Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration

Abstract Acute kidney injury (AKI) is a public health problem worldwide. Several therapeutic strategies have been made to accelerate recovery and improve renal survival. Recent studies have shown that human adult renal progenitor cells (ARPCs) participate in kidney repair processes, and may be used...

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Autores principales:	Fabio Sallustio, Claudia Curci, Alessandra Aloisi, Chiara Cristina Toma, Elisabetta Marulli, Grazia Serino, Sharon Natasha Cox, Giuseppe De Palma, Alessandra Stasi, Chiara Divella, Rosaria Rinaldi, Francesco Paolo Schena
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/32ddc66a042847169eeea2569b846557
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spelling	oai:doaj.org-article:32ddc66a042847169eeea2569b8465572021-12-02T11:52:24ZInhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration10.1038/s41598-017-08474-02045-2322https://doaj.org/article/32ddc66a042847169eeea2569b8465572017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08474-0https://doaj.org/toc/2045-2322Abstract Acute kidney injury (AKI) is a public health problem worldwide. Several therapeutic strategies have been made to accelerate recovery and improve renal survival. Recent studies have shown that human adult renal progenitor cells (ARPCs) participate in kidney repair processes, and may be used as a possible treatment to promote regeneration in acute kidney injury. Here, we show that human tubular ARPCs (tARPCs) protect physically injured or chemically damaged renal proximal tubular epithelial cells (RPTECs) by preventing cisplatin-induced apoptosis and enhancing proliferation of survived cells. tARPCs without toll-like receptor 2 (TLR2) expression or TLR2 blocking completely abrogated this regenerative effect. Only tARPCs, and not glomerular ARPCs, were able to induce tubular cell regeneration process and it occurred only after damage detection. Moreover, we have found that ARPCs secreted inhibin-A and decorin following the RPTEC damage and that these secreted factors were directly involved in cell regeneration process. Polysaccharide synthetic vesicles containing these molecules were constructed and co-cultured with cisplatin damaged RPTECs. These synthetic vesicles were not only incorporated into the cells, but they were also able to induce a substantial increase in cell number and viability. The findings of this study increase the knowledge of renal repair processes and may be the first step in the development of new specific therapeutic strategies for renal repair.Fabio SallustioClaudia CurciAlessandra AloisiChiara Cristina TomaElisabetta MarulliGrazia SerinoSharon Natasha CoxGiuseppe De PalmaAlessandra StasiChiara DivellaRosaria RinaldiFrancesco Paolo SchenaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabio Sallustio Claudia Curci Alessandra Aloisi Chiara Cristina Toma Elisabetta Marulli Grazia Serino Sharon Natasha Cox Giuseppe De Palma Alessandra Stasi Chiara Divella Rosaria Rinaldi Francesco Paolo Schena Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
description	Abstract Acute kidney injury (AKI) is a public health problem worldwide. Several therapeutic strategies have been made to accelerate recovery and improve renal survival. Recent studies have shown that human adult renal progenitor cells (ARPCs) participate in kidney repair processes, and may be used as a possible treatment to promote regeneration in acute kidney injury. Here, we show that human tubular ARPCs (tARPCs) protect physically injured or chemically damaged renal proximal tubular epithelial cells (RPTECs) by preventing cisplatin-induced apoptosis and enhancing proliferation of survived cells. tARPCs without toll-like receptor 2 (TLR2) expression or TLR2 blocking completely abrogated this regenerative effect. Only tARPCs, and not glomerular ARPCs, were able to induce tubular cell regeneration process and it occurred only after damage detection. Moreover, we have found that ARPCs secreted inhibin-A and decorin following the RPTEC damage and that these secreted factors were directly involved in cell regeneration process. Polysaccharide synthetic vesicles containing these molecules were constructed and co-cultured with cisplatin damaged RPTECs. These synthetic vesicles were not only incorporated into the cells, but they were also able to induce a substantial increase in cell number and viability. The findings of this study increase the knowledge of renal repair processes and may be the first step in the development of new specific therapeutic strategies for renal repair.
format	article
author	Fabio Sallustio Claudia Curci Alessandra Aloisi Chiara Cristina Toma Elisabetta Marulli Grazia Serino Sharon Natasha Cox Giuseppe De Palma Alessandra Stasi Chiara Divella Rosaria Rinaldi Francesco Paolo Schena
author_facet	Fabio Sallustio Claudia Curci Alessandra Aloisi Chiara Cristina Toma Elisabetta Marulli Grazia Serino Sharon Natasha Cox Giuseppe De Palma Alessandra Stasi Chiara Divella Rosaria Rinaldi Francesco Paolo Schena
author_sort	Fabio Sallustio
title	Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
title_short	Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
title_full	Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
title_fullStr	Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
title_full_unstemmed	Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
title_sort	inhibin-a and decorin secreted by human adult renal stem/progenitor cells through the tlr2 engagement induce renal tubular cell regeneration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/32ddc66a042847169eeea2569b846557
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Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration

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