Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage

Abstract In multicellular organisms, oocytes and sperm undergo fusion during fertilization and the resulting zygote gives rise to a new individual. The ability of zygotes to produce a fully formed individual from a single cell when placed in a supportive environment is known as totipotency. Given th...

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Autores principales:	Marino Maemura, Hiroaki Taketsuru, Yuki Nakajima, Ruiqi Shao, Ayaka Kakihara, Jumpei Nogami, Yasuyuki Ohkawa, Yu-ichi Tsukada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/32d8f9ca35ce40d48451b0c8ff8c54cf
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spelling	oai:doaj.org-article:32d8f9ca35ce40d48451b0c8ff8c54cf2021-12-02T16:53:20ZTotipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage10.1038/s41598-021-90653-12045-2322https://doaj.org/article/32d8f9ca35ce40d48451b0c8ff8c54cf2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90653-1https://doaj.org/toc/2045-2322Abstract In multicellular organisms, oocytes and sperm undergo fusion during fertilization and the resulting zygote gives rise to a new individual. The ability of zygotes to produce a fully formed individual from a single cell when placed in a supportive environment is known as totipotency. Given that totipotent cells are the source of all multicellular organisms, a better understanding of totipotency may have a wide-ranging impact on biology. The precise delineation of totipotent cells in mammals has remained elusive, however, although zygotes and single blastomeres of embryos at the two-cell stage have been thought to be the only totipotent cells in mice. We now show that a single blastomere of two- or four-cell mouse embryos can give rise to a fertile adult when placed in a uterus, even though blastomere isolation disturbs the transcriptome of derived embryos. Single blastomeres isolated from embryos at the eight-cell or morula stages and cultured in vitro manifested pronounced defects in the formation of epiblast and primitive endoderm by the inner cell mass and in the development of blastocysts, respectively. Our results thus indicate that totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage.Marino MaemuraHiroaki TaketsuruYuki NakajimaRuiqi ShaoAyaka KakiharaJumpei NogamiYasuyuki OhkawaYu-ichi TsukadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marino Maemura Hiroaki Taketsuru Yuki Nakajima Ruiqi Shao Ayaka Kakihara Jumpei Nogami Yasuyuki Ohkawa Yu-ichi Tsukada Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage
description	Abstract In multicellular organisms, oocytes and sperm undergo fusion during fertilization and the resulting zygote gives rise to a new individual. The ability of zygotes to produce a fully formed individual from a single cell when placed in a supportive environment is known as totipotency. Given that totipotent cells are the source of all multicellular organisms, a better understanding of totipotency may have a wide-ranging impact on biology. The precise delineation of totipotent cells in mammals has remained elusive, however, although zygotes and single blastomeres of embryos at the two-cell stage have been thought to be the only totipotent cells in mice. We now show that a single blastomere of two- or four-cell mouse embryos can give rise to a fertile adult when placed in a uterus, even though blastomere isolation disturbs the transcriptome of derived embryos. Single blastomeres isolated from embryos at the eight-cell or morula stages and cultured in vitro manifested pronounced defects in the formation of epiblast and primitive endoderm by the inner cell mass and in the development of blastocysts, respectively. Our results thus indicate that totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage.
format	article
author	Marino Maemura Hiroaki Taketsuru Yuki Nakajima Ruiqi Shao Ayaka Kakihara Jumpei Nogami Yasuyuki Ohkawa Yu-ichi Tsukada
author_facet	Marino Maemura Hiroaki Taketsuru Yuki Nakajima Ruiqi Shao Ayaka Kakihara Jumpei Nogami Yasuyuki Ohkawa Yu-ichi Tsukada
author_sort	Marino Maemura
title	Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage
title_short	Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage
title_full	Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage
title_fullStr	Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage
title_full_unstemmed	Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage
title_sort	totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/32d8f9ca35ce40d48451b0c8ff8c54cf
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Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage

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