Gut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (MIVAS study)

Introduction Intestinal microbiota is arising as a new element in the physiopathology of cardiovascular diseases. A healthy microbiota includes a balanced representation of bacteria with health promotion functions (symbiotes). The aim of this study is to analyse the relationship between intestinal m...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luis Garcia-Ortiz, Manuel A Gómez-Marcos, Emiliano Rodriguez-Sanchez, Rita Salvado, Sandra Santos-Minguez, Cristina Agudo-Conde, Cristina Lugones-Sanchez, Angela Cabo-Laso, Jesus Mª Hernandez-Sanchez, Rocio Benito, Jesus M Hernandez-Rivas, Pedro Guimarães Cunha, MIVAS Investigators
Formato:	article
Lenguaje:	EN
Publicado:	BMJ Publishing Group 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/32f768282dd348468e9dcc478c9db59e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:32f768282dd348468e9dcc478c9db59e
record_format	dspace
spelling	oai:doaj.org-article:32f768282dd348468e9dcc478c9db59e2021-11-18T09:30:07ZGut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (MIVAS study)10.1136/bmjopen-2020-0389332044-6055https://doaj.org/article/32f768282dd348468e9dcc478c9db59e2021-02-01T00:00:00Zhttps://bmjopen.bmj.com/content/11/2/e038933.fullhttps://doaj.org/toc/2044-6055Introduction Intestinal microbiota is arising as a new element in the physiopathology of cardiovascular diseases. A healthy microbiota includes a balanced representation of bacteria with health promotion functions (symbiotes). The aim of this study is to analyse the relationship between intestinal microbiota composition and arterial stiffness.Methods and analysis An observational case—control study will be developed. Cases will be defined by the presence of at least one of the following: carotid-femoral pulse wave velocity (cf-PWV), Cardio-Ankle Vascular Index (CAVI), brachial ankle pulse wave velocity (ba or ba-PWV) above the 90th percentile, for age and sex, of the reference population. Controls will be selected from the same population as cases. The study will be developed in Primary Healthcare Centres. We will select 500 subjects (250 cases and 250 controls), between 45 and 74 years of age. Cases will be selected from a database that combines data from EVA study (Spain) and Guimarães/Vizela study (Portugal). Measurements: cf-PWV will be measured using the SphygmoCor system, CAVI, ba-PWV and Ankle-Brachial Index will be determined using VaSera device. Gut microbiome composition in faecal samples will be determined by 16S ribosomal RNA sequencing. Lifestyle will be assessed by food frequency questionnaire, adherence to the Mediterranean diet and IPAQ (International Physical Activity Questionnaire). Body composition will be evaluated by bioimpedance.Ethics and dissemination The study has been approved by ‘Committee of ethics of research with medicines of the health area of Salamanca’ on 14 December 2018 (cod. 2018-11-136) and the ’Ethics committee for health of Guimaraes’ (Portugal) on 15 October 2019 (ref: 67/2019). All study participants will sign an informed consent form agreeing to participate in the study, in compliance with the Declaration of Helsinki and the WHO standards for observational studies. The results of this study will allow a better description of gut microbiota in patients with arterial stiffness.Trial registration details ClinicalTrials.gov, identifier NCT03900338Luis Garcia-OrtizManuel A Gómez-MarcosEmiliano Rodriguez-SanchezRita SalvadoSandra Santos-MinguezCristina Agudo-CondeCristina Lugones-SanchezAngela Cabo-LasoJesus Mª Hernandez-SanchezRocio BenitoJesus M Hernandez-RivasPedro Guimarães CunhaMIVAS InvestigatorsBMJ Publishing GrouparticleMedicineRENBMJ Open, Vol 11, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Luis Garcia-Ortiz Manuel A Gómez-Marcos Emiliano Rodriguez-Sanchez Rita Salvado Sandra Santos-Minguez Cristina Agudo-Conde Cristina Lugones-Sanchez Angela Cabo-Laso Jesus Mª Hernandez-Sanchez Rocio Benito Jesus M Hernandez-Rivas Pedro Guimarães Cunha MIVAS Investigators Gut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (MIVAS study)
