Electro-Acupuncture on ST36 and SP6 Acupoints Ameliorates Lung Injury via Sciatic Nerve in a Rat Model of Limb Ischemia-Reperfusion

Wei Lin,1 Danyun Jia,2 Changchang Fu,1 Yihui Zheng,1 Zhenlang Lin1 1Department of Pediatrics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, People’s Republic of China; 2Department of Anesthesiology, The Fir...

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Autores principales:	Lin W, Jia D, Fu C, Zheng Y, Lin Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	electro-acupuncture limb ischemia-reperfusion injury sciatic nerve Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/32fcbbfb4e2749f18506170915892548
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spelling	oai:doaj.org-article:32fcbbfb4e2749f185061709158925482021-12-02T13:07:23ZElectro-Acupuncture on ST36 and SP6 Acupoints Ameliorates Lung Injury via Sciatic Nerve in a Rat Model of Limb Ischemia-Reperfusion1178-7031https://doaj.org/article/32fcbbfb4e2749f185061709158925482020-08-01T00:00:00Zhttps://www.dovepress.com/electro-acupuncture-on-st36-and-sp6-acupoints-ameliorates-lung-injury--peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Wei Lin,1 Danyun Jia,2 Changchang Fu,1 Yihui Zheng,1 Zhenlang Lin1 1Department of Pediatrics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, People’s Republic of China; 2Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, People’s Republic of ChinaCorrespondence: Zhenlang Lin Department of PediatricThe Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, People’s Republic of ChinaEmail lzlprof2020@163.comIntroduction: Electro-acupuncture (EA) can significantly improve inflammatory response, but the specific mechanism is not clear. Limb ischemia-reperfusion (I/R) first produces inflammatory reactions in the lungs. In this study, EA on Zusanli (ST36) and Sanyinjiao (SP6) were used to explore the mechanism of improving tissue inflammation by sciatic nerve disconnection.Material and Methods: A total of 56 male Sprague-Dawley rats were randomly divided into sham group, model group, EA group, SEA group, SNC+EA group, TNC+EA group and PNC+EA group. The sham groups were not given any treatment. Rats in the model group were treated with limb I/R without acupuncture intervention. In the EA group, ST36 and SP6 were given EA treatment for 30min before modeling. No electric current was given in the SEA group, and other operations were the same as those in the EA group. The SNC+EA group, TNC+EA group and PNC+EA group were respectively given sciatic nerve, tibial nerve or peroneal nerve amputation 72h before modeling, and the others were the same as the model group.Results: Compared with the sham group, PaO2 and a/A ratios decreased significantly in the model group (P < 0.05), while PA-aO2, RI, the ratio of wet to dry, lung injury value and inflammatory factor TNF-α, IL-1, IL-6, and MPO increased significantly (P < 0.05). Compared with the model group, PaO2, a/A ratios increased significantly in the EA group (P < 0.05), while PA-aO2, RI, the ratio of wet to dry lung, lung injury value, and TNF-α, IL-1, IL-6, and MPO decreased significantly (P < 0.05). After transection of the sciatic nerve, the protective effect of EA disappeared. However, when the peroneal or tibial nerve was severed, EA continued to maintain the protective effect.Conclusion: EA on ST36 and SP6 can alleviate lung injury caused by limb I/R through the sciatic nerve.Keywords: electro-acupuncture, limb ischemia-reperfusion injury, sciatic nerveLin WJia DFu CZheng YLin ZDove Medical Pressarticleelectro-acupuncturelimb ischemia-reperfusion injurysciatic nervePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 465-470 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	electro-acupuncture limb ischemia-reperfusion injury sciatic nerve Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	electro-acupuncture limb ischemia-reperfusion injury sciatic nerve Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Lin W Jia D Fu C Zheng Y Lin Z Electro-Acupuncture on ST36 and SP6 Acupoints Ameliorates Lung Injury via Sciatic Nerve in a Rat Model of Limb Ischemia-Reperfusion
description	Wei Lin,1 Danyun Jia,2 Changchang Fu,1 Yihui Zheng,1 Zhenlang Lin1 1Department of Pediatrics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, People’s Republic of China; 2Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, People’s Republic of ChinaCorrespondence: Zhenlang Lin Department of PediatricThe Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, People’s Republic of ChinaEmail lzlprof2020@163.comIntroduction: Electro-acupuncture (EA) can significantly improve inflammatory response, but the specific mechanism is not clear. Limb ischemia-reperfusion (I/R) first produces inflammatory reactions in the lungs. In this study, EA on Zusanli (ST36) and Sanyinjiao (SP6) were used to explore the mechanism of improving tissue inflammation by sciatic nerve disconnection.Material and Methods: A total of 56 male Sprague-Dawley rats were randomly divided into sham group, model group, EA group, SEA group, SNC+EA group, TNC+EA group and PNC+EA group. The sham groups were not given any treatment. Rats in the model group were treated with limb I/R without acupuncture intervention. In the EA group, ST36 and SP6 were given EA treatment for 30min before modeling. No electric current was given in the SEA group, and other operations were the same as those in the EA group. The SNC+EA group, TNC+EA group and PNC+EA group were respectively given sciatic nerve, tibial nerve or peroneal nerve amputation 72h before modeling, and the others were the same as the model group.Results: Compared with the sham group, PaO2 and a/A ratios decreased significantly in the model group (P < 0.05), while PA-aO2, RI, the ratio of wet to dry, lung injury value and inflammatory factor TNF-α, IL-1, IL-6, and MPO increased significantly (P < 0.05). Compared with the model group, PaO2, a/A ratios increased significantly in the EA group (P < 0.05), while PA-aO2, RI, the ratio of wet to dry lung, lung injury value, and TNF-α, IL-1, IL-6, and MPO decreased significantly (P < 0.05). After transection of the sciatic nerve, the protective effect of EA disappeared. However, when the peroneal or tibial nerve was severed, EA continued to maintain the protective effect.Conclusion: EA on ST36 and SP6 can alleviate lung injury caused by limb I/R through the sciatic nerve.Keywords: electro-acupuncture, limb ischemia-reperfusion injury, sciatic nerve
format	article
author	Lin W Jia D Fu C Zheng Y Lin Z
author_facet	Lin W Jia D Fu C Zheng Y Lin Z
author_sort	Lin W
title	Electro-Acupuncture on ST36 and SP6 Acupoints Ameliorates Lung Injury via Sciatic Nerve in a Rat Model of Limb Ischemia-Reperfusion
title_short	Electro-Acupuncture on ST36 and SP6 Acupoints Ameliorates Lung Injury via Sciatic Nerve in a Rat Model of Limb Ischemia-Reperfusion
title_full	Electro-Acupuncture on ST36 and SP6 Acupoints Ameliorates Lung Injury via Sciatic Nerve in a Rat Model of Limb Ischemia-Reperfusion
title_fullStr	Electro-Acupuncture on ST36 and SP6 Acupoints Ameliorates Lung Injury via Sciatic Nerve in a Rat Model of Limb Ischemia-Reperfusion
title_full_unstemmed	Electro-Acupuncture on ST36 and SP6 Acupoints Ameliorates Lung Injury via Sciatic Nerve in a Rat Model of Limb Ischemia-Reperfusion
title_sort	electro-acupuncture on st36 and sp6 acupoints ameliorates lung injury via sciatic nerve in a rat model of limb ischemia-reperfusion
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/32fcbbfb4e2749f18506170915892548
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Electro-Acupuncture on ST36 and SP6 Acupoints Ameliorates Lung Injury via Sciatic Nerve in a Rat Model of Limb Ischemia-Reperfusion

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