White blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.

Prion transmission can occur by blood transfusion in human variant Creutzfeldt-Jakob disease and in experimental animal models, including sheep. Screening of blood and its derivatives for the presence of prions became therefore a major public health issue. As infectious titer in blood is reportedly...

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Autores principales:	Sophie Halliez, Emilie Jaumain, Alvina Huor, Jean-Yves Douet, Séverine Lugan, Hervé Cassard, Caroline Lacroux, Vincent Béringue, Olivier Andréoletti, Didier Vilette
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/329de7c6b9af4ceaa86eb3c5f1445ec3
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spelling	oai:doaj.org-article:329de7c6b9af4ceaa86eb3c5f1445ec32021-11-25T06:04:39ZWhite blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.1932-620310.1371/journal.pone.0104287https://doaj.org/article/329de7c6b9af4ceaa86eb3c5f1445ec32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25122456/?tool=EBIhttps://doaj.org/toc/1932-6203Prion transmission can occur by blood transfusion in human variant Creutzfeldt-Jakob disease and in experimental animal models, including sheep. Screening of blood and its derivatives for the presence of prions became therefore a major public health issue. As infectious titer in blood is reportedly low, highly sensitive and robust methods are required to detect prions in blood and blood derived products. The objectives of this study were to compare different methods--in vitro, ex vivo and in vivo assays--to detect prion infectivity in cells prepared from blood samples obtained from scrapie infected sheep at different time points of the disease. Protein misfolding cyclic amplification (PMCA) and bioassays in transgenic mice expressing the ovine prion protein were the most efficient methods to identify infected animals at any time of the disease (asymptomatic to terminally-ill stages). However scrapie cell and cerebellar organotypic slice culture assays designed to replicate ovine prions in culture also allowed detection of prion infectivity in blood cells from asymptomatic sheep. These findings confirm that white blood cells are appropriate targets for preclinical detection and introduce ex vivo tools to detect blood infectivity during the asymptomatic stage of the disease.Sophie HalliezEmilie JaumainAlvina HuorJean-Yves DouetSéverine LuganHervé CassardCaroline LacrouxVincent BéringueOlivier AndréolettiDidier VilettePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104287 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sophie Halliez Emilie Jaumain Alvina Huor Jean-Yves Douet Séverine Lugan Hervé Cassard Caroline Lacroux Vincent Béringue Olivier Andréoletti Didier Vilette White blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.
description	Prion transmission can occur by blood transfusion in human variant Creutzfeldt-Jakob disease and in experimental animal models, including sheep. Screening of blood and its derivatives for the presence of prions became therefore a major public health issue. As infectious titer in blood is reportedly low, highly sensitive and robust methods are required to detect prions in blood and blood derived products. The objectives of this study were to compare different methods--in vitro, ex vivo and in vivo assays--to detect prion infectivity in cells prepared from blood samples obtained from scrapie infected sheep at different time points of the disease. Protein misfolding cyclic amplification (PMCA) and bioassays in transgenic mice expressing the ovine prion protein were the most efficient methods to identify infected animals at any time of the disease (asymptomatic to terminally-ill stages). However scrapie cell and cerebellar organotypic slice culture assays designed to replicate ovine prions in culture also allowed detection of prion infectivity in blood cells from asymptomatic sheep. These findings confirm that white blood cells are appropriate targets for preclinical detection and introduce ex vivo tools to detect blood infectivity during the asymptomatic stage of the disease.
format	article
author	Sophie Halliez Emilie Jaumain Alvina Huor Jean-Yves Douet Séverine Lugan Hervé Cassard Caroline Lacroux Vincent Béringue Olivier Andréoletti Didier Vilette
author_facet	Sophie Halliez Emilie Jaumain Alvina Huor Jean-Yves Douet Séverine Lugan Hervé Cassard Caroline Lacroux Vincent Béringue Olivier Andréoletti Didier Vilette
author_sort	Sophie Halliez
title	White blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.
title_short	White blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.
title_full	White blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.
title_fullStr	White blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.
title_full_unstemmed	White blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.
title_sort	white blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/329de7c6b9af4ceaa86eb3c5f1445ec3
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White blood cell-based detection of asymptomatic scrapie infection by ex vivo assays.

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