pH-sensitive Au–BSA–DOX–FA nanocomposites for combined CT imaging and targeted drug delivery
He Huang,1 Da-Peng Yang,2 Minghuan Liu,2 Xiangsheng Wang,1 Zhiyong Zhang,1 Guangdong Zhou,1 Wei Liu,1 Yilin Cao,1 Wen Jie Zhang,1 Xiansong Wang1 1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai...
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Dove Medical Press
2017
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oai:doaj.org-article:32dc6ae55fcd4ad4b7aeb183bb36e74d2021-12-02T01:45:10ZpH-sensitive Au–BSA–DOX–FA nanocomposites for combined CT imaging and targeted drug delivery1178-2013https://doaj.org/article/32dc6ae55fcd4ad4b7aeb183bb36e74d2017-04-01T00:00:00Zhttps://www.dovepress.com/ph-sensitive-aundashbsandashdoxndashfa-nanocomposites-for-combined-ct--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013He Huang,1 Da-Peng Yang,2 Minghuan Liu,2 Xiangsheng Wang,1 Zhiyong Zhang,1 Guangdong Zhou,1 Wei Liu,1 Yilin Cao,1 Wen Jie Zhang,1 Xiansong Wang1 1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai, 2College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou, People’s Republic of China Abstract: Albumin-based nanoparticles (NPs) as a drug delivery system have attracted much attention owing to their nontoxicity, non-immunogenicity, great stability and ability to bind to many therapeutic drugs. Herein, bovine serum albumin (BSA) was utilized as a template to prepare Au–BSA core/shell NPs. The outer layer BSA was subsequently conjugated with cis-aconityl doxorubicin (DOX) and folic acid (FA) to create Au–BSA–DOX–FA nanocomposites. A list of characterizations was undertaken to identify the successful conjugation of drug molecules and targeted agents. In vitro cytotoxicity using a cell counting kit-8 (CCK-8) assay indicated that Au–BSA NPs did not display obvious cytotoxicity to MGC-803 and GES-1 cells in the concentration range of 0–100 µg/mL, which can therefore be used as a safe drug delivery carrier. Furthermore, compared with free DOX, Au–BSA–DOX–FA nanocomposites exhibited a pH-sensitive drug release ability and superior antitumor activity in a drug concentration-dependent manner. In vivo computed tomography (CT) imaging experiments showed that Au–BSA–DOX–FA nanocomposites could be used as an efficient and durable CT contrast agent for targeted CT imaging of the folate receptor (FR) overexpressed in cancer tissues. In vivo antitumor experiments demonstrated that Au–BSA–DOX–FA nanocomposites have selective antitumor activity effects on FR-overexpressing tumors and no adverse effects on normal tissues and organs. In conclusion, the Au–BSA–DOX–FA nanocomposite exhibits selective targeting activity, X-ray attenuation activity and pH-sensitive drug release activity. Therefore, it can enhance CT imaging and improve the targeting therapeutic efficacy of FR-overexpressing gastric cancers. Our findings suggest that Au–BSA–DOX–FA nanocomposite is a novel drug delivery carrier and a promising candidate for cancer theranostic applications. Keywords: gold nanoparticles, bovine serum albumin, CT imaging, drug delivery, theranosticsHuang HYang DLiu MWang XZhang ZZhou GLiu WCao YZhang WJWang XDove Medical Pressarticlegold nanoparticlesbovine serum albuminCT imagingdrug deliverytheranosticsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2829-2843 (2017) |
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gold nanoparticles bovine serum albumin CT imaging drug delivery theranostics Medicine (General) R5-920 |
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gold nanoparticles bovine serum albumin CT imaging drug delivery theranostics Medicine (General) R5-920 Huang H Yang D Liu M Wang X Zhang Z Zhou G Liu W Cao Y Zhang WJ Wang X pH-sensitive Au–BSA–DOX–FA nanocomposites for combined CT imaging and targeted drug delivery |
| description |
He Huang,1 Da-Peng Yang,2 Minghuan Liu,2 Xiangsheng Wang,1 Zhiyong Zhang,1 Guangdong Zhou,1 Wei Liu,1 Yilin Cao,1 Wen Jie Zhang,1 Xiansong Wang1 1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai, 2College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou, People’s Republic of China Abstract: Albumin-based nanoparticles (NPs) as a drug delivery system have attracted much attention owing to their nontoxicity, non-immunogenicity, great stability and ability to bind to many therapeutic drugs. Herein, bovine serum albumin (BSA) was utilized as a template to prepare Au–BSA core/shell NPs. The outer layer BSA was subsequently conjugated with cis-aconityl doxorubicin (DOX) and folic acid (FA) to create Au–BSA–DOX–FA nanocomposites. A list of characterizations was undertaken to identify the successful conjugation of drug molecules and targeted agents. In vitro cytotoxicity using a cell counting kit-8 (CCK-8) assay indicated that Au–BSA NPs did not display obvious cytotoxicity to MGC-803 and GES-1 cells in the concentration range of 0–100 µg/mL, which can therefore be used as a safe drug delivery carrier. Furthermore, compared with free DOX, Au–BSA–DOX–FA nanocomposites exhibited a pH-sensitive drug release ability and superior antitumor activity in a drug concentration-dependent manner. In vivo computed tomography (CT) imaging experiments showed that Au–BSA–DOX–FA nanocomposites could be used as an efficient and durable CT contrast agent for targeted CT imaging of the folate receptor (FR) overexpressed in cancer tissues. In vivo antitumor experiments demonstrated that Au–BSA–DOX–FA nanocomposites have selective antitumor activity effects on FR-overexpressing tumors and no adverse effects on normal tissues and organs. In conclusion, the Au–BSA–DOX–FA nanocomposite exhibits selective targeting activity, X-ray attenuation activity and pH-sensitive drug release activity. Therefore, it can enhance CT imaging and improve the targeting therapeutic efficacy of FR-overexpressing gastric cancers. Our findings suggest that Au–BSA–DOX–FA nanocomposite is a novel drug delivery carrier and a promising candidate for cancer theranostic applications. Keywords: gold nanoparticles, bovine serum albumin, CT imaging, drug delivery, theranostics |
| format |
article |
| author |
Huang H Yang D Liu M Wang X Zhang Z Zhou G Liu W Cao Y Zhang WJ Wang X |
| author_facet |
Huang H Yang D Liu M Wang X Zhang Z Zhou G Liu W Cao Y Zhang WJ Wang X |
| author_sort |
Huang H |
| title |
pH-sensitive Au–BSA–DOX–FA nanocomposites for combined CT imaging and targeted drug delivery |
| title_short |
pH-sensitive Au–BSA–DOX–FA nanocomposites for combined CT imaging and targeted drug delivery |
| title_full |
pH-sensitive Au–BSA–DOX–FA nanocomposites for combined CT imaging and targeted drug delivery |
| title_fullStr |
pH-sensitive Au–BSA–DOX–FA nanocomposites for combined CT imaging and targeted drug delivery |
| title_full_unstemmed |
pH-sensitive Au–BSA–DOX–FA nanocomposites for combined CT imaging and targeted drug delivery |
| title_sort |
ph-sensitive au–bsa–dox–fa nanocomposites for combined ct imaging and targeted drug delivery |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/32dc6ae55fcd4ad4b7aeb183bb36e74d |
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