Image quality guided smart rotation improves coverage in microscopy

Multi-view SPIM imaging can improve coverage of large samples such as whole embryos, but the procedure increases phototoxicity and involves manual steps that can introduce inconsistencies. Here the authors develop a smart rotation workflow that performs on-the-fly image analysis and identifies optim...

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Autores principales:	Jiaye He, Jan Huisken
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/329beafeb1c142f4b47bb3a22494fd16
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spelling	oai:doaj.org-article:329beafeb1c142f4b47bb3a22494fd162021-12-02T16:50:01ZImage quality guided smart rotation improves coverage in microscopy10.1038/s41467-019-13821-y2041-1723https://doaj.org/article/329beafeb1c142f4b47bb3a22494fd162020-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13821-yhttps://doaj.org/toc/2041-1723Multi-view SPIM imaging can improve coverage of large samples such as whole embryos, but the procedure increases phototoxicity and involves manual steps that can introduce inconsistencies. Here the authors develop a smart rotation workflow that performs on-the-fly image analysis and identifies optimal set of views to maximize sample coverage.Jiaye HeJan HuiskenNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jiaye He Jan Huisken Image quality guided smart rotation improves coverage in microscopy
description	Multi-view SPIM imaging can improve coverage of large samples such as whole embryos, but the procedure increases phototoxicity and involves manual steps that can introduce inconsistencies. Here the authors develop a smart rotation workflow that performs on-the-fly image analysis and identifies optimal set of views to maximize sample coverage.
format	article
author	Jiaye He Jan Huisken
author_facet	Jiaye He Jan Huisken
author_sort	Jiaye He
title	Image quality guided smart rotation improves coverage in microscopy
title_short	Image quality guided smart rotation improves coverage in microscopy
title_full	Image quality guided smart rotation improves coverage in microscopy
title_fullStr	Image quality guided smart rotation improves coverage in microscopy
title_full_unstemmed	Image quality guided smart rotation improves coverage in microscopy
title_sort	image quality guided smart rotation improves coverage in microscopy
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/329beafeb1c142f4b47bb3a22494fd16
work_keys_str_mv	AT jiayehe imagequalityguidedsmartrotationimprovescoverageinmicroscopy AT janhuisken imagequalityguidedsmartrotationimprovescoverageinmicroscopy
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Image quality guided smart rotation improves coverage in microscopy

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