Identification and characterization of novel microRNAs from Schistosoma japonicum.

<h4>Background</h4>Schistosomiasis japonica remains a major public health problem in China. Its pathogen, Schistosoma japonicum has a complex life cycle and a unique repertoire of genes expressed at different life cycle stages. Exploring schistosome gene regulation will yield the best pr...

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Autores principales:	Xiangyang Xue, Jun Sun, Qingfeng Zhang, Zhangxun Wang, Yufu Huang, Weiqing Pan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/32c731f385f44935a081e781dea2feb4
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spelling	oai:doaj.org-article:32c731f385f44935a081e781dea2feb42021-11-25T06:18:02ZIdentification and characterization of novel microRNAs from Schistosoma japonicum.1932-620310.1371/journal.pone.0004034https://doaj.org/article/32c731f385f44935a081e781dea2feb42008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19107204/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Schistosomiasis japonica remains a major public health problem in China. Its pathogen, Schistosoma japonicum has a complex life cycle and a unique repertoire of genes expressed at different life cycle stages. Exploring schistosome gene regulation will yield the best prospects for new drug targets and vaccine candidates. MicroRNAs (miRNAs) are a highly conserved class of noncoding RNA that control many biological processes by sequence-specific inhibition of gene expression. Although a large number of miRNAs have been identified from plants to mammals, it remains no experimental proof whether schistosome exist miRNAs.<h4>Methodology and results</h4>We have identified novel miRNAs from Schistosoma japonicum by cloning and sequencing a small (18-26 nt) RNA cDNA library from the adult worms. Five novel miRNAs were identified from 227 cloned RNA sequences and verified by Northern blot. Alignments of the miRNAs with corresponding family members indicated that four of them belong to a metazoan miRNA family: let-7, miR-71, bantam and miR-125. The fifth potentially new (non conserved) miRNA appears to belong to a previously undescribed family in the genus Schistosome. The novel miRNAs were designated as sja-let-7, sja-miR-71, sja-bantam, sja-miR-125 and sja-miR-new1, respectively. Expression of sja-let-7, sja-miR-71 and sja-bantam were analyzed in six stages of the life cycle, i.e. egg, miracidium, sporocyst, cercaria, schistosomulum, and adult worm, by a modified stem-loop reverse transcribed polymerase chain reaction (RT-PCR) method developed in our laboratory. The expression patterns of these miRNAs were highly stage-specific. In particular, sja-miR-71 and sja-bantam expression reach their peaks in the cercaria stage and then drop quickly to the nadirs in the schistosomulum stage, following penetration of cercaria into a mammalian host.<h4>Conclusions</h4>Authentic miRNAs were identified for the first time in S. japonicum, including a new schistosome family member. The different expression patterns of the novel miRNAs over the life stages of S. japonicum suggest that they may mediate important roles in Schistosome growth and development.Xiangyang XueJun SunQingfeng ZhangZhangxun WangYufu HuangWeiqing PanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e4034 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiangyang Xue Jun Sun Qingfeng Zhang Zhangxun Wang Yufu Huang Weiqing Pan Identification and characterization of novel microRNAs from Schistosoma japonicum.
description	<h4>Background</h4>Schistosomiasis japonica remains a major public health problem in China. Its pathogen, Schistosoma japonicum has a complex life cycle and a unique repertoire of genes expressed at different life cycle stages. Exploring schistosome gene regulation will yield the best prospects for new drug targets and vaccine candidates. MicroRNAs (miRNAs) are a highly conserved class of noncoding RNA that control many biological processes by sequence-specific inhibition of gene expression. Although a large number of miRNAs have been identified from plants to mammals, it remains no experimental proof whether schistosome exist miRNAs.<h4>Methodology and results</h4>We have identified novel miRNAs from Schistosoma japonicum by cloning and sequencing a small (18-26 nt) RNA cDNA library from the adult worms. Five novel miRNAs were identified from 227 cloned RNA sequences and verified by Northern blot. Alignments of the miRNAs with corresponding family members indicated that four of them belong to a metazoan miRNA family: let-7, miR-71, bantam and miR-125. The fifth potentially new (non conserved) miRNA appears to belong to a previously undescribed family in the genus Schistosome. The novel miRNAs were designated as sja-let-7, sja-miR-71, sja-bantam, sja-miR-125 and sja-miR-new1, respectively. Expression of sja-let-7, sja-miR-71 and sja-bantam were analyzed in six stages of the life cycle, i.e. egg, miracidium, sporocyst, cercaria, schistosomulum, and adult worm, by a modified stem-loop reverse transcribed polymerase chain reaction (RT-PCR) method developed in our laboratory. The expression patterns of these miRNAs were highly stage-specific. In particular, sja-miR-71 and sja-bantam expression reach their peaks in the cercaria stage and then drop quickly to the nadirs in the schistosomulum stage, following penetration of cercaria into a mammalian host.<h4>Conclusions</h4>Authentic miRNAs were identified for the first time in S. japonicum, including a new schistosome family member. The different expression patterns of the novel miRNAs over the life stages of S. japonicum suggest that they may mediate important roles in Schistosome growth and development.
format	article
author	Xiangyang Xue Jun Sun Qingfeng Zhang Zhangxun Wang Yufu Huang Weiqing Pan
author_facet	Xiangyang Xue Jun Sun Qingfeng Zhang Zhangxun Wang Yufu Huang Weiqing Pan
author_sort	Xiangyang Xue
title	Identification and characterization of novel microRNAs from Schistosoma japonicum.
title_short	Identification and characterization of novel microRNAs from Schistosoma japonicum.
title_full	Identification and characterization of novel microRNAs from Schistosoma japonicum.
title_fullStr	Identification and characterization of novel microRNAs from Schistosoma japonicum.
title_full_unstemmed	Identification and characterization of novel microRNAs from Schistosoma japonicum.
title_sort	identification and characterization of novel micrornas from schistosoma japonicum.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/32c731f385f44935a081e781dea2feb4
work_keys_str_mv	AT xiangyangxue identificationandcharacterizationofnovelmicrornasfromschistosomajaponicum AT junsun identificationandcharacterizationofnovelmicrornasfromschistosomajaponicum AT qingfengzhang identificationandcharacterizationofnovelmicrornasfromschistosomajaponicum AT zhangxunwang identificationandcharacterizationofnovelmicrornasfromschistosomajaponicum AT yufuhuang identificationandcharacterizationofnovelmicrornasfromschistosomajaponicum AT weiqingpan identificationandcharacterizationofnovelmicrornasfromschistosomajaponicum
_version_	1718413915834023936

Identification and characterization of novel microRNAs from Schistosoma japonicum.

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