Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

Numerous strategies exist to limit the off-target activity of CRISPR-Cas9 nucleases. Here the authors co-administer truncated gRNAs that block both Cas9 and high-fidelity Cas9 variants from cleaving at off-target sites.

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Detalles Bibliográficos
Autores principales:	John C. Rose, Nicholas A. Popp, Christopher D. Richardson, Jason J. Stephany, Julie Mathieu, Cindy T. Wei, Jacob E. Corn, Dustin J. Maly, Douglas M. Fowler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/32953ad84ae148f1a96180fcc11e3524
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spelling	oai:doaj.org-article:32953ad84ae148f1a96180fcc11e35242021-12-02T15:02:47ZSuppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs10.1038/s41467-020-16542-92041-1723https://doaj.org/article/32953ad84ae148f1a96180fcc11e35242020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16542-9https://doaj.org/toc/2041-1723Numerous strategies exist to limit the off-target activity of CRISPR-Cas9 nucleases. Here the authors co-administer truncated gRNAs that block both Cas9 and high-fidelity Cas9 variants from cleaving at off-target sites.John C. RoseNicholas A. PoppChristopher D. RichardsonJason J. StephanyJulie MathieuCindy T. WeiJacob E. CornDustin J. MalyDouglas M. FowlerNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q John C. Rose Nicholas A. Popp Christopher D. Richardson Jason J. Stephany Julie Mathieu Cindy T. Wei Jacob E. Corn Dustin J. Maly Douglas M. Fowler Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs
description	Numerous strategies exist to limit the off-target activity of CRISPR-Cas9 nucleases. Here the authors co-administer truncated gRNAs that block both Cas9 and high-fidelity Cas9 variants from cleaving at off-target sites.
format	article
author	John C. Rose Nicholas A. Popp Christopher D. Richardson Jason J. Stephany Julie Mathieu Cindy T. Wei Jacob E. Corn Dustin J. Maly Douglas M. Fowler
author_facet	John C. Rose Nicholas A. Popp Christopher D. Richardson Jason J. Stephany Julie Mathieu Cindy T. Wei Jacob E. Corn Dustin J. Maly Douglas M. Fowler
author_sort	John C. Rose
title	Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs
title_short	Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs
title_full	Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs
title_fullStr	Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs
title_full_unstemmed	Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs
title_sort	suppression of unwanted crispr-cas9 editing by co-administration of catalytically inactivating truncated guide rnas
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/32953ad84ae148f1a96180fcc11e3524
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Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

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