Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study

Zohre Rezaee,1,2 Ali Yadollahpour,1,2 Vahid Bayati,1 Fereshteh Negad Dehbashi1 1Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Avhaz, Iran; 2Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz...

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Autores principales:	Rezaee Z, Yadollahpour A, Bayati V, Negad Dehbashi F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Gold Nanoparticles Radiosensitizer Electroporation Dose Enhancement Factor Synergistic Effect Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/329c25ed7de74a21b063202dac8075e0
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spelling	oai:doaj.org-article:329c25ed7de74a21b063202dac8075e02021-12-02T03:55:35ZGold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study1178-2013https://doaj.org/article/329c25ed7de74a21b063202dac8075e02017-02-01T00:00:00Zhttps://www.dovepress.com/gold-nanoparticles-and-electroporation-impose-both-separate-and-synerg-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zohre Rezaee,1,2 Ali Yadollahpour,1,2 Vahid Bayati,1 Fereshteh Negad Dehbashi1 1Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Avhaz, Iran; 2Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Background and objective: Radiation therapy (RT) is the gold standard treatment for more than half of known tumors. Despite recent improvements in RT efficiency, the side effects of ionizing radiation (IR) in normal tissues are a dose-limiting factor that restricts higher doses in tumor treatment. One approach to enhance the efficiency of RT is the application of radiosensitizers to selectively increase the dose at the tumor site. Gold nanoparticles (GNPs) and electroporation (EP) have shown good potential as radiosensitizers for RT. This study aims to investigate the sensitizing effects of EP, GNPs, and combined GNPs-EP on the dose enhancement factor (DEF) for 6 MV photon energy. Methods: Radiosensitizing effects of EP, GNPs, and combinations of GNPs-EP were comparatively investigated in vitro for intestinal colon cancer (HT-29) and Chinese hamster ovary (CHO) cell lines by MTT assay and colony formation assay at 6 MV photon energy in six groups: IR (control group), GNPs+IR, GNPs (24 h)+IR, EP+IR, GNPs+EP+IR, and GNPs (24 h)+EP+IR. Results: Treatment of both cell lines with EP, GNPs, and combined GNPs-EP significantly enhanced the response of cells to irradiation. However, the HT-29 showed higher DEF values for all groups. In addition, the DEF value for HT-29 cells for GNPs+IR, GNPs (24 h)+IR, EP+IR, GNPs+EP+IR, and GNPs (24 h)+EP+IR was, respectively, 1.17, 1.47, 1.36, 2.61, and 2.89, indicating synergistic radiosensitizing effect for the GNPs (24 h)+EP+IR group. Furthermore, the synergistic effect was observed just for HT-29 tumor cell lines. Conclusion: Combined GNPs-EP protocols induced synergistic radiosensitizing effect in HT-29 cells, and the effect is also tumor specific. This combined therapy can be beneficially used for the treatment of intrinsically less radiosensitive tumors. Keywords: gold nanoparticles, radiosensitizer, electroporation, dose enhancement factor, synergistic effectRezaee ZYadollahpour ABayati VNegad Dehbashi FDove Medical PressarticleGold NanoparticlesRadiosensitizerElectroporationDose Enhancement FactorSynergistic EffectMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1431-1439 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gold Nanoparticles Radiosensitizer Electroporation Dose Enhancement Factor Synergistic Effect Medicine (General) R5-920
spellingShingle	Gold Nanoparticles Radiosensitizer Electroporation Dose Enhancement Factor Synergistic Effect Medicine (General) R5-920 Rezaee Z Yadollahpour A Bayati V Negad Dehbashi F Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study
description	Zohre Rezaee,1,2 Ali Yadollahpour,1,2 Vahid Bayati,1 Fereshteh Negad Dehbashi1 1Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Avhaz, Iran; 2Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Background and objective: Radiation therapy (RT) is the gold standard treatment for more than half of known tumors. Despite recent improvements in RT efficiency, the side effects of ionizing radiation (IR) in normal tissues are a dose-limiting factor that restricts higher doses in tumor treatment. One approach to enhance the efficiency of RT is the application of radiosensitizers to selectively increase the dose at the tumor site. Gold nanoparticles (GNPs) and electroporation (EP) have shown good potential as radiosensitizers for RT. This study aims to investigate the sensitizing effects of EP, GNPs, and combined GNPs-EP on the dose enhancement factor (DEF) for 6 MV photon energy. Methods: Radiosensitizing effects of EP, GNPs, and combinations of GNPs-EP were comparatively investigated in vitro for intestinal colon cancer (HT-29) and Chinese hamster ovary (CHO) cell lines by MTT assay and colony formation assay at 6 MV photon energy in six groups: IR (control group), GNPs+IR, GNPs (24 h)+IR, EP+IR, GNPs+EP+IR, and GNPs (24 h)+EP+IR. Results: Treatment of both cell lines with EP, GNPs, and combined GNPs-EP significantly enhanced the response of cells to irradiation. However, the HT-29 showed higher DEF values for all groups. In addition, the DEF value for HT-29 cells for GNPs+IR, GNPs (24 h)+IR, EP+IR, GNPs+EP+IR, and GNPs (24 h)+EP+IR was, respectively, 1.17, 1.47, 1.36, 2.61, and 2.89, indicating synergistic radiosensitizing effect for the GNPs (24 h)+EP+IR group. Furthermore, the synergistic effect was observed just for HT-29 tumor cell lines. Conclusion: Combined GNPs-EP protocols induced synergistic radiosensitizing effect in HT-29 cells, and the effect is also tumor specific. This combined therapy can be beneficially used for the treatment of intrinsically less radiosensitive tumors. Keywords: gold nanoparticles, radiosensitizer, electroporation, dose enhancement factor, synergistic effect
format	article
author	Rezaee Z Yadollahpour A Bayati V Negad Dehbashi F
author_facet	Rezaee Z Yadollahpour A Bayati V Negad Dehbashi F
author_sort	Rezaee Z
title	Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study
title_short	Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study
title_full	Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study
title_fullStr	Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study
title_full_unstemmed	Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study
title_sort	gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in ht-29 tumor cells: an in vitro study
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/329c25ed7de74a21b063202dac8075e0
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Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study

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