In planta proximity dependent biotin identification (BioID)

Abstract Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we de...

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Main Authors:	Madiha Khan, Ji-Young Youn, Anne-Claude Gingras, Rajagopal Subramaniam, Darrell Desveaux
Format:	article
Language:	EN
Published:	Nature Portfolio 2018
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/32b6964d1e40413e9cff5ccd1cdc176d
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spelling	oai:doaj.org-article:32b6964d1e40413e9cff5ccd1cdc176d2021-12-02T15:08:44ZIn planta proximity dependent biotin identification (BioID)10.1038/s41598-018-27500-32045-2322https://doaj.org/article/32b6964d1e40413e9cff5ccd1cdc176d2018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-27500-3https://doaj.org/toc/2045-2322Abstract Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.Madiha KhanJi-Young YounAnne-Claude GingrasRajagopal SubramaniamDarrell DesveauxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Madiha Khan Ji-Young Youn Anne-Claude Gingras Rajagopal Subramaniam Darrell Desveaux In planta proximity dependent biotin identification (BioID)
description	Abstract Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.
format	article
author	Madiha Khan Ji-Young Youn Anne-Claude Gingras Rajagopal Subramaniam Darrell Desveaux
author_facet	Madiha Khan Ji-Young Youn Anne-Claude Gingras Rajagopal Subramaniam Darrell Desveaux
author_sort	Madiha Khan
title	In planta proximity dependent biotin identification (BioID)
title_short	In planta proximity dependent biotin identification (BioID)
title_full	In planta proximity dependent biotin identification (BioID)
title_fullStr	In planta proximity dependent biotin identification (BioID)
title_full_unstemmed	In planta proximity dependent biotin identification (BioID)
title_sort	in planta proximity dependent biotin identification (bioid)
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/32b6964d1e40413e9cff5ccd1cdc176d
work_keys_str_mv	AT madihakhan inplantaproximitydependentbiotinidentificationbioid AT jiyoungyoun inplantaproximitydependentbiotinidentificationbioid AT anneclaudegingras inplantaproximitydependentbiotinidentificationbioid AT rajagopalsubramaniam inplantaproximitydependentbiotinidentificationbioid AT darrelldesveaux inplantaproximitydependentbiotinidentificationbioid
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In planta proximity dependent biotin identification (BioID)

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