Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.

Most fish embryos that develop externally are exposed to an environment full of microbes. How they survive microbial attacks are not understood to date. Here we demonstrated that the egg cytosol prepared from the newly fertilized eggs of zebrafish Danio rerio is capable of killing the Gram-negative...

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Autores principales:	Zhiping Wang, Shicui Zhang, Guangfeng Wang, Yan An
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/32ecb2a024604f56a1ac9896eb09b40f
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spelling	oai:doaj.org-article:32ecb2a024604f56a1ac9896eb09b40f2021-11-25T06:13:36ZComplement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.1932-620310.1371/journal.pone.0001463https://doaj.org/article/32ecb2a024604f56a1ac9896eb09b40f2008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18213372/?tool=EBIhttps://doaj.org/toc/1932-6203Most fish embryos that develop externally are exposed to an environment full of microbes. How they survive microbial attacks are not understood to date. Here we demonstrated that the egg cytosol prepared from the newly fertilized eggs of zebrafish Danio rerio is capable of killing the Gram-negative bacterium Escherichia coli, via in vitro assay system of the complement activity established. All findings indicate that it is the complement system operating via the alternative pathway that is attributable to the bacteriolytic activity. This is the first report providing the evidence for the functional role of the maternal complement components in fish eggs, paving the way for study of maternal immunity in other organisms whose eggs are fertilized in vitro.Zhiping WangShicui ZhangGuangfeng WangYan AnPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 1, p e1463 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhiping Wang Shicui Zhang Guangfeng Wang Yan An Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.
description	Most fish embryos that develop externally are exposed to an environment full of microbes. How they survive microbial attacks are not understood to date. Here we demonstrated that the egg cytosol prepared from the newly fertilized eggs of zebrafish Danio rerio is capable of killing the Gram-negative bacterium Escherichia coli, via in vitro assay system of the complement activity established. All findings indicate that it is the complement system operating via the alternative pathway that is attributable to the bacteriolytic activity. This is the first report providing the evidence for the functional role of the maternal complement components in fish eggs, paving the way for study of maternal immunity in other organisms whose eggs are fertilized in vitro.
format	article
author	Zhiping Wang Shicui Zhang Guangfeng Wang Yan An
author_facet	Zhiping Wang Shicui Zhang Guangfeng Wang Yan An
author_sort	Zhiping Wang
title	Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.
title_short	Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.
title_full	Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.
title_fullStr	Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.
title_full_unstemmed	Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.
title_sort	complement activity in the egg cytosol of zebrafish danio rerio: evidence for the defense role of maternal complement components.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/32ecb2a024604f56a1ac9896eb09b40f
work_keys_str_mv	AT zhipingwang complementactivityintheeggcytosolofzebrafishdaniorerioevidenceforthedefenseroleofmaternalcomplementcomponents AT shicuizhang complementactivityintheeggcytosolofzebrafishdaniorerioevidenceforthedefenseroleofmaternalcomplementcomponents AT guangfengwang complementactivityintheeggcytosolofzebrafishdaniorerioevidenceforthedefenseroleofmaternalcomplementcomponents AT yanan complementactivityintheeggcytosolofzebrafishdaniorerioevidenceforthedefenseroleofmaternalcomplementcomponents
_version_	1718413974509191168

Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components.

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