Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus

Abstract Biofilm formation of Vibrio vulnificus is initiated by adherence of flagellated cells to surfaces, and then flagellum-driven motility is not necessary during biofilm maturation. Once matured biofilms are constructed, cells become flagellated and swim to disperse from biofilms. As a conseque...

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Autores principales:	You-Chul Jung, Mi-Ae Lee, Han-Shin Kim, Kyu-Ho Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/328a6a59f60b4c339e985e22a6baf1d3
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spelling	oai:doaj.org-article:328a6a59f60b4c339e985e22a6baf1d32021-12-02T18:15:35ZRole of DegQ in differential stability of flagellin subunits in Vibrio vulnificus10.1038/s41522-021-00206-72055-5008https://doaj.org/article/328a6a59f60b4c339e985e22a6baf1d32021-04-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00206-7https://doaj.org/toc/2055-5008Abstract Biofilm formation of Vibrio vulnificus is initiated by adherence of flagellated cells to surfaces, and then flagellum-driven motility is not necessary during biofilm maturation. Once matured biofilms are constructed, cells become flagellated and swim to disperse from biofilms. As a consequence, timely regulations of the flagellar components’ expression are crucial to complete a biofilm life-cycle. In this study, we demonstrated that flagellins’ production is regulated in a biofilm stage-specific manner, via activities of a protease DegQ and a chaperone FlaJ. Among four flagellin subunits for V. vulnificus filament, FlaC had the highest affinities to hook-associated proteins, and is critical for maturating flagellum, showed the least susceptibility to DegQ due to the presence of methionine residues in its DegQ-sensitive domains, ND1 and CD0. Therefore, differential regulation by DegQ and FlaJ controls the cytoplasmic stability of flagellins, which further determines the motility-dependent, stage-specific development of biofilms.You-Chul JungMi-Ae LeeHan-Shin KimKyu-Ho LeeNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 You-Chul Jung Mi-Ae Lee Han-Shin Kim Kyu-Ho Lee Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus
description	Abstract Biofilm formation of Vibrio vulnificus is initiated by adherence of flagellated cells to surfaces, and then flagellum-driven motility is not necessary during biofilm maturation. Once matured biofilms are constructed, cells become flagellated and swim to disperse from biofilms. As a consequence, timely regulations of the flagellar components’ expression are crucial to complete a biofilm life-cycle. In this study, we demonstrated that flagellins’ production is regulated in a biofilm stage-specific manner, via activities of a protease DegQ and a chaperone FlaJ. Among four flagellin subunits for V. vulnificus filament, FlaC had the highest affinities to hook-associated proteins, and is critical for maturating flagellum, showed the least susceptibility to DegQ due to the presence of methionine residues in its DegQ-sensitive domains, ND1 and CD0. Therefore, differential regulation by DegQ and FlaJ controls the cytoplasmic stability of flagellins, which further determines the motility-dependent, stage-specific development of biofilms.
format	article
author	You-Chul Jung Mi-Ae Lee Han-Shin Kim Kyu-Ho Lee
author_facet	You-Chul Jung Mi-Ae Lee Han-Shin Kim Kyu-Ho Lee
author_sort	You-Chul Jung
title	Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus
title_short	Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus
title_full	Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus
title_fullStr	Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus
title_full_unstemmed	Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus
title_sort	role of degq in differential stability of flagellin subunits in vibrio vulnificus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/328a6a59f60b4c339e985e22a6baf1d3
work_keys_str_mv	AT youchuljung roleofdegqindifferentialstabilityofflagellinsubunitsinvibriovulnificus AT miaelee roleofdegqindifferentialstabilityofflagellinsubunitsinvibriovulnificus AT hanshinkim roleofdegqindifferentialstabilityofflagellinsubunitsinvibriovulnificus AT kyuholee roleofdegqindifferentialstabilityofflagellinsubunitsinvibriovulnificus
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Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus

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