Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues

Super-resolution imaging of multiple target proteins in the same sample can provide important information of cellular nanostructure, but has not been routinely achieved. Here, the authors present a fully automated 3D STORM approach using a re-staining protocol to image 15 targets in single cells and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maja Klevanski, Frank Herrmannsdoerfer, Steffen Sass, Varun Venkataramani, Mike Heilemann, Thomas Kuner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/32a771440bc44ff9ab58e746e4a6a5a5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:32a771440bc44ff9ab58e746e4a6a5a5
record_format	dspace
spelling	oai:doaj.org-article:32a771440bc44ff9ab58e746e4a6a5a52021-12-02T14:40:41ZAutomated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues10.1038/s41467-020-15362-12041-1723https://doaj.org/article/32a771440bc44ff9ab58e746e4a6a5a52020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15362-1https://doaj.org/toc/2041-1723Super-resolution imaging of multiple target proteins in the same sample can provide important information of cellular nanostructure, but has not been routinely achieved. Here, the authors present a fully automated 3D STORM approach using a re-staining protocol to image 15 targets in single cells and 16 targets in neuronal tissue.Maja KlevanskiFrank HerrmannsdoerferSteffen SassVarun VenkataramaniMike HeilemannThomas KunerNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Maja Klevanski Frank Herrmannsdoerfer Steffen Sass Varun Venkataramani Mike Heilemann Thomas Kuner Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
description	Super-resolution imaging of multiple target proteins in the same sample can provide important information of cellular nanostructure, but has not been routinely achieved. Here, the authors present a fully automated 3D STORM approach using a re-staining protocol to image 15 targets in single cells and 16 targets in neuronal tissue.
format	article
author	Maja Klevanski Frank Herrmannsdoerfer Steffen Sass Varun Venkataramani Mike Heilemann Thomas Kuner
author_facet	Maja Klevanski Frank Herrmannsdoerfer Steffen Sass Varun Venkataramani Mike Heilemann Thomas Kuner
author_sort	Maja Klevanski
title	Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_short	Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_full	Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_fullStr	Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_full_unstemmed	Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_sort	automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/32a771440bc44ff9ab58e746e4a6a5a5
work_keys_str_mv	AT majaklevanski automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues AT frankherrmannsdoerfer automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues AT steffensass automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues AT varunvenkataramani automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues AT mikeheilemann automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues AT thomaskuner automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues
_version_	1718390166266052608

Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues

Ejemplares similares