Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit

Objective To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences. Methods Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were...

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Autores principales:	Sheng Huang, Kangli Xu, Shanshan Xu, Shijun Yu, Yan Miao, Juan Wang
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/32c909ce4cba409787ddd7a4140e7133
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spelling	oai:doaj.org-article:32c909ce4cba409787ddd7a4140e71332021-11-15T00:03:47ZEnumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit1473-230010.1177/03000605211055384https://doaj.org/article/32c909ce4cba409787ddd7a4140e71332021-11-01T00:00:00Zhttps://doi.org/10.1177/03000605211055384https://doaj.org/toc/1473-2300Objective To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences. Methods Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software. Results A total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: −5.7% to−14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: −2.1% to +13.8%). The intraclass correlation coefficients for absolute and relative counts of CD34-positive cells were 0.9967 (95% confidence interval [CI]: 0.9961–0.9972) and 0.9512 (95% CI: 0.9423–0.9587) for the BD Biosciences and Beckman Coulter kits, respectively. Conclusions The hematopoietic stem cell enumeration kit developed by BD Biosciences can be used to enumerate CD34-positive stem cells from cord blood samples.Sheng HuangKangli XuShanshan XuShijun YuYan MiaoJuan WangSAGE PublishingarticleMedicine (General)R5-920ENJournal of International Medical Research, Vol 49 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Sheng Huang Kangli Xu Shanshan Xu Shijun Yu Yan Miao Juan Wang Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit
description	Objective To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences. Methods Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software. Results A total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: −5.7% to−14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: −2.1% to +13.8%). The intraclass correlation coefficients for absolute and relative counts of CD34-positive cells were 0.9967 (95% confidence interval [CI]: 0.9961–0.9972) and 0.9512 (95% CI: 0.9423–0.9587) for the BD Biosciences and Beckman Coulter kits, respectively. Conclusions The hematopoietic stem cell enumeration kit developed by BD Biosciences can be used to enumerate CD34-positive stem cells from cord blood samples.
format	article
author	Sheng Huang Kangli Xu Shanshan Xu Shijun Yu Yan Miao Juan Wang
author_facet	Sheng Huang Kangli Xu Shanshan Xu Shijun Yu Yan Miao Juan Wang
author_sort	Sheng Huang
title	Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit
title_short	Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit
title_full	Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit
title_fullStr	Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit
title_full_unstemmed	Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit
title_sort	enumeration of viable cd34+ cells in cord blood using a novel stem cell enumeration kit
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/32c909ce4cba409787ddd7a4140e7133
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_version_	1718428974623752192

Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit

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