IN VITRO ANTI-HIV-1 ACTIVITY OF THE ENZYMATIC EXTRACT ENRICHED WITH LACCASE PRODUCED BY THE FUNGI GANODERMA SP. AND LENTINUS SP.

ABSTRACT Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Laura FLÓREZ-SAMPEDRO, Wildeman ZAPATA, Lina P OROZCO, Amanda I MEJÍA, Carolina ARBOLEDA, María T RUGELES
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia
Materias:	Human immunodeficiency virus type 1 (HIV-1) Ganoderma Lentinus laccase antiviral activity natural products Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/329269ea237d49c2bc8be1805340f120
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:329269ea237d49c2bc8be1805340f120
record_format	dspace
spelling	oai:doaj.org-article:329269ea237d49c2bc8be1805340f1202021-12-02T04:48:06ZIN VITRO ANTI-HIV-1 ACTIVITY OF THE ENZYMATIC EXTRACT ENRICHED WITH LACCASE PRODUCED BY THE FUNGI GANODERMA SP. AND LENTINUS SP.0121-400410.17533/udea.vitae.v23n2a03https://doaj.org/article/329269ea237d49c2bc8be1805340f120http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-40042016000200109&lng=en&tlng=enhttps://doaj.org/toc/0121-4004ABSTRACT Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Objetives: In this study we evaluated the anti-HIV-1 activity of the enzymatic extracts (EE) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EE and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for green fluorescent protein -GFP- and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The EEs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EE from Lentinus sp. had the best selectivity index (SI: 8.3). Conclusions: These results suggest the potential anti-HIV-1 activity of the EE for the exploration of an alternative therapy against HIV-1 infection.Laura FLÓREZ-SAMPEDROWildeman ZAPATALina P OROZCOAmanda I MEJÍACarolina ARBOLEDAMaría T RUGELESUniversidad de AntioquiaarticleHuman immunodeficiency virus type 1 (HIV-1)GanodermaLentinuslaccaseantiviral activitynatural productsFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 23, Iss 2, Pp 109-118
institution	DOAJ
collection	DOAJ
language	EN
topic	Human immunodeficiency virus type 1 (HIV-1) Ganoderma Lentinus laccase antiviral activity natural products Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Human immunodeficiency virus type 1 (HIV-1) Ganoderma Lentinus laccase antiviral activity natural products Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Laura FLÓREZ-SAMPEDRO Wildeman ZAPATA Lina P OROZCO Amanda I MEJÍA Carolina ARBOLEDA María T RUGELES IN VITRO ANTI-HIV-1 ACTIVITY OF THE ENZYMATIC EXTRACT ENRICHED WITH LACCASE PRODUCED BY THE FUNGI GANODERMA SP. AND LENTINUS SP.
description	ABSTRACT Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Objetives: In this study we evaluated the anti-HIV-1 activity of the enzymatic extracts (EE) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EE and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for green fluorescent protein -GFP- and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The EEs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EE from Lentinus sp. had the best selectivity index (SI: 8.3). Conclusions: These results suggest the potential anti-HIV-1 activity of the EE for the exploration of an alternative therapy against HIV-1 infection.
format	article
author	Laura FLÓREZ-SAMPEDRO Wildeman ZAPATA Lina P OROZCO Amanda I MEJÍA Carolina ARBOLEDA María T RUGELES
author_facet	Laura FLÓREZ-SAMPEDRO Wildeman ZAPATA Lina P OROZCO Amanda I MEJÍA Carolina ARBOLEDA María T RUGELES
author_sort	Laura FLÓREZ-SAMPEDRO
title	IN VITRO ANTI-HIV-1 ACTIVITY OF THE ENZYMATIC EXTRACT ENRICHED WITH LACCASE PRODUCED BY THE FUNGI GANODERMA SP. AND LENTINUS SP.
title_short	IN VITRO ANTI-HIV-1 ACTIVITY OF THE ENZYMATIC EXTRACT ENRICHED WITH LACCASE PRODUCED BY THE FUNGI GANODERMA SP. AND LENTINUS SP.
title_full	IN VITRO ANTI-HIV-1 ACTIVITY OF THE ENZYMATIC EXTRACT ENRICHED WITH LACCASE PRODUCED BY THE FUNGI GANODERMA SP. AND LENTINUS SP.
title_fullStr	IN VITRO ANTI-HIV-1 ACTIVITY OF THE ENZYMATIC EXTRACT ENRICHED WITH LACCASE PRODUCED BY THE FUNGI GANODERMA SP. AND LENTINUS SP.
title_full_unstemmed	IN VITRO ANTI-HIV-1 ACTIVITY OF THE ENZYMATIC EXTRACT ENRICHED WITH LACCASE PRODUCED BY THE FUNGI GANODERMA SP. AND LENTINUS SP.
title_sort	in vitro anti-hiv-1 activity of the enzymatic extract enriched with laccase produced by the fungi ganoderma sp. and lentinus sp.
publisher	Universidad de Antioquia
url	https://doaj.org/article/329269ea237d49c2bc8be1805340f120
work_keys_str_mv	AT lauraflorezsampedro invitroantihiv1activityoftheenzymaticextractenrichedwithlaccaseproducedbythefungiganodermaspandlentinussp AT wildemanzapata invitroantihiv1activityoftheenzymaticextractenrichedwithlaccaseproducedbythefungiganodermaspandlentinussp AT linaporozco invitroantihiv1activityoftheenzymaticextractenrichedwithlaccaseproducedbythefungiganodermaspandlentinussp AT amandaimejia invitroantihiv1activityoftheenzymaticextractenrichedwithlaccaseproducedbythefungiganodermaspandlentinussp AT carolinaarboleda invitroantihiv1activityoftheenzymaticextractenrichedwithlaccaseproducedbythefungiganodermaspandlentinussp AT mariatrugeles invitroantihiv1activityoftheenzymaticextractenrichedwithlaccaseproducedbythefungiganodermaspandlentinussp
_version_	1718400998491291648

IN VITRO ANTI-HIV-1 ACTIVITY OF THE ENZYMATIC EXTRACT ENRICHED WITH LACCASE PRODUCED BY THE FUNGI GANODERMA SP. AND LENTINUS SP.

Ejemplares similares