Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system

M Joyce Nirmala,1 Latha Durai,2 Vineet Gopakumar,1 R Nagarajan11Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, India; 2Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600...

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Autores principales:	Nirmala MJ, Durai L, Gopakumar V, Nagarajan R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Clove bud oil Nano-scale emulsion system Thyroid cancer Apoptosis Antibacterial activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/32801de202b542e390e2b12a095ab10e
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spelling	oai:doaj.org-article:32801de202b542e390e2b12a095ab10e2021-12-02T05:03:56ZAnticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system1178-2013https://doaj.org/article/32801de202b542e390e2b12a095ab10e2019-08-01T00:00:00Zhttps://www.dovepress.com/anticancer-and-antibacterial-effects-of-a-clove-bud-essential-oil-base-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013M Joyce Nirmala,1 Latha Durai,2 Vineet Gopakumar,1 R Nagarajan11Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, India; 2Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, IndiaBackground and purpose: The essential oil derived from clove buds (Syzygium aromaticum) has been used as a chemopreventive agent in Ayurvedic medicine. The antiviral, antibacterial, and anticancer properties of its chemo-skeleton have motivated this study to explore its efficacy in pharmaceutics.Methods: Nanoscale-based emulsions were prepared by employing a spontaneous emulsification technique through self-assembly using varying concentrations of Tween 20 and Tween 80 surfactants. Their physicochemical properties and stability were studied in order to choose an optimum formulation which was clear and stable. The cytotoxicity of the stable oil-based emulsion system was evaluated using MTT assay, colony formation assay, and Annexin V-FITC assay against the thyroid cancer cell line (HTh-7).Results: All three methods verified apoptosis and reduction in cancer cell proliferation, making the formulation a promising candidate as an alternative cancer drug. The oil-based emulsion system was also tested for its antibacterial properties against Staphylococcus aureus. Membrane permeability studies proved its efficacy to permeate through cell membrane, thereby increasing the leakage of cytoplasmic contents.Conclusion: Many current treatments for cancers are aggressive yet ineffective. This study positions the clove bud-based nanoscale emulsion as a suitable candidate for further in vivo studies and trials as a cancer drug.Keywords: clove bud oil, nanoscale emulsion system, thyroid cancer, apoptosis, antibacterial activityNirmala MJDurai LGopakumar VNagarajan RDove Medical PressarticleClove bud oilNano-scale emulsion systemThyroid cancerApoptosisAntibacterial activityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 6439-6450 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Clove bud oil Nano-scale emulsion system Thyroid cancer Apoptosis Antibacterial activity Medicine (General) R5-920
spellingShingle	Clove bud oil Nano-scale emulsion system Thyroid cancer Apoptosis Antibacterial activity Medicine (General) R5-920 Nirmala MJ Durai L Gopakumar V Nagarajan R Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system
description	M Joyce Nirmala,1 Latha Durai,2 Vineet Gopakumar,1 R Nagarajan11Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, India; 2Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, IndiaBackground and purpose: The essential oil derived from clove buds (Syzygium aromaticum) has been used as a chemopreventive agent in Ayurvedic medicine. The antiviral, antibacterial, and anticancer properties of its chemo-skeleton have motivated this study to explore its efficacy in pharmaceutics.Methods: Nanoscale-based emulsions were prepared by employing a spontaneous emulsification technique through self-assembly using varying concentrations of Tween 20 and Tween 80 surfactants. Their physicochemical properties and stability were studied in order to choose an optimum formulation which was clear and stable. The cytotoxicity of the stable oil-based emulsion system was evaluated using MTT assay, colony formation assay, and Annexin V-FITC assay against the thyroid cancer cell line (HTh-7).Results: All three methods verified apoptosis and reduction in cancer cell proliferation, making the formulation a promising candidate as an alternative cancer drug. The oil-based emulsion system was also tested for its antibacterial properties against Staphylococcus aureus. Membrane permeability studies proved its efficacy to permeate through cell membrane, thereby increasing the leakage of cytoplasmic contents.Conclusion: Many current treatments for cancers are aggressive yet ineffective. This study positions the clove bud-based nanoscale emulsion as a suitable candidate for further in vivo studies and trials as a cancer drug.Keywords: clove bud oil, nanoscale emulsion system, thyroid cancer, apoptosis, antibacterial activity
format	article
author	Nirmala MJ Durai L Gopakumar V Nagarajan R
author_facet	Nirmala MJ Durai L Gopakumar V Nagarajan R
author_sort	Nirmala MJ
title	Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system
title_short	Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system
title_full	Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system
title_fullStr	Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system
title_full_unstemmed	Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system
title_sort	anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/32801de202b542e390e2b12a095ab10e
work_keys_str_mv	AT nirmalamj anticancerandantibacterialeffectsofaclovebudessentialoilbasednanoscaleemulsionsystem AT durail anticancerandantibacterialeffectsofaclovebudessentialoilbasednanoscaleemulsionsystem AT gopakumarv anticancerandantibacterialeffectsofaclovebudessentialoilbasednanoscaleemulsionsystem AT nagarajanr anticancerandantibacterialeffectsofaclovebudessentialoilbasednanoscaleemulsionsystem
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Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system

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