Identification of a novel nucleocytoplasmic shuttling RNA helicase of trypanosomes.

Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. None...

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Autores principales:	Alexandre Haruo Inoue, Mariana Serpeloni, Priscila Mazzocchi Hiraiwa, Sueli Fumie Yamada-Ogatta, João Renato Carvalho Muniz, Maria Cristina Machado Motta, Newton Medeiros Vidal, Samuel Goldenberg, Andréa Rodrigues Avila
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3290843a5e7d4730a987c7017225391f
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spelling	oai:doaj.org-article:3290843a5e7d4730a987c7017225391f2021-11-25T05:56:46ZIdentification of a novel nucleocytoplasmic shuttling RNA helicase of trypanosomes.1932-620310.1371/journal.pone.0109521https://doaj.org/article/3290843a5e7d4730a987c7017225391f2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109521https://doaj.org/toc/1932-6203Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. Nonetheless, we identified a RNA helicase (Hel45) that is conserved across eukaryotes and similar to shuttling proteins involved in mRNA export. We used in silico analysis to predict the structure of Trypanosoma cruzi Hel45, including the N-terminal domain and the C-terminal domain, and our findings suggest that this RNA helicase can form complexes with mRNA. Hel45 was present in both nucleus and cytoplasm. Electron microscopy showed that Hel45 is clustered close to the cytoplasmic side of nuclear pore complexes, and is also present in the nucleus where it is associated with peripheral compact chromatin. Deletion of a predicted Nuclear Export Signal motif led to the accumulation of Hel45ΔNES in the nucleus, indicating that Hel45 shuttles between the nucleus and the cytoplasm. This transport was dependent on active transcription but did not depend on the exportin Crm1. Knockdown of Mex67 in T. brucei caused the nuclear accumulation of the T. brucei ortholog of Hel45. Indeed, Hel45 is present in mRNA ribonucleoprotein complexes that are not associated with polysomes. It is still necessary to confirm the precise function of Hel45. However, this RNA helicase is associated with mRNA metabolism and its nucleocytoplasmic shuttling is dependent on an mRNA export route involving Mex67 receptor.Alexandre Haruo InoueMariana SerpeloniPriscila Mazzocchi HiraiwaSueli Fumie Yamada-OgattaJoão Renato Carvalho MunizMaria Cristina Machado MottaNewton Medeiros VidalSamuel GoldenbergAndréa Rodrigues AvilaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109521 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandre Haruo Inoue Mariana Serpeloni Priscila Mazzocchi Hiraiwa Sueli Fumie Yamada-Ogatta João Renato Carvalho Muniz Maria Cristina Machado Motta Newton Medeiros Vidal Samuel Goldenberg Andréa Rodrigues Avila Identification of a novel nucleocytoplasmic shuttling RNA helicase of trypanosomes.
description	Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. Nonetheless, we identified a RNA helicase (Hel45) that is conserved across eukaryotes and similar to shuttling proteins involved in mRNA export. We used in silico analysis to predict the structure of Trypanosoma cruzi Hel45, including the N-terminal domain and the C-terminal domain, and our findings suggest that this RNA helicase can form complexes with mRNA. Hel45 was present in both nucleus and cytoplasm. Electron microscopy showed that Hel45 is clustered close to the cytoplasmic side of nuclear pore complexes, and is also present in the nucleus where it is associated with peripheral compact chromatin. Deletion of a predicted Nuclear Export Signal motif led to the accumulation of Hel45ΔNES in the nucleus, indicating that Hel45 shuttles between the nucleus and the cytoplasm. This transport was dependent on active transcription but did not depend on the exportin Crm1. Knockdown of Mex67 in T. brucei caused the nuclear accumulation of the T. brucei ortholog of Hel45. Indeed, Hel45 is present in mRNA ribonucleoprotein complexes that are not associated with polysomes. It is still necessary to confirm the precise function of Hel45. However, this RNA helicase is associated with mRNA metabolism and its nucleocytoplasmic shuttling is dependent on an mRNA export route involving Mex67 receptor.
format	article
author	Alexandre Haruo Inoue Mariana Serpeloni Priscila Mazzocchi Hiraiwa Sueli Fumie Yamada-Ogatta João Renato Carvalho Muniz Maria Cristina Machado Motta Newton Medeiros Vidal Samuel Goldenberg Andréa Rodrigues Avila
author_facet	Alexandre Haruo Inoue Mariana Serpeloni Priscila Mazzocchi Hiraiwa Sueli Fumie Yamada-Ogatta João Renato Carvalho Muniz Maria Cristina Machado Motta Newton Medeiros Vidal Samuel Goldenberg Andréa Rodrigues Avila
author_sort	Alexandre Haruo Inoue
title	Identification of a novel nucleocytoplasmic shuttling RNA helicase of trypanosomes.
title_short	Identification of a novel nucleocytoplasmic shuttling RNA helicase of trypanosomes.
title_full	Identification of a novel nucleocytoplasmic shuttling RNA helicase of trypanosomes.
title_fullStr	Identification of a novel nucleocytoplasmic shuttling RNA helicase of trypanosomes.
title_full_unstemmed	Identification of a novel nucleocytoplasmic shuttling RNA helicase of trypanosomes.
title_sort	identification of a novel nucleocytoplasmic shuttling rna helicase of trypanosomes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3290843a5e7d4730a987c7017225391f
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Identification of a novel nucleocytoplasmic shuttling RNA helicase of trypanosomes.

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