Identification and Characterization of mRNA Biomarkers for Sodium Cyanide Exposure

Biomarkers in exposure assessment are defined as the quantifiable targets that indicate the exposure to hazardous chemicals and their resulting health effect. In this study, we aimed to identify, validate, and characterize the mRNA biomarker that can detect the exposure of sodium cyanide. To identif...

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Autores principales:	Min Kim, Seung-Cheol Jee, Soee Kim, Kyung-Hwa Hwang, Jung-Suk Sung
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biomarker sodium cyanide exposure assessment RNA-seq Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/32a1dc4393b64d5c806e38ac40945996
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spelling	oai:doaj.org-article:32a1dc4393b64d5c806e38ac409459962021-11-25T19:08:06ZIdentification and Characterization of mRNA Biomarkers for Sodium Cyanide Exposure10.3390/toxics91102882305-6304https://doaj.org/article/32a1dc4393b64d5c806e38ac409459962021-11-01T00:00:00Zhttps://www.mdpi.com/2305-6304/9/11/288https://doaj.org/toc/2305-6304Biomarkers in exposure assessment are defined as the quantifiable targets that indicate the exposure to hazardous chemicals and their resulting health effect. In this study, we aimed to identify, validate, and characterize the mRNA biomarker that can detect the exposure of sodium cyanide. To identify reliable biomarkers for sodium cyanide exposure, critical criteria were defined for candidate selection: (1) the expression level of mRNA significantly changes in response to sodium thiocyanate treatment in transcriptomics results (fold change > 2.0 or <0.50, adjusted <i>p</i>-value < 0.05); and (2) the mRNA level is significantly modulated by sodium cyanide exposure in both normal human lung cells and rat lung tissue. We identified the following mRNA biomarker candidates: <i>ADCY5</i>, <i>ANGPTL4</i>, <i>CCNG2</i>, <i>CD9</i>, <i>COL1A2</i>, <i>DACT3</i>, <i>GGCX</i>, <i>GRB14</i>, <i>H1F0</i>, <i>HSPA1A</i>, <i>MAF</i>, <i>MAT2A</i>, <i>PPP1R10</i>, and <i>PPP4C.</i> The expression levels of these candidates were commonly downregulated by sodium cyanide exposure both in vitro and in vivo. We functionally characterized the biomarkers and established the impact of sodium cyanide on transcriptomic profiles using in silico approaches. Our results suggest that the biomarkers may contribute to the regulation and degradation of the extracellular matrix, leading to a negative effect on surrounding lung cells.Min KimSeung-Cheol JeeSoee KimKyung-Hwa HwangJung-Suk SungMDPI AGarticlebiomarkersodium cyanideexposure assessmentRNA-seqChemical technologyTP1-1185ENToxics, Vol 9, Iss 288, p 288 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biomarker sodium cyanide exposure assessment RNA-seq Chemical technology TP1-1185
spellingShingle	biomarker sodium cyanide exposure assessment RNA-seq Chemical technology TP1-1185 Min Kim Seung-Cheol Jee Soee Kim Kyung-Hwa Hwang Jung-Suk Sung Identification and Characterization of mRNA Biomarkers for Sodium Cyanide Exposure
description	Biomarkers in exposure assessment are defined as the quantifiable targets that indicate the exposure to hazardous chemicals and their resulting health effect. In this study, we aimed to identify, validate, and characterize the mRNA biomarker that can detect the exposure of sodium cyanide. To identify reliable biomarkers for sodium cyanide exposure, critical criteria were defined for candidate selection: (1) the expression level of mRNA significantly changes in response to sodium thiocyanate treatment in transcriptomics results (fold change > 2.0 or <0.50, adjusted <i>p</i>-value < 0.05); and (2) the mRNA level is significantly modulated by sodium cyanide exposure in both normal human lung cells and rat lung tissue. We identified the following mRNA biomarker candidates: <i>ADCY5</i>, <i>ANGPTL4</i>, <i>CCNG2</i>, <i>CD9</i>, <i>COL1A2</i>, <i>DACT3</i>, <i>GGCX</i>, <i>GRB14</i>, <i>H1F0</i>, <i>HSPA1A</i>, <i>MAF</i>, <i>MAT2A</i>, <i>PPP1R10</i>, and <i>PPP4C.</i> The expression levels of these candidates were commonly downregulated by sodium cyanide exposure both in vitro and in vivo. We functionally characterized the biomarkers and established the impact of sodium cyanide on transcriptomic profiles using in silico approaches. Our results suggest that the biomarkers may contribute to the regulation and degradation of the extracellular matrix, leading to a negative effect on surrounding lung cells.
format	article
author	Min Kim Seung-Cheol Jee Soee Kim Kyung-Hwa Hwang Jung-Suk Sung
author_facet	Min Kim Seung-Cheol Jee Soee Kim Kyung-Hwa Hwang Jung-Suk Sung
author_sort	Min Kim
title	Identification and Characterization of mRNA Biomarkers for Sodium Cyanide Exposure
title_short	Identification and Characterization of mRNA Biomarkers for Sodium Cyanide Exposure
title_full	Identification and Characterization of mRNA Biomarkers for Sodium Cyanide Exposure
title_fullStr	Identification and Characterization of mRNA Biomarkers for Sodium Cyanide Exposure
title_full_unstemmed	Identification and Characterization of mRNA Biomarkers for Sodium Cyanide Exposure
title_sort	identification and characterization of mrna biomarkers for sodium cyanide exposure
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/32a1dc4393b64d5c806e38ac40945996
work_keys_str_mv	AT minkim identificationandcharacterizationofmrnabiomarkersforsodiumcyanideexposure AT seungcheoljee identificationandcharacterizationofmrnabiomarkersforsodiumcyanideexposure AT soeekim identificationandcharacterizationofmrnabiomarkersforsodiumcyanideexposure AT kyunghwahwang identificationandcharacterizationofmrnabiomarkersforsodiumcyanideexposure AT jungsuksung identificationandcharacterizationofmrnabiomarkersforsodiumcyanideexposure
_version_	1718410269639573504

Identification and Characterization of mRNA Biomarkers for Sodium Cyanide Exposure

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