Characterization of a novel two-component Na+(Li+, K+)/H+ antiporter from Halomonas zhaodongensis
Abstract In this study, genomic DNA was screened for novel Na+/H+ antiporter genes from Halomonas zhaodongensis by selection in Escherichia coli KNabc lacking three major Na+/H+ antiporters. Co-expression of two genes designated umpAB, encoding paired homologous unknown membrane proteins belonging t...
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2017
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oai:doaj.org-article:32c8642356134fc0829b6701eb581ed32021-12-02T15:05:08ZCharacterization of a novel two-component Na+(Li+, K+)/H+ antiporter from Halomonas zhaodongensis10.1038/s41598-017-04236-02045-2322https://doaj.org/article/32c8642356134fc0829b6701eb581ed32017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04236-0https://doaj.org/toc/2045-2322Abstract In this study, genomic DNA was screened for novel Na+/H+ antiporter genes from Halomonas zhaodongensis by selection in Escherichia coli KNabc lacking three major Na+/H+ antiporters. Co-expression of two genes designated umpAB, encoding paired homologous unknown membrane proteins belonging to DUF1538 (domain of unknown function with No. 1538) family, were found to confer E. coli KNabc the tolerance to 0.4 M NaCl and 30 mM LiCl, and an alkaline pH resistance at 8.0. Western blot and co-immunoprecipitation establish that UmpAB localize as a hetero-dimer in the cytoplasmic membranes. Functional analysis reveals that UmpAB exhibit pH-dependent Na+(Li+, K+)/H+ antiport activity at a wide pH range of 6.5 to 9.5 with an optimal pH at 9.0. Neither UmpA nor UmpB showed homology with known single-gene or multi-gene Na+/H+ antiporters, or such proteins as ChaA, MdfA, TetA(L), Nap and PsmrAB with Na+/H+ antiport activity. Phylogenetic analysis confirms that UmpAB should belong to DUF1538 family, which are significantly distant with the above-mentioned proteins with Na+/H+ antiport activity. Taken together, we propose that UmpAB represent a novel two-component Na+(Li+, K+)/H+ antiporter. To the best of our knowledge, this is the first report on the functional analysis of unknown membrane proteins belonging to DUF1538 family.Lin MengFankui MengRui ZhangZhenglai ZhangPing DongKaifu SunJin ChenWei ZhangMingxue YanJincheng LiHeba Abdel-motaalJuquan JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) |
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Medicine R Science Q Lin Meng Fankui Meng Rui Zhang Zhenglai Zhang Ping Dong Kaifu Sun Jin Chen Wei Zhang Mingxue Yan Jincheng Li Heba Abdel-motaal Juquan Jiang Characterization of a novel two-component Na+(Li+, K+)/H+ antiporter from Halomonas zhaodongensis |
| description |
Abstract In this study, genomic DNA was screened for novel Na+/H+ antiporter genes from Halomonas zhaodongensis by selection in Escherichia coli KNabc lacking three major Na+/H+ antiporters. Co-expression of two genes designated umpAB, encoding paired homologous unknown membrane proteins belonging to DUF1538 (domain of unknown function with No. 1538) family, were found to confer E. coli KNabc the tolerance to 0.4 M NaCl and 30 mM LiCl, and an alkaline pH resistance at 8.0. Western blot and co-immunoprecipitation establish that UmpAB localize as a hetero-dimer in the cytoplasmic membranes. Functional analysis reveals that UmpAB exhibit pH-dependent Na+(Li+, K+)/H+ antiport activity at a wide pH range of 6.5 to 9.5 with an optimal pH at 9.0. Neither UmpA nor UmpB showed homology with known single-gene or multi-gene Na+/H+ antiporters, or such proteins as ChaA, MdfA, TetA(L), Nap and PsmrAB with Na+/H+ antiport activity. Phylogenetic analysis confirms that UmpAB should belong to DUF1538 family, which are significantly distant with the above-mentioned proteins with Na+/H+ antiport activity. Taken together, we propose that UmpAB represent a novel two-component Na+(Li+, K+)/H+ antiporter. To the best of our knowledge, this is the first report on the functional analysis of unknown membrane proteins belonging to DUF1538 family. |
| format |
article |
| author |
Lin Meng Fankui Meng Rui Zhang Zhenglai Zhang Ping Dong Kaifu Sun Jin Chen Wei Zhang Mingxue Yan Jincheng Li Heba Abdel-motaal Juquan Jiang |
| author_facet |
Lin Meng Fankui Meng Rui Zhang Zhenglai Zhang Ping Dong Kaifu Sun Jin Chen Wei Zhang Mingxue Yan Jincheng Li Heba Abdel-motaal Juquan Jiang |
| author_sort |
Lin Meng |
| title |
Characterization of a novel two-component Na+(Li+, K+)/H+ antiporter from Halomonas zhaodongensis |
| title_short |
Characterization of a novel two-component Na+(Li+, K+)/H+ antiporter from Halomonas zhaodongensis |
| title_full |
Characterization of a novel two-component Na+(Li+, K+)/H+ antiporter from Halomonas zhaodongensis |
| title_fullStr |
Characterization of a novel two-component Na+(Li+, K+)/H+ antiporter from Halomonas zhaodongensis |
| title_full_unstemmed |
Characterization of a novel two-component Na+(Li+, K+)/H+ antiporter from Halomonas zhaodongensis |
| title_sort |
characterization of a novel two-component na+(li+, k+)/h+ antiporter from halomonas zhaodongensis |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/32c8642356134fc0829b6701eb581ed3 |
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