Aptamer-Conjugated Gold Nanoparticles Targeting Epidermal Growth Factor Receptor Variant III for the Treatment of Glioblastoma
Li Peng,1,2,* Yanling Liang,1,* Xinxin Zhong,1 Zhiman Liang,1,2 Yinghong Tian,3 Shuji Li,1 Jingxue Liang,4 Ransheng Wang,4 Yuqi Zhong,4 Yusheng Shi,5 Xingmei Zhang1 1Key Laboratory of Mental Health of the Ministry of Education, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and...
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| Autores principales: | , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2020
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/32a525da4321468fa135cb22b593f2fb |
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| Sumario: | Li Peng,1,2,* Yanling Liang,1,* Xinxin Zhong,1 Zhiman Liang,1,2 Yinghong Tian,3 Shuji Li,1 Jingxue Liang,4 Ransheng Wang,4 Yuqi Zhong,4 Yusheng Shi,5 Xingmei Zhang1 1Key Laboratory of Mental Health of the Ministry of Education, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Guangdong Province Key Laboratory of Psychiatric Disorders, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People’s Republic of China; 2The Fifth Affiliated Hospital, Southern Medical University, Guangzhou 510900, People’s Republic of China; 3Experiment Teaching & Administration Center, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People’s Republic of China; 4The First Affiliated Hospital, Southern Medical University, Guangzhou 510515, People’s Republic of China; 5Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xingmei ZhangKey Laboratory of Mental Health of the Ministry of Education, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Guangdong Province Key Laboratory of Psychiatric Disorders, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, No. 1838 Guangzhou Avenue, Guangzhou 510515, People’s Republic of ChinaEmail zxmray@hotmail.comYusheng ShiDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Avenue, Guangzhou 510515, People’s Republic of ChinaEmail syszxm@hotmail.comPurpose: In this study, we constructed novel brain-targeting complexes (U2-AuNP) by conjugating aptamer U2 to the gold nanoparticle (AuNPs) surface as a promising option for GBM therapy.Materials and Methods: The properties of the U2-AuNP complexes were thoroughly characterized. Then, we detected the in vitro effects of U2-AuNP in U87-EGFRvIII cell lines and the in vivo antitumor effects of U2-AuNP in GBM-bearing mice. Furthermore, we explored the inhibition mechanism of U2-AuNP in U87-EGFRvIII cell lines.Results: We found that U2-AuNP inhibits the proliferation and invasion of U87-EGFRvIII cell lines and prolongs the survival time of GBM-bearing mice. We found that U2-AuNP can inhibit the EGFR-related pathway and prevent DNA damage repair in GBM cells.Conclusion: These results reveal the promising potential of U2-AuNP as a drug candidate for targeted therapy in GBM.Keywords: SELEX, EGFRvIII, GBM, therapy |
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