Tim-3 expression in cervical cancer promotes tumor metastasis.

<h4>Background</h4>T cell immunoglobulin mucin-3 (Tim-3) has been identified as a negative regulator of anti-tumor immunity. Recent studies highlight the important role of Tim-3 in the CD8(+) T cell exhaustion that takes place in both human and animal cancer models. However, the nature o...

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Autores principales:	Yang Cao, Xiaoxi Zhou, Xiaoyuan Huang, Qinlu Li, Lili Gao, Lijun Jiang, Mei Huang, Jianfeng Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/32955eba1a0f4020a8555c010cd72313
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spelling	oai:doaj.org-article:32955eba1a0f4020a8555c010cd723132021-11-18T08:01:24ZTim-3 expression in cervical cancer promotes tumor metastasis.1932-620310.1371/journal.pone.0053834https://doaj.org/article/32955eba1a0f4020a8555c010cd723132013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23335978/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>T cell immunoglobulin mucin-3 (Tim-3) has been identified as a negative regulator of anti-tumor immunity. Recent studies highlight the important role of Tim-3 in the CD8(+) T cell exhaustion that takes place in both human and animal cancer models. However, the nature of Tim-3 expression in the tumor cell and the mechanism by which it inhibits anti-tumor immunity are unclear. This present study aims to determine Tim-3 is expressed in cervical cancer cells and to evaluate the role of Tim-3 in cervical cancer progression.<h4>Methodology</h4>A total of 85 cervical tissue specimens including 43 human cervical cancer, 22 cervical intraepithelial neoplasia (CIN) and 20 chronic cervicitis were involved. Tim-3 expression in tumor cells was detected and was found to correlate with clinicopathological parameters. Meanwhile, expression of Tim-3 was assessed by RT-PCR, Western Blot and confocal microscopy in cervical cancer cell lines, HeLa and SiHa. The migration and invasion potential of Hela cells was evaluated after inhibiting Tim-3 expression by ADV-antisense Tim-3.<h4>Conclusions</h4>We found that Tim-3 was expressed at a higher level in the clinical cervical cancer cells compared to the CIN and chronic cervicitis controls. We supported this finding by confirming the presence of Tim-3 mRNA and protein in the cervical cell lines. Tim-3 expression in tumor cells correlated with clinicopathological parameters. Patients with high expression of Tim-3 had a significant metastatic potential, advanced cancer grades and shorter overall survival than those with lower expression. Multivariate analysis showed that Tim-3 expression was an independent factor for predicting the prognosis of cervical cancer. Significantly, down-regulating the expression of Tim-3 protein inhibited migration and invasion of Hela cells. Our study suggests that the expression of Tim-3 in tumor cells may be an independent prognostic factor for patients with cervical cancer. Moreover, Tim-3 expression may promote metastatic potential in cervical cancers.Yang CaoXiaoxi ZhouXiaoyuan HuangQinlu LiLili GaoLijun JiangMei HuangJianfeng ZhouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53834 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Cao Xiaoxi Zhou Xiaoyuan Huang Qinlu Li Lili Gao Lijun Jiang Mei Huang Jianfeng Zhou Tim-3 expression in cervical cancer promotes tumor metastasis.
description	<h4>Background</h4>T cell immunoglobulin mucin-3 (Tim-3) has been identified as a negative regulator of anti-tumor immunity. Recent studies highlight the important role of Tim-3 in the CD8(+) T cell exhaustion that takes place in both human and animal cancer models. However, the nature of Tim-3 expression in the tumor cell and the mechanism by which it inhibits anti-tumor immunity are unclear. This present study aims to determine Tim-3 is expressed in cervical cancer cells and to evaluate the role of Tim-3 in cervical cancer progression.<h4>Methodology</h4>A total of 85 cervical tissue specimens including 43 human cervical cancer, 22 cervical intraepithelial neoplasia (CIN) and 20 chronic cervicitis were involved. Tim-3 expression in tumor cells was detected and was found to correlate with clinicopathological parameters. Meanwhile, expression of Tim-3 was assessed by RT-PCR, Western Blot and confocal microscopy in cervical cancer cell lines, HeLa and SiHa. The migration and invasion potential of Hela cells was evaluated after inhibiting Tim-3 expression by ADV-antisense Tim-3.<h4>Conclusions</h4>We found that Tim-3 was expressed at a higher level in the clinical cervical cancer cells compared to the CIN and chronic cervicitis controls. We supported this finding by confirming the presence of Tim-3 mRNA and protein in the cervical cell lines. Tim-3 expression in tumor cells correlated with clinicopathological parameters. Patients with high expression of Tim-3 had a significant metastatic potential, advanced cancer grades and shorter overall survival than those with lower expression. Multivariate analysis showed that Tim-3 expression was an independent factor for predicting the prognosis of cervical cancer. Significantly, down-regulating the expression of Tim-3 protein inhibited migration and invasion of Hela cells. Our study suggests that the expression of Tim-3 in tumor cells may be an independent prognostic factor for patients with cervical cancer. Moreover, Tim-3 expression may promote metastatic potential in cervical cancers.
format	article
author	Yang Cao Xiaoxi Zhou Xiaoyuan Huang Qinlu Li Lili Gao Lijun Jiang Mei Huang Jianfeng Zhou
author_facet	Yang Cao Xiaoxi Zhou Xiaoyuan Huang Qinlu Li Lili Gao Lijun Jiang Mei Huang Jianfeng Zhou
author_sort	Yang Cao
title	Tim-3 expression in cervical cancer promotes tumor metastasis.
title_short	Tim-3 expression in cervical cancer promotes tumor metastasis.
title_full	Tim-3 expression in cervical cancer promotes tumor metastasis.
title_fullStr	Tim-3 expression in cervical cancer promotes tumor metastasis.
title_full_unstemmed	Tim-3 expression in cervical cancer promotes tumor metastasis.
title_sort	tim-3 expression in cervical cancer promotes tumor metastasis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/32955eba1a0f4020a8555c010cd72313
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_version_	1718422595882188800

Tim-3 expression in cervical cancer promotes tumor metastasis.

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