large ribonucleoprotein complex required for processing of small ribosomal subunit RNA precursors. Mutation of the IMP3 termination codon to a sense codon resulted in a viable mutant allele producing a C-terminal elongated form of the Imp3 protein. A strain expressing the mutant allele displayed ribosome biogenesis defects equivalent to IMP3 depletion. This hypomorphic allele represented a unique opportunity to investigate and better understand the Imp3p functions. We demonstrated that the +1 frameshifting was increased in the mutant strain. Further characterizations revealed involvement of the Imp3 protein in DNA repair and telomere length control, pointing to a functional relationship between both pathways and ribosome biogenesis.Bruno CosnierMarta KwapiszIsabelle HatinOlivier NamySylvie Hermann-Le DenmatAntonin MorillonJean-Pierre RoussetCéline FabretPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e19500 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bruno Cosnier Marta Kwapisz Isabelle Hatin Olivier Namy Sylvie Hermann-Le Denmat Antonin Morillon Jean-Pierre Rousset Céline Fabret A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.
description	In Saccharomyces cerevisiae, the essential IMP3 gene encodes a component of the SSU processome, a large ribonucleoprotein complex required for processing of small ribosomal subunit RNA precursors. Mutation of the IMP3 termination codon to a sense codon resulted in a viable mutant allele producing a C-terminal elongated form of the Imp3 protein. A strain expressing the mutant allele displayed ribosome biogenesis defects equivalent to IMP3 depletion. This hypomorphic allele represented a unique opportunity to investigate and better understand the Imp3p functions. We demonstrated that the +1 frameshifting was increased in the mutant strain. Further characterizations revealed involvement of the Imp3 protein in DNA repair and telomere length control, pointing to a functional relationship between both pathways and ribosome biogenesis.
format	article
author	Bruno Cosnier Marta Kwapisz Isabelle Hatin Olivier Namy Sylvie Hermann-Le Denmat Antonin Morillon Jean-Pierre Rousset Céline Fabret
author_facet	Bruno Cosnier Marta Kwapisz Isabelle Hatin Olivier Namy Sylvie Hermann-Le Denmat Antonin Morillon Jean-Pierre Rousset Céline Fabret
author_sort	Bruno Cosnier
title	A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.
title_short	A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.
title_full	A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.
title_fullStr	A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.
title_full_unstemmed	A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.
title_sort	viable hypomorphic allele of the essential imp3 gene reveals novel protein functions in saccharomyces cerevisiae.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/327deda465694070a02ce828946d1670
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A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.

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