In vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken.

A total number of 16 Escherichia coli serovars were recovered from different colibacillosis diseased chicken organs. Characterization of the isolates was performed by biochemical and serological tests, biofilm formation, antimicrobial susceptibility assays, detection of virulence and antibiotic resi...

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Autores principales:	Rokaya Elamary, Dina Shibat El-hamed, Waeil Sayed, Wesam Mohamed Ali Salem
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2018
Materias:	Allium sativum Antibiotic-resistant genes Biofilm Virulence genes Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/32d99d233381418e9b5fa7c5228a0985
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spelling	oai:doaj.org-article:32d99d233381418e9b5fa7c5228a09852021-12-02T02:53:38ZIn vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken. 2535-18262535-1877https://doaj.org/article/32d99d233381418e9b5fa7c5228a09852018-11-01T00:00:00Zhttps://portal.svu.edu.eg/svu-src/index.php/ijvs/article/view/35/42https://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877A total number of 16 Escherichia coli serovars were recovered from different colibacillosis diseased chicken organs. Characterization of the isolates was performed by biochemical and serological tests, biofilm formation, antimicrobial susceptibility assays, detection of virulence and antibiotic resistance genes by PCR. The biofilm formation (at OD595) of all E. coli isolates showed a similar trend. eaeA gene was detected in 3 serovars O2:H6, O26:H11 and O44:H18 while, hly gene was detected in 2 serovars O2:H6 and O144:H21. The gene encoding for stx2 was detected in 2 serovars (O55:H7 and O146:H21) while the gene encoding stx1 was not detected in any serovars. Furthermore, all serovars were carrying the encoding blaTEM. blaSHV and blaCTX were detected in 75% and 18.75% of isolates respectively, while blaOXA-1 was not detected in all serovars. Although all isolates were multi-resistant against most of B-lactams antibiotics, they were susceptible to 20 to 80 mg ml-1 of garlic extract. Finally, we demonstrated that a five-oral administration of garlic extract to baby chicks colonized with E. coli reduces the mortalities and significantly increase the body weight. The efficacy of garlic extract as the antibacterial natural product was sufficient as a bactericidal alternative against the multidrug- resistant E. coli isolates.Rokaya Elamary Dina Shibat El-hamed Waeil Sayed Wesam Mohamed Ali Salem South Valley UniversityarticleAllium sativumAntibiotic-resistant genesBiofilmVirulence genesAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 1, Iss 2, Pp 33-49 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Allium sativum Antibiotic-resistant genes Biofilm Virulence genes Agriculture S Veterinary medicine SF600-1100
spellingShingle	Allium sativum Antibiotic-resistant genes Biofilm Virulence genes Agriculture S Veterinary medicine SF600-1100 Rokaya Elamary Dina Shibat El-hamed Waeil Sayed Wesam Mohamed Ali Salem In vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken.
description	A total number of 16 Escherichia coli serovars were recovered from different colibacillosis diseased chicken organs. Characterization of the isolates was performed by biochemical and serological tests, biofilm formation, antimicrobial susceptibility assays, detection of virulence and antibiotic resistance genes by PCR. The biofilm formation (at OD595) of all E. coli isolates showed a similar trend. eaeA gene was detected in 3 serovars O2:H6, O26:H11 and O44:H18 while, hly gene was detected in 2 serovars O2:H6 and O144:H21. The gene encoding for stx2 was detected in 2 serovars (O55:H7 and O146:H21) while the gene encoding stx1 was not detected in any serovars. Furthermore, all serovars were carrying the encoding blaTEM. blaSHV and blaCTX were detected in 75% and 18.75% of isolates respectively, while blaOXA-1 was not detected in all serovars. Although all isolates were multi-resistant against most of B-lactams antibiotics, they were susceptible to 20 to 80 mg ml-1 of garlic extract. Finally, we demonstrated that a five-oral administration of garlic extract to baby chicks colonized with E. coli reduces the mortalities and significantly increase the body weight. The efficacy of garlic extract as the antibacterial natural product was sufficient as a bactericidal alternative against the multidrug- resistant E. coli isolates.
format	article
author	Rokaya Elamary Dina Shibat El-hamed Waeil Sayed Wesam Mohamed Ali Salem
author_facet	Rokaya Elamary Dina Shibat El-hamed Waeil Sayed Wesam Mohamed Ali Salem
author_sort	Rokaya Elamary
title	In vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken.
title_short	In vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken.
title_full	In vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken.
title_fullStr	In vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken.
title_full_unstemmed	In vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken.
title_sort	in vitro and in vivo molecular studies on some escherichia coli serovars isolated from chicken.
publisher	South Valley University
publishDate	2018
url	https://doaj.org/article/32d99d233381418e9b5fa7c5228a0985
work_keys_str_mv	AT rokayaelamary invitroandinvivomolecularstudiesonsomeescherichiacoliserovarsisolatedfromchicken AT dinashibatelhamed invitroandinvivomolecularstudiesonsomeescherichiacoliserovarsisolatedfromchicken AT waeilsayed invitroandinvivomolecularstudiesonsomeescherichiacoliserovarsisolatedfromchicken AT wesammohamedalisalem invitroandinvivomolecularstudiesonsomeescherichiacoliserovarsisolatedfromchicken
_version_	1718402031832530944

In vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken.

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