Altered Monocyte Subsets in Kawasaki Disease Revealed by Single-cell RNA-Sequencing
Zhimin Geng,* Yijing Tao,* Fenglei Zheng, Linlin Wu, Ying Wang, Yujia Wang, Yameng Sun, Songling Fu, Wei Wang, Chunhong Xie, Yiying Zhang, Fangqi Gong Department of Cardiology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health,...
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Dove Medical Press
2021
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oai:doaj.org-article:32ff80469df745e6bed7d53a2e262b852021-12-02T13:34:19ZAltered Monocyte Subsets in Kawasaki Disease Revealed by Single-cell RNA-Sequencing1178-7031https://doaj.org/article/32ff80469df745e6bed7d53a2e262b852021-03-01T00:00:00Zhttps://www.dovepress.com/altered-monocyte-subsets-in-kawasaki-disease-revealed-by-single-cellnb-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Zhimin Geng,* Yijing Tao,* Fenglei Zheng, Linlin Wu, Ying Wang, Yujia Wang, Yameng Sun, Songling Fu, Wei Wang, Chunhong Xie, Yiying Zhang, Fangqi Gong Department of Cardiology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, Zhejiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fangqi GongDepartment of Cardiology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, Zhejiang, People’s Republic of ChinaTel/Fax +86 571 86670008Email gongfangqi@zju.edu.cnBackground: Kawasaki disease (KD) is characterized by a disorder of immune response, and its etiology remains unknown. Monocyte is an important member of the body’s innate immune system; however its role in KD is still elusive due to its ambiguous heterogeneity and complex functions. We aim to comprehensively delineate monocyte heterogeneity in healthy and KD infants and to reveal the underlying mechanism for KD.Methods: Peripheral monocytes were enriched from peripheral blood samples of two healthy infants and two KD infants. scRNA-seq was performed to acquire the transcriptomic atlas of monocytes. Bio-information analysis was utilized to identify monocyte subsets and explore their functions and differentiation states. SELL+CD14+CD16- monocytes were validated using flow cytometry.Results: Three monocyte subsets were identified in healthy infants, including CD14+CD16- monocytes, CD14+CD16+ monocytes, and CD14LowCD16+ monocytes. Cell trajectory analysis revealed that the three monocyte subsets represent a linear differentiation, and possess different biological functions. Furthermore, SELL+CD14+CD16- monocytes, which were poorly differentiated and relating to neutrophil activation, were found to be expanded in KD.Conclusion: Our findings provide a valuable resource for deciphering the monocyte heterogeneity in healthy infants and uncover the altered monocyte subsets in KD patients, suggesting potential biomarkers for KD diagnosis and treatment.Keywords: Kawasaki disease, monocyte subsets, scRNA-seqGeng ZTao YZheng FWu LWang YWang YSun YFu SWang WXie CZhang YGong FDove Medical Pressarticlekawasaki diseasemonocyte subsetsscrna-seqPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 885-896 (2021) |
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kawasaki disease monocyte subsets scrna-seq Pathology RB1-214 Therapeutics. Pharmacology RM1-950 |
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kawasaki disease monocyte subsets scrna-seq Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Geng Z Tao Y Zheng F Wu L Wang Y Wang Y Sun Y Fu S Wang W Xie C Zhang Y Gong F Altered Monocyte Subsets in Kawasaki Disease Revealed by Single-cell RNA-Sequencing |
| description |
Zhimin Geng,* Yijing Tao,* Fenglei Zheng, Linlin Wu, Ying Wang, Yujia Wang, Yameng Sun, Songling Fu, Wei Wang, Chunhong Xie, Yiying Zhang, Fangqi Gong Department of Cardiology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, Zhejiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fangqi GongDepartment of Cardiology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, Zhejiang, People’s Republic of ChinaTel/Fax +86 571 86670008Email gongfangqi@zju.edu.cnBackground: Kawasaki disease (KD) is characterized by a disorder of immune response, and its etiology remains unknown. Monocyte is an important member of the body’s innate immune system; however its role in KD is still elusive due to its ambiguous heterogeneity and complex functions. We aim to comprehensively delineate monocyte heterogeneity in healthy and KD infants and to reveal the underlying mechanism for KD.Methods: Peripheral monocytes were enriched from peripheral blood samples of two healthy infants and two KD infants. scRNA-seq was performed to acquire the transcriptomic atlas of monocytes. Bio-information analysis was utilized to identify monocyte subsets and explore their functions and differentiation states. SELL+CD14+CD16- monocytes were validated using flow cytometry.Results: Three monocyte subsets were identified in healthy infants, including CD14+CD16- monocytes, CD14+CD16+ monocytes, and CD14LowCD16+ monocytes. Cell trajectory analysis revealed that the three monocyte subsets represent a linear differentiation, and possess different biological functions. Furthermore, SELL+CD14+CD16- monocytes, which were poorly differentiated and relating to neutrophil activation, were found to be expanded in KD.Conclusion: Our findings provide a valuable resource for deciphering the monocyte heterogeneity in healthy infants and uncover the altered monocyte subsets in KD patients, suggesting potential biomarkers for KD diagnosis and treatment.Keywords: Kawasaki disease, monocyte subsets, scRNA-seq |
| format |
article |
| author |
Geng Z Tao Y Zheng F Wu L Wang Y Wang Y Sun Y Fu S Wang W Xie C Zhang Y Gong F |
| author_facet |
Geng Z Tao Y Zheng F Wu L Wang Y Wang Y Sun Y Fu S Wang W Xie C Zhang Y Gong F |
| author_sort |
Geng Z |
| title |
Altered Monocyte Subsets in Kawasaki Disease Revealed by Single-cell RNA-Sequencing |
| title_short |
Altered Monocyte Subsets in Kawasaki Disease Revealed by Single-cell RNA-Sequencing |
| title_full |
Altered Monocyte Subsets in Kawasaki Disease Revealed by Single-cell RNA-Sequencing |
| title_fullStr |
Altered Monocyte Subsets in Kawasaki Disease Revealed by Single-cell RNA-Sequencing |
| title_full_unstemmed |
Altered Monocyte Subsets in Kawasaki Disease Revealed by Single-cell RNA-Sequencing |
| title_sort |
altered monocyte subsets in kawasaki disease revealed by single-cell rna-sequencing |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/32ff80469df745e6bed7d53a2e262b85 |
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