Is there an inflammatory stimulus to human term labour?

Inflammation is thought to play a pivotal role in the onset of term and some forms of preterm labour. Although, we recently found that myometrial inflammation is a consequence rather than a cause of term labour, there are several other reproductive tissues, including amnion, choriodecidua parietalis...

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Autores principales:	Natasha Singh, Bronwen Herbert, Garvin Sooranna, Nishel M Shah, Ananya Das, Suren R Sooranna, Mark R Johnson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/328d1c012a28468197c7e0f8ee0d0596
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spelling	oai:doaj.org-article:328d1c012a28468197c7e0f8ee0d05962021-12-02T20:14:52ZIs there an inflammatory stimulus to human term labour?1932-620310.1371/journal.pone.0256545https://doaj.org/article/328d1c012a28468197c7e0f8ee0d05962021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256545https://doaj.org/toc/1932-6203Inflammation is thought to play a pivotal role in the onset of term and some forms of preterm labour. Although, we recently found that myometrial inflammation is a consequence rather than a cause of term labour, there are several other reproductive tissues, including amnion, choriodecidua parietalis and decidua basalis, where the inflammatory stimulus to labour may occur. To investigate this, we have obtained amnion, choriodecidual parietalis and decidua basalis samples from women at various stages of pregnancy and spontaneous labour. The inflammatory cytokine profile in each tissue was determine by Bio-Plex Pro® cytokine multiplex assays and quantitative RT-PCR. Active motif assay was used to study transcription activation in the choriodecidua parietalis. Quantitative RT-PCR was use to study the pro-labour genes (PGHS-2, PGDH, OTR and CX43) in all of the tissues at the onset of labour and oxytocin (OT) mRNA expression in the choriodecidual parietalis and decidua basalis. Statistical significance was ascribed to a P value <0.05. In the amnion and choriodecidua parietalis, the mRNA levels of various cytokines decreased from preterm no labour to term no labour samples, but the protein levels were unchanged. The choriodecidua parietalis showed increase in the protein levels of IL-1β and IL-6 in the term early labour samples. In the amnion and decidua basalis, the protein levels of several cytokines rose in term established labour. The multiples of the median derived from the 19-plex cytokine assay were greater in term early labour and term established labour samples from the choriodecidua parietalis, but only in term established labour for myometrium. These data suggest that the inflammatory stimulus to labour may begin in the choriodecidua parietalis, but the absence of any change in prolabour factor mRNA levels suggests that the cytokines may act on the myometrium where we observed changes in transcription factor activation and increases in prolabour gene expression in earlier studies.Natasha SinghBronwen HerbertGarvin SoorannaNishel M ShahAnanya DasSuren R SoorannaMark R JohnsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256545 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natasha Singh Bronwen Herbert Garvin Sooranna Nishel M Shah Ananya Das Suren R Sooranna Mark R Johnson Is there an inflammatory stimulus to human term labour?
description	Inflammation is thought to play a pivotal role in the onset of term and some forms of preterm labour. Although, we recently found that myometrial inflammation is a consequence rather than a cause of term labour, there are several other reproductive tissues, including amnion, choriodecidua parietalis and decidua basalis, where the inflammatory stimulus to labour may occur. To investigate this, we have obtained amnion, choriodecidual parietalis and decidua basalis samples from women at various stages of pregnancy and spontaneous labour. The inflammatory cytokine profile in each tissue was determine by Bio-Plex Pro® cytokine multiplex assays and quantitative RT-PCR. Active motif assay was used to study transcription activation in the choriodecidua parietalis. Quantitative RT-PCR was use to study the pro-labour genes (PGHS-2, PGDH, OTR and CX43) in all of the tissues at the onset of labour and oxytocin (OT) mRNA expression in the choriodecidual parietalis and decidua basalis. Statistical significance was ascribed to a P value <0.05. In the amnion and choriodecidua parietalis, the mRNA levels of various cytokines decreased from preterm no labour to term no labour samples, but the protein levels were unchanged. The choriodecidua parietalis showed increase in the protein levels of IL-1β and IL-6 in the term early labour samples. In the amnion and decidua basalis, the protein levels of several cytokines rose in term established labour. The multiples of the median derived from the 19-plex cytokine assay were greater in term early labour and term established labour samples from the choriodecidua parietalis, but only in term established labour for myometrium. These data suggest that the inflammatory stimulus to labour may begin in the choriodecidua parietalis, but the absence of any change in prolabour factor mRNA levels suggests that the cytokines may act on the myometrium where we observed changes in transcription factor activation and increases in prolabour gene expression in earlier studies.
format	article
author	Natasha Singh Bronwen Herbert Garvin Sooranna Nishel M Shah Ananya Das Suren R Sooranna Mark R Johnson
author_facet	Natasha Singh Bronwen Herbert Garvin Sooranna Nishel M Shah Ananya Das Suren R Sooranna Mark R Johnson
author_sort	Natasha Singh
title	Is there an inflammatory stimulus to human term labour?
title_short	Is there an inflammatory stimulus to human term labour?
title_full	Is there an inflammatory stimulus to human term labour?
title_fullStr	Is there an inflammatory stimulus to human term labour?
title_full_unstemmed	Is there an inflammatory stimulus to human term labour?
title_sort	is there an inflammatory stimulus to human term labour?
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/328d1c012a28468197c7e0f8ee0d0596
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Is there an inflammatory stimulus to human term labour?

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