Development of a novel heterologous gene expression system using earthworms

Abstract In recent years, animals and plants have received increasing attention as potential next-generation protein production systems, especially for biopharmaceuticals and animal proteins. The aim of the present study was to develop the earthworms Eisenia fetida Waki and Eisenia andrei Sagami as...

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Autores principales:	Shin-ichi Akazawa, Yu Machida, Aya Takeuchi, Yuka Wakatsuki, Naoki Kanda, Norito Kashima, Hayato Murayama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33157ed90b2a4aef93282839da6e6760
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spelling	oai:doaj.org-article:33157ed90b2a4aef93282839da6e67602021-12-02T15:51:12ZDevelopment of a novel heterologous gene expression system using earthworms10.1038/s41598-021-87641-w2045-2322https://doaj.org/article/33157ed90b2a4aef93282839da6e67602021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87641-whttps://doaj.org/toc/2045-2322Abstract In recent years, animals and plants have received increasing attention as potential next-generation protein production systems, especially for biopharmaceuticals and animal proteins. The aim of the present study was to develop the earthworms Eisenia fetida Waki and Eisenia andrei Sagami as next-generation animal protein production hosts. These earthworms have been approved as model animals for acute toxicity tests by the Organization for Economic Co-operation and Development, and they have post-translational modification systems. However, so far, none of the studies have used earthworm transfection techniques. Thus, we developed a transfection method for E. fetida and E. andrei using microinjection and electroporation systems. The maximum survival rates and transfection efficiencies were 79.2% and 29.2% for E. fetida, and 95.8% and 50.0% for E. andrei, respectively. Furthermore, human erythropoietin was detected in the transformed earthworm tail fragments using an enzyme-linked immunosorbent assay. These results contribute to the development of a potential earthworm-based novel animal protein production system.Shin-ichi AkazawaYu MachidaAya TakeuchiYuka WakatsukiNaoki KandaNorito KashimaHayato MurayamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shin-ichi Akazawa Yu Machida Aya Takeuchi Yuka Wakatsuki Naoki Kanda Norito Kashima Hayato Murayama Development of a novel heterologous gene expression system using earthworms
description	Abstract In recent years, animals and plants have received increasing attention as potential next-generation protein production systems, especially for biopharmaceuticals and animal proteins. The aim of the present study was to develop the earthworms Eisenia fetida Waki and Eisenia andrei Sagami as next-generation animal protein production hosts. These earthworms have been approved as model animals for acute toxicity tests by the Organization for Economic Co-operation and Development, and they have post-translational modification systems. However, so far, none of the studies have used earthworm transfection techniques. Thus, we developed a transfection method for E. fetida and E. andrei using microinjection and electroporation systems. The maximum survival rates and transfection efficiencies were 79.2% and 29.2% for E. fetida, and 95.8% and 50.0% for E. andrei, respectively. Furthermore, human erythropoietin was detected in the transformed earthworm tail fragments using an enzyme-linked immunosorbent assay. These results contribute to the development of a potential earthworm-based novel animal protein production system.
format	article
author	Shin-ichi Akazawa Yu Machida Aya Takeuchi Yuka Wakatsuki Naoki Kanda Norito Kashima Hayato Murayama
author_facet	Shin-ichi Akazawa Yu Machida Aya Takeuchi Yuka Wakatsuki Naoki Kanda Norito Kashima Hayato Murayama
author_sort	Shin-ichi Akazawa
title	Development of a novel heterologous gene expression system using earthworms
title_short	Development of a novel heterologous gene expression system using earthworms
title_full	Development of a novel heterologous gene expression system using earthworms
title_fullStr	Development of a novel heterologous gene expression system using earthworms
title_full_unstemmed	Development of a novel heterologous gene expression system using earthworms
title_sort	development of a novel heterologous gene expression system using earthworms
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/33157ed90b2a4aef93282839da6e6760
work_keys_str_mv	AT shinichiakazawa developmentofanovelheterologousgeneexpressionsystemusingearthworms AT yumachida developmentofanovelheterologousgeneexpressionsystemusingearthworms AT ayatakeuchi developmentofanovelheterologousgeneexpressionsystemusingearthworms AT yukawakatsuki developmentofanovelheterologousgeneexpressionsystemusingearthworms AT naokikanda developmentofanovelheterologousgeneexpressionsystemusingearthworms AT noritokashima developmentofanovelheterologousgeneexpressionsystemusingearthworms AT hayatomurayama developmentofanovelheterologousgeneexpressionsystemusingearthworms
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Development of a novel heterologous gene expression system using earthworms

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