Diagnostic Potential and Interactive Dynamics of the Colorectal Cancer Virome

ABSTRACT Human viruses (those that infect human cells) have been associated with many cancers, largely due to their mutagenic and functionally manipulative abilities. Despite this, cancer microbiome studies have focused almost exclusively on bacteria instead of viruses. We began evaluating the cance...

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Autores principales:	Geoffrey D. Hannigan, Melissa B. Duhaime, Mack T. Ruffin, Charlie C. Koumpouras, Patrick D. Schloss
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	bacteriophage colorectal cancer diagnostic microbial ecology microbiome microbiota Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3301d56017244fe1906fd73064bf1f17
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spelling	oai:doaj.org-article:3301d56017244fe1906fd73064bf1f172021-11-15T15:52:19ZDiagnostic Potential and Interactive Dynamics of the Colorectal Cancer Virome10.1128/mBio.02248-182150-7511https://doaj.org/article/3301d56017244fe1906fd73064bf1f172018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02248-18https://doaj.org/toc/2150-7511ABSTRACT Human viruses (those that infect human cells) have been associated with many cancers, largely due to their mutagenic and functionally manipulative abilities. Despite this, cancer microbiome studies have focused almost exclusively on bacteria instead of viruses. We began evaluating the cancer virome by focusing on colorectal cancer, a primary cause of morbidity and mortality throughout the world and a cancer linked to altered colonic bacterial community compositions but with an unknown association with the gut virome. We used 16S rRNA gene, whole shotgun metagenomic, and purified virus metagenomic sequencing of stool to evaluate the differences in human colorectal cancer virus and bacterial community composition. Through random forest modeling, we identified differences in the healthy and colorectal cancer viromes. The cancer-associated virome consisted primarily of temperate bacteriophages that were also predicted to be bacterium-virus community network hubs. These results provide foundational evidence that bacteriophage communities are associated with colorectal cancer and potentially impact cancer progression by altering the bacterial host communities. IMPORTANCE Colorectal cancer is a leading cause of cancer-related death in the United States and worldwide. Its risk and severity have been linked to colonic bacterial community composition. Although human-specific viruses have been linked to other cancers and diseases, little is known about colorectal cancer virus communities. We addressed this knowledge gap by identifying differences in colonic virus communities in the stool of colorectal cancer patients and how they compared to bacterial community differences. The results suggested an indirect role for the virome in impacting colorectal cancer by modulating the associated bacterial community. These findings both support the idea of a biological role for viruses in colorectal cancer and provide a new understanding of basic colorectal cancer etiology.Geoffrey D. HanniganMelissa B. DuhaimeMack T. RuffinCharlie C. KoumpourasPatrick D. SchlossAmerican Society for Microbiologyarticlebacteriophagecolorectal cancerdiagnosticmicrobial ecologymicrobiomemicrobiotaMicrobiologyQR1-502ENmBio, Vol 9, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteriophage colorectal cancer diagnostic microbial ecology microbiome microbiota Microbiology QR1-502
spellingShingle	bacteriophage colorectal cancer diagnostic microbial ecology microbiome microbiota Microbiology QR1-502 Geoffrey D. Hannigan Melissa B. Duhaime Mack T. Ruffin Charlie C. Koumpouras Patrick D. Schloss Diagnostic Potential and Interactive Dynamics of the Colorectal Cancer Virome
description	ABSTRACT Human viruses (those that infect human cells) have been associated with many cancers, largely due to their mutagenic and functionally manipulative abilities. Despite this, cancer microbiome studies have focused almost exclusively on bacteria instead of viruses. We began evaluating the cancer virome by focusing on colorectal cancer, a primary cause of morbidity and mortality throughout the world and a cancer linked to altered colonic bacterial community compositions but with an unknown association with the gut virome. We used 16S rRNA gene, whole shotgun metagenomic, and purified virus metagenomic sequencing of stool to evaluate the differences in human colorectal cancer virus and bacterial community composition. Through random forest modeling, we identified differences in the healthy and colorectal cancer viromes. The cancer-associated virome consisted primarily of temperate bacteriophages that were also predicted to be bacterium-virus community network hubs. These results provide foundational evidence that bacteriophage communities are associated with colorectal cancer and potentially impact cancer progression by altering the bacterial host communities. IMPORTANCE Colorectal cancer is a leading cause of cancer-related death in the United States and worldwide. Its risk and severity have been linked to colonic bacterial community composition. Although human-specific viruses have been linked to other cancers and diseases, little is known about colorectal cancer virus communities. We addressed this knowledge gap by identifying differences in colonic virus communities in the stool of colorectal cancer patients and how they compared to bacterial community differences. The results suggested an indirect role for the virome in impacting colorectal cancer by modulating the associated bacterial community. These findings both support the idea of a biological role for viruses in colorectal cancer and provide a new understanding of basic colorectal cancer etiology.
format	article
author	Geoffrey D. Hannigan Melissa B. Duhaime Mack T. Ruffin Charlie C. Koumpouras Patrick D. Schloss
author_facet	Geoffrey D. Hannigan Melissa B. Duhaime Mack T. Ruffin Charlie C. Koumpouras Patrick D. Schloss
author_sort	Geoffrey D. Hannigan
title	Diagnostic Potential and Interactive Dynamics of the Colorectal Cancer Virome
title_short	Diagnostic Potential and Interactive Dynamics of the Colorectal Cancer Virome
title_full	Diagnostic Potential and Interactive Dynamics of the Colorectal Cancer Virome
title_fullStr	Diagnostic Potential and Interactive Dynamics of the Colorectal Cancer Virome
title_full_unstemmed	Diagnostic Potential and Interactive Dynamics of the Colorectal Cancer Virome
title_sort	diagnostic potential and interactive dynamics of the colorectal cancer virome
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/3301d56017244fe1906fd73064bf1f17
work_keys_str_mv	AT geoffreydhannigan diagnosticpotentialandinteractivedynamicsofthecolorectalcancervirome AT melissabduhaime diagnosticpotentialandinteractivedynamicsofthecolorectalcancervirome AT macktruffin diagnosticpotentialandinteractivedynamicsofthecolorectalcancervirome AT charlieckoumpouras diagnosticpotentialandinteractivedynamicsofthecolorectalcancervirome AT patrickdschloss diagnosticpotentialandinteractivedynamicsofthecolorectalcancervirome
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Diagnostic Potential and Interactive Dynamics of the Colorectal Cancer Virome

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