Periodontitis induces endothelial dysfunction in mice

Abstract The treatment of periodontitis has numerous positive effects on established chronic health conditions, including cardiovascular disease and diabetes. However, ethical considerations do limit the establishment of human trials to investigate whether periodontitis promotes the early stages of...

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Autores principales:	Maria Parvaneh, Paul K. Witting, Jaqueline Ku, Tala Moradi, Elif Eroglu, Ben Freedman, Greg T. Sutherland, Andrew McCorkindale, Boris Guennewig, Phannaphat Choowong, Kim Bell-Anderson, Gregory Cooney, Shane R. Thomas, Joerg Eberhard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3290d562eb7d40e5b7d7eca71b22cb2f
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spelling	oai:doaj.org-article:3290d562eb7d40e5b7d7eca71b22cb2f2021-12-02T16:26:38ZPeriodontitis induces endothelial dysfunction in mice10.1038/s41598-021-94418-82045-2322https://doaj.org/article/3290d562eb7d40e5b7d7eca71b22cb2f2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94418-8https://doaj.org/toc/2045-2322Abstract The treatment of periodontitis has numerous positive effects on established chronic health conditions, including cardiovascular disease and diabetes. However, ethical considerations do limit the establishment of human trials to investigate whether periodontitis promotes the early stages of chronic conditions. Therefore, the aim of this study was to investigate whether periodontitis induces endothelial dysfunction in hyperlipidemic apolipoprotein E gene-deficient (ApoE-/-) mice. Forty-five 8-week-old ApoE-/- mice were challenged by oral lavage with Porphyromonas gingivalis and Streptococcus gordonii for 4 weeks. A subgroup of animals (n = 15–17/group) was placed in a metabolic chamber immediately before euthanasia at 4 weeks to measure VO2/CO2 concentrations and voluntary locomotion. In infected and control animals alveolar bone levels were measured by x-ray imaging and endothelial function was determined by measuring endothelial-dependent vasorelaxation of aortic rings. The mRNA expression levels of serum amyloid A and tumor necrosis factor were determined in liver tissues by qRT PCR and protein concentrations in serum by ELISA. Caecal contents were analysed by sequencing to determine changes to the gut microbiota to investigate linkages between microbiome and systemic changes. The results showed that oral lavage of P. gingivalis and S. gordonii for 4 weeks, initiated periodontitis in ApoE-/- mice, similar to the human situation. The oral inflammation was accompanied by a significant increase in mRNA expression of pro-inflammatory mediators serum amyloid A1 and tumor necrosis factor in the liver. Mice with periodontitis also exhibited impaired endothelial-dependent vasorelaxation responses to acetylcholine. This systemic response was connected to increased energy expenditure, locomotion and respiratory quotient. No differences were detected in caecal microbiota between the infected and control animals. Overall, this is the first report that provide evidence that periodontitis induces endothelial dysfunction in mice. Other systemic responses observed in response to the local reaction need further investigation. The study suggests that early prevention of periodontitis may help limit the early stages of endothelial dysfunction that is linked to atherogenesis in humans.Maria ParvanehPaul K. WittingJaqueline KuTala MoradiElif ErogluBen FreedmanGreg T. SutherlandAndrew McCorkindaleBoris GuennewigPhannaphat ChoowongKim Bell-AndersonGregory CooneyShane R. ThomasJoerg EberhardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Parvaneh Paul K. Witting Jaqueline Ku Tala Moradi Elif Eroglu Ben Freedman Greg T. Sutherland Andrew McCorkindale Boris Guennewig Phannaphat Choowong Kim Bell-Anderson Gregory Cooney Shane R. Thomas Joerg Eberhard Periodontitis induces endothelial dysfunction in mice
description	Abstract The treatment of periodontitis has numerous positive effects on established chronic health conditions, including cardiovascular disease and diabetes. However, ethical considerations do limit the establishment of human trials to investigate whether periodontitis promotes the early stages of chronic conditions. Therefore, the aim of this study was to investigate whether periodontitis induces endothelial dysfunction in hyperlipidemic apolipoprotein E gene-deficient (ApoE-/-) mice. Forty-five 8-week-old ApoE-/- mice were challenged by oral lavage with Porphyromonas gingivalis and Streptococcus gordonii for 4 weeks. A subgroup of animals (n = 15–17/group) was placed in a metabolic chamber immediately before euthanasia at 4 weeks to measure VO2/CO2 concentrations and voluntary locomotion. In infected and control animals alveolar bone levels were measured by x-ray imaging and endothelial function was determined by measuring endothelial-dependent vasorelaxation of aortic rings. The mRNA expression levels of serum amyloid A and tumor necrosis factor were determined in liver tissues by qRT PCR and protein concentrations in serum by ELISA. Caecal contents were analysed by sequencing to determine changes to the gut microbiota to investigate linkages between microbiome and systemic changes. The results showed that oral lavage of P. gingivalis and S. gordonii for 4 weeks, initiated periodontitis in ApoE-/- mice, similar to the human situation. The oral inflammation was accompanied by a significant increase in mRNA expression of pro-inflammatory mediators serum amyloid A1 and tumor necrosis factor in the liver. Mice with periodontitis also exhibited impaired endothelial-dependent vasorelaxation responses to acetylcholine. This systemic response was connected to increased energy expenditure, locomotion and respiratory quotient. No differences were detected in caecal microbiota between the infected and control animals. Overall, this is the first report that provide evidence that periodontitis induces endothelial dysfunction in mice. Other systemic responses observed in response to the local reaction need further investigation. The study suggests that early prevention of periodontitis may help limit the early stages of endothelial dysfunction that is linked to atherogenesis in humans.
format	article
author	Maria Parvaneh Paul K. Witting Jaqueline Ku Tala Moradi Elif Eroglu Ben Freedman Greg T. Sutherland Andrew McCorkindale Boris Guennewig Phannaphat Choowong Kim Bell-Anderson Gregory Cooney Shane R. Thomas Joerg Eberhard
author_facet	Maria Parvaneh Paul K. Witting Jaqueline Ku Tala Moradi Elif Eroglu Ben Freedman Greg T. Sutherland Andrew McCorkindale Boris Guennewig Phannaphat Choowong Kim Bell-Anderson Gregory Cooney Shane R. Thomas Joerg Eberhard
author_sort	Maria Parvaneh
title	Periodontitis induces endothelial dysfunction in mice
title_short	Periodontitis induces endothelial dysfunction in mice
title_full	Periodontitis induces endothelial dysfunction in mice
title_fullStr	Periodontitis induces endothelial dysfunction in mice
title_full_unstemmed	Periodontitis induces endothelial dysfunction in mice
title_sort	periodontitis induces endothelial dysfunction in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3290d562eb7d40e5b7d7eca71b22cb2f
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Periodontitis induces endothelial dysfunction in mice

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