The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice

Soyoung Lee,1,* Mi-Sun Kim,1,* Dakeun Lee,2 Taeg Kyu Kwon,3 Dongwoo Khang,4 Hui-Suk Yun,5 Sang-Hyun Kim11CMRI, Laboratory of Immunotoxicology, Department of Pharmacology,School of Medicine, Kyungpook National University, Daegu, Republic of Korea; 2Department of Pathology, School of Medicine, Kyungpo...

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Autores principales:	Lee S, Kim MS, Lee D, Kwon TK, Khang D, Yun HS, Kim SH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/329a8e6e9b1548f1aabfe6d8d5e90576
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spelling	oai:doaj.org-article:329a8e6e9b1548f1aabfe6d8d5e905762021-12-02T02:31:37ZThe comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice1176-91141178-2013https://doaj.org/article/329a8e6e9b1548f1aabfe6d8d5e905762013-01-01T00:00:00Zhttp://www.dovepress.com/the-comparative-immunotoxicity-of-mesoporous-silica-nanoparticles-and--a11878https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Soyoung Lee,1,* Mi-Sun Kim,1,* Dakeun Lee,2 Taeg Kyu Kwon,3 Dongwoo Khang,4 Hui-Suk Yun,5 Sang-Hyun Kim11CMRI, Laboratory of Immunotoxicology, Department of Pharmacology,School of Medicine, Kyungpook National University, Daegu, Republic of Korea; 2Department of Pathology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; 3Department of Immunology, School of Medicine, Keimyung University, Daegu, Republic of Korea; 4School of Nano and Advanced Materials Science and Engineering, Gyeongsang National University, Jinju, Republic of Korea; 5Engineering Ceramics Department, Powder and Ceramics Division, Korea Institute of Materials Science, Changwon, Republic of Korea*These authors contributed equally to this workBackground: Mesoporous silica (MPS) nanoparticles (NPs), which have a unique pore structure and extremely large surface area and pore volume, have received much attention because of their biomedical application potential. Using MPS NPs for biomedical devices requires the verification of their biocompatibility because the surface area of NPs is one of the most important determinants of toxicity, including the cellular uptake and immune response. We have previously reported that the cytotoxicity and inflammation potential of MPS NPs have been shown to be lower than those of general amorphous colloidal silica (Col) NPs in macrophages, but the low cytotoxicity does not guarantee high biocompatibility in vivo. In this study, we compared the in vivo immunotoxicity of MPS and Col NPs in the mouse model to define the effects of pore structural conditions of silica NPs.Materials and methods: Both MPS and Col NPs (2, 20, and 50 mg/kg/day) were intraperitoneally administered in female BALB/c mice for 4 weeks, and clinical toxicity, lymphocyte population, serum IgG/IgM levels, and histological changes were examined.Results: There was no overt sign of clinical toxicity in either MPS- or Col-treated mice. However, MPS NPs led to significant increases in liver and spleen weight and splenocyte proliferation. Mice treated with MPS NPs showed altered lymphocyte populations (CD3+, CD45+, CD4+, and CD8+) in the spleen, increased serum IgG and IgM levels, and histological changes. Despite slight changes in lymphocyte populations in the spleen, Col NPs did not alter other immunological factors.Conclusion: The results indicate that in vivo exposure to MPS NPs caused more damage to systemic immunity than that of Col NPs through the dysregulation of the spleen. The results for in vivo data are inconsistent with those for in vitro data, which show lower cytotoxicity for MPS NPs. These results suggest the importance of verifying biocompatibility both in vitro and in vivo during the design of new nanomaterials.Keywords: immunotoxicity, mesoporous silica nanoparticle, colloidal silica nanoparticle, spleenLee SKim MSLee DKwon TKKhang DYun HSKim SHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 147-158 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lee S Kim MS Lee D Kwon TK Khang D Yun HS Kim SH The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice
description	Soyoung Lee,1,* Mi-Sun Kim,1,* Dakeun Lee,2 Taeg Kyu Kwon,3 Dongwoo Khang,4 Hui-Suk Yun,5 Sang-Hyun Kim11CMRI, Laboratory of Immunotoxicology, Department of Pharmacology,School of Medicine, Kyungpook National University, Daegu, Republic of Korea; 2Department of Pathology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; 3Department of Immunology, School of Medicine, Keimyung University, Daegu, Republic of Korea; 4School of Nano and Advanced Materials Science and Engineering, Gyeongsang National University, Jinju, Republic of Korea; 5Engineering Ceramics Department, Powder and Ceramics Division, Korea Institute of Materials Science, Changwon, Republic of Korea*These authors contributed equally to this workBackground: Mesoporous silica (MPS) nanoparticles (NPs), which have a unique pore structure and extremely large surface area and pore volume, have received much attention because of their biomedical application potential. Using MPS NPs for biomedical devices requires the verification of their biocompatibility because the surface area of NPs is one of the most important determinants of toxicity, including the cellular uptake and immune response. We have previously reported that the cytotoxicity and inflammation potential of MPS NPs have been shown to be lower than those of general amorphous colloidal silica (Col) NPs in macrophages, but the low cytotoxicity does not guarantee high biocompatibility in vivo. In this study, we compared the in vivo immunotoxicity of MPS and Col NPs in the mouse model to define the effects of pore structural conditions of silica NPs.Materials and methods: Both MPS and Col NPs (2, 20, and 50 mg/kg/day) were intraperitoneally administered in female BALB/c mice for 4 weeks, and clinical toxicity, lymphocyte population, serum IgG/IgM levels, and histological changes were examined.Results: There was no overt sign of clinical toxicity in either MPS- or Col-treated mice. However, MPS NPs led to significant increases in liver and spleen weight and splenocyte proliferation. Mice treated with MPS NPs showed altered lymphocyte populations (CD3+, CD45+, CD4+, and CD8+) in the spleen, increased serum IgG and IgM levels, and histological changes. Despite slight changes in lymphocyte populations in the spleen, Col NPs did not alter other immunological factors.Conclusion: The results indicate that in vivo exposure to MPS NPs caused more damage to systemic immunity than that of Col NPs through the dysregulation of the spleen. The results for in vivo data are inconsistent with those for in vitro data, which show lower cytotoxicity for MPS NPs. These results suggest the importance of verifying biocompatibility both in vitro and in vivo during the design of new nanomaterials.Keywords: immunotoxicity, mesoporous silica nanoparticle, colloidal silica nanoparticle, spleen
format	article
author	Lee S Kim MS Lee D Kwon TK Khang D Yun HS Kim SH
author_facet	Lee S Kim MS Lee D Kwon TK Khang D Yun HS Kim SH
author_sort	Lee S
title	The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice
title_short	The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice
title_full	The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice
title_fullStr	The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice
title_full_unstemmed	The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice
title_sort	comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/329a8e6e9b1548f1aabfe6d8d5e90576
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The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice

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