Proteomic analysis reveals rotator cuff injury caused by oxidative stress

Background and aims: Rotator cuff tendinopathy is common and is related to pain and dysfunction. However, the pathological mechanism of rotator cuff injury and shoulder pain is unclear. Objective: to investigate the pathological mechanism of rotator cuff injury and shoulder pain, and screen out the...

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Autores principales:	Tao Yuan, Hong Qian, Xin Yu, Jia Meng, Cheng-Teng Lai, Hui Jiang, Jian-Ning Zhao, Ni-Rong Bao
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/3292a04efc34438386695c9a48edc333
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spelling	oai:doaj.org-article:3292a04efc34438386695c9a48edc3332021-11-12T12:33:23ZProteomic analysis reveals rotator cuff injury caused by oxidative stress2040-623110.1177/2040622320987057https://doaj.org/article/3292a04efc34438386695c9a48edc3332021-03-01T00:00:00Zhttps://doi.org/10.1177/2040622320987057https://doaj.org/toc/2040-6231Background and aims: Rotator cuff tendinopathy is common and is related to pain and dysfunction. However, the pathological mechanism of rotator cuff injury and shoulder pain is unclear. Objective: to investigate the pathological mechanism of rotator cuff injury and shoulder pain, and screen out the marker proteins related to rotator cuff injury by proteomics. Methods: Subacromial synovium specimens were collected from patients undergoing shoulder arthroscopic surgery. The experimental group were patients with rotator cuff repair surgery, and the control group were patients with habitual dislocation of the shoulder joint. Pathological examination was performed, and then followed by non-labeled quantitative proteomic detection. Finally, from analysis of the biological information of the samples, specific proteins related to rotator cuff injury and shoulder pain were deduced by functional analysis of differential proteins. Results: All the patients in experimental groups were representative. A large number of adipocytes and inflammatory cells were found in the pathological sections of the experimental group; the proteomics analysis screen identified 80 proteins with significant differences, and the analysis of protein function revealed that S100A11 ( p = 0.011), PLIN4 ( p = 0.017), HYOU1 ( p = 0.002) and CLIC1 ( p = 0.007) were closely related to oxidative stress and chronic inflammation. Conclusion: Rotator cuff injury is closely related to oxidative stress and chronic inflammatory response, and the results suggest that the expression of S100A11, PLIN4, HYOU1 and CLIC1 in the synovium of rotator cuff injury provides a new marker for the study of its pathological mechanism.Tao YuanHong QianXin YuJia MengCheng-Teng LaiHui JiangJian-Ning ZhaoNi-Rong BaoSAGE PublishingarticleTherapeutics. PharmacologyRM1-950ENTherapeutic Advances in Chronic Disease, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Therapeutics. Pharmacology RM1-950
spellingShingle	Therapeutics. Pharmacology RM1-950 Tao Yuan Hong Qian Xin Yu Jia Meng Cheng-Teng Lai Hui Jiang Jian-Ning Zhao Ni-Rong Bao Proteomic analysis reveals rotator cuff injury caused by oxidative stress
description	Background and aims: Rotator cuff tendinopathy is common and is related to pain and dysfunction. However, the pathological mechanism of rotator cuff injury and shoulder pain is unclear. Objective: to investigate the pathological mechanism of rotator cuff injury and shoulder pain, and screen out the marker proteins related to rotator cuff injury by proteomics. Methods: Subacromial synovium specimens were collected from patients undergoing shoulder arthroscopic surgery. The experimental group were patients with rotator cuff repair surgery, and the control group were patients with habitual dislocation of the shoulder joint. Pathological examination was performed, and then followed by non-labeled quantitative proteomic detection. Finally, from analysis of the biological information of the samples, specific proteins related to rotator cuff injury and shoulder pain were deduced by functional analysis of differential proteins. Results: All the patients in experimental groups were representative. A large number of adipocytes and inflammatory cells were found in the pathological sections of the experimental group; the proteomics analysis screen identified 80 proteins with significant differences, and the analysis of protein function revealed that S100A11 ( p = 0.011), PLIN4 ( p = 0.017), HYOU1 ( p = 0.002) and CLIC1 ( p = 0.007) were closely related to oxidative stress and chronic inflammation. Conclusion: Rotator cuff injury is closely related to oxidative stress and chronic inflammatory response, and the results suggest that the expression of S100A11, PLIN4, HYOU1 and CLIC1 in the synovium of rotator cuff injury provides a new marker for the study of its pathological mechanism.
format	article
author	Tao Yuan Hong Qian Xin Yu Jia Meng Cheng-Teng Lai Hui Jiang Jian-Ning Zhao Ni-Rong Bao
author_facet	Tao Yuan Hong Qian Xin Yu Jia Meng Cheng-Teng Lai Hui Jiang Jian-Ning Zhao Ni-Rong Bao
author_sort	Tao Yuan
title	Proteomic analysis reveals rotator cuff injury caused by oxidative stress
title_short	Proteomic analysis reveals rotator cuff injury caused by oxidative stress
title_full	Proteomic analysis reveals rotator cuff injury caused by oxidative stress
title_fullStr	Proteomic analysis reveals rotator cuff injury caused by oxidative stress
title_full_unstemmed	Proteomic analysis reveals rotator cuff injury caused by oxidative stress
title_sort	proteomic analysis reveals rotator cuff injury caused by oxidative stress
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/3292a04efc34438386695c9a48edc333
work_keys_str_mv	AT taoyuan proteomicanalysisrevealsrotatorcuffinjurycausedbyoxidativestress AT hongqian proteomicanalysisrevealsrotatorcuffinjurycausedbyoxidativestress AT xinyu proteomicanalysisrevealsrotatorcuffinjurycausedbyoxidativestress AT jiameng proteomicanalysisrevealsrotatorcuffinjurycausedbyoxidativestress AT chengtenglai proteomicanalysisrevealsrotatorcuffinjurycausedbyoxidativestress AT huijiang proteomicanalysisrevealsrotatorcuffinjurycausedbyoxidativestress AT jianningzhao proteomicanalysisrevealsrotatorcuffinjurycausedbyoxidativestress AT nirongbao proteomicanalysisrevealsrotatorcuffinjurycausedbyoxidativestress
_version_	1718430487676977152

Proteomic analysis reveals rotator cuff injury caused by oxidative stress

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