description	Introduction Intestinal microbiota is arising as a new element in the physiopathology of cardiovascular diseases. A healthy microbiota includes a balanced representation of bacteria with health promotion functions (symbiotes). The aim of this study is to analyse the relationship between intestinal microbiota composition and arterial stiffness.Methods and analysis An observational case—control study will be developed. Cases will be defined by the presence of at least one of the following: carotid-femoral pulse wave velocity (cf-PWV), Cardio-Ankle Vascular Index (CAVI), brachial ankle pulse wave velocity (ba or ba-PWV) above the 90th percentile, for age and sex, of the reference population. Controls will be selected from the same population as cases. The study will be developed in Primary Healthcare Centres. We will select 500 subjects (250 cases and 250 controls), between 45 and 74 years of age. Cases will be selected from a database that combines data from EVA study (Spain) and Guimarães/Vizela study (Portugal). Measurements: cf-PWV will be measured using the SphygmoCor system, CAVI, ba-PWV and Ankle-Brachial Index will be determined using VaSera device. Gut microbiome composition in faecal samples will be determined by 16S ribosomal RNA sequencing. Lifestyle will be assessed by food frequency questionnaire, adherence to the Mediterranean diet and IPAQ (International Physical Activity Questionnaire). Body composition will be evaluated by bioimpedance.Ethics and dissemination The study has been approved by ‘Committee of ethics of research with medicines of the health area of Salamanca’ on 14 December 2018 (cod. 2018-11-136) and the ’Ethics committee for health of Guimaraes’ (Portugal) on 15 October 2019 (ref: 67/2019). All study participants will sign an informed consent form agreeing to participate in the study, in compliance with the Declaration of Helsinki and the WHO standards for observational studies. The results of this study will allow a better description of gut microbiota in patients with arterial stiffness.Trial registration details ClinicalTrials.gov, identifier NCT03900338
format	article
author	Luis Garcia-Ortiz Manuel A Gómez-Marcos Emiliano Rodriguez-Sanchez Rita Salvado Sandra Santos-Minguez Cristina Agudo-Conde Cristina Lugones-Sanchez Angela Cabo-Laso Jesus Mª Hernandez-Sanchez Rocio Benito Jesus M Hernandez-Rivas Pedro Guimarães Cunha MIVAS Investigators
author_facet	Luis Garcia-Ortiz Manuel A Gómez-Marcos Emiliano Rodriguez-Sanchez Rita Salvado Sandra Santos-Minguez Cristina Agudo-Conde Cristina Lugones-Sanchez Angela Cabo-Laso Jesus Mª Hernandez-Sanchez Rocio Benito Jesus M Hernandez-Rivas Pedro Guimarães Cunha MIVAS Investigators
author_sort	Luis Garcia-Ortiz
title	Gut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (MIVAS study)
title_short	Gut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (MIVAS study)
title_full	Gut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (MIVAS study)
title_fullStr	Gut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (MIVAS study)
title_full_unstemmed	Gut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (MIVAS study)
title_sort	gut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (mivas study)
publisher	BMJ Publishing Group
publishDate	2021
url	https://doaj.org/article/32f768282dd348468e9dcc478c9db59e
work_keys_str_mv	AT luisgarciaortiz gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT manuelagomezmarcos gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT emilianorodriguezsanchez gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT ritasalvado gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT sandrasantosminguez gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT cristinaagudoconde gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT cristinalugonessanchez gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT angelacabolaso gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT jesusmahernandezsanchez gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT rociobenito gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT jesusmhernandezrivas gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT pedroguimaraescunha gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy AT mivasinvestigators gutmicrobiotacompositionandarterialstiffnessmeasuredbypulsewavevelocitycasecontrolstudyprotocolmivasstudy
_version_	1718420895345672192

Gut microbiota composition and arterial stiffness measured by pulse wave velocity: case–control study protocol (MIVAS study)

Ejemplares similares