New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.

<h4>Background</h4>The aim of this article is to present an optimized acquisition and analysis protocol for the echocardiographic evaluation of left ventricle (LV) remodeling in a mouse model of myocardial infarction (MI).<h4>Methodology</h4>13 female DBA/2J mice underwent pe...

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Autores principales:	Carolina Benavides-Vallve, David Corbacho, Olalla Iglesias-Garcia, Beatriz Pelacho, Edurne Albiasu, Sara Castaño, Arrate Muñoz-Barrutia, Felipe Prosper, Carlos Ortiz-de-Solorzano
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33065706a861426b96655fdcf323e6a9
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spelling	oai:doaj.org-article:33065706a861426b96655fdcf323e6a92021-11-18T07:10:42ZNew strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.1932-620310.1371/journal.pone.0041691https://doaj.org/article/33065706a861426b96655fdcf323e6a92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22848568/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The aim of this article is to present an optimized acquisition and analysis protocol for the echocardiographic evaluation of left ventricle (LV) remodeling in a mouse model of myocardial infarction (MI).<h4>Methodology</h4>13 female DBA/2J mice underwent permanent occlusion of the left anterior descending (LAD) coronary artery leading to MI. Mice echocardiography was performed using a Vevo 770 (Visualsonics, Canada) before infarction, and 7, 14, 30, 60, 90 and 120 days after LAD ligation. LV systolic function was evaluated using different parameters, including the fractional area change (FAC%) computed in four high-temporal resolution B-mode short axis images taken at different ventricular levels, and in one parasternal long axis. Pulsed wave and tissue Doppler modes were used to evaluate the diastolic function and Tei Index for global cardiac function. The echocardiographic measurements of infarct size were validated histologically using collagen deposition labeled by Sirius red staining. All data was analyzed using Shapiro-Wilk and Student's t-tests.<h4>Principal findings</h4>Our results reveal LV dilation resulting in marked remodeling an severe systolic dysfunction, starting seven days after MI (LV internal apical diameter, basal = 2.82±0.24, 7d = 3.49±0.42; p<0.001. End-diastolic area, basal = 18.98±1.81, 7d = 22.04±2.11; p<0.001). A strong statistically significant negative correlation exists between the infarct size and long-axis FAC% (r = -0.946; R(2) = 0.90; p<0.05). Moreover, the measured Tei Index values confirmed significant post-infarction impairment of the global cardiac function (basal = 0.46±0.07, 7d = 0.55±0.08, 14 d = 0.57±0.06, 30 d = 0.54±0.06, 60 d = 0.54±0.07, 90 d = 0.57±0.08; p<0.01).<h4>Conclusions/significance</h4>In summary, we have performed a complete characterization of LV post-infarction remodeling in a DBA/2J mouse model of MI, using parameters adapted to the particular characteristics of the model In the future, this well characterized model will be used in both investigative and pharmacological studies that require accurate quantitative monitoring of cardiac recovery after myocardial infarction.Carolina Benavides-VallveDavid CorbachoOlalla Iglesias-GarciaBeatriz PelachoEdurne AlbiasuSara CastañoArrate Muñoz-BarrutiaFelipe ProsperCarlos Ortiz-de-SolorzanoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41691 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carolina Benavides-Vallve David Corbacho Olalla Iglesias-Garcia Beatriz Pelacho Edurne Albiasu Sara Castaño Arrate Muñoz-Barrutia Felipe Prosper Carlos Ortiz-de-Solorzano New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.
description	<h4>Background</h4>The aim of this article is to present an optimized acquisition and analysis protocol for the echocardiographic evaluation of left ventricle (LV) remodeling in a mouse model of myocardial infarction (MI).<h4>Methodology</h4>13 female DBA/2J mice underwent permanent occlusion of the left anterior descending (LAD) coronary artery leading to MI. Mice echocardiography was performed using a Vevo 770 (Visualsonics, Canada) before infarction, and 7, 14, 30, 60, 90 and 120 days after LAD ligation. LV systolic function was evaluated using different parameters, including the fractional area change (FAC%) computed in four high-temporal resolution B-mode short axis images taken at different ventricular levels, and in one parasternal long axis. Pulsed wave and tissue Doppler modes were used to evaluate the diastolic function and Tei Index for global cardiac function. The echocardiographic measurements of infarct size were validated histologically using collagen deposition labeled by Sirius red staining. All data was analyzed using Shapiro-Wilk and Student's t-tests.<h4>Principal findings</h4>Our results reveal LV dilation resulting in marked remodeling an severe systolic dysfunction, starting seven days after MI (LV internal apical diameter, basal = 2.82±0.24, 7d = 3.49±0.42; p<0.001. End-diastolic area, basal = 18.98±1.81, 7d = 22.04±2.11; p<0.001). A strong statistically significant negative correlation exists between the infarct size and long-axis FAC% (r = -0.946; R(2) = 0.90; p<0.05). Moreover, the measured Tei Index values confirmed significant post-infarction impairment of the global cardiac function (basal = 0.46±0.07, 7d = 0.55±0.08, 14 d = 0.57±0.06, 30 d = 0.54±0.06, 60 d = 0.54±0.07, 90 d = 0.57±0.08; p<0.01).<h4>Conclusions/significance</h4>In summary, we have performed a complete characterization of LV post-infarction remodeling in a DBA/2J mouse model of MI, using parameters adapted to the particular characteristics of the model In the future, this well characterized model will be used in both investigative and pharmacological studies that require accurate quantitative monitoring of cardiac recovery after myocardial infarction.
format	article
author	Carolina Benavides-Vallve David Corbacho Olalla Iglesias-Garcia Beatriz Pelacho Edurne Albiasu Sara Castaño Arrate Muñoz-Barrutia Felipe Prosper Carlos Ortiz-de-Solorzano
author_facet	Carolina Benavides-Vallve David Corbacho Olalla Iglesias-Garcia Beatriz Pelacho Edurne Albiasu Sara Castaño Arrate Muñoz-Barrutia Felipe Prosper Carlos Ortiz-de-Solorzano
author_sort	Carolina Benavides-Vallve
title	New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.
title_short	New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.
title_full	New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.
title_fullStr	New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.
title_full_unstemmed	New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.
title_sort	new strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/33065706a861426b96655fdcf323e6a9
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New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.

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