60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube

Direct detection of long-strand DNA by surface-enhanced Raman scattering (SERS) is a valuable method for diagnosis of hereditary diseases, but it is currently limited to less than 25-nt DNA strand in pure water, which makes this approach unsuitable for many real-life applications. Here, we report a...

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Autores principales:	Jikai Mao, Lvtao Huang, Li Fan, Fang Chen, Jingan Lou, Xuliang Shan, Dongdong Yu, Jianguang Zhou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	60-nt DNA direct detection surface-enhance Raman scattering Ag microcrystal polycationic Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/3318173b322641d6b71459c2e9835cd2
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id	oai:doaj.org-article:3318173b322641d6b71459c2e9835cd2
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spelling	oai:doaj.org-article:3318173b322641d6b71459c2e9835cd22021-11-25T18:27:16Z60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube10.3390/molecules262267901420-3049https://doaj.org/article/3318173b322641d6b71459c2e9835cd22021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6790https://doaj.org/toc/1420-3049Direct detection of long-strand DNA by surface-enhanced Raman scattering (SERS) is a valuable method for diagnosis of hereditary diseases, but it is currently limited to less than 25-nt DNA strand in pure water, which makes this approach unsuitable for many real-life applications. Here, we report a 60-nt DNA label-free detection strategy without pretreatment by SERS with polyquaternium-modified Ag microcrystals derived from an AgCl cube. Through the reduction-induced decomposition, the size of the about 3 × 3 × 3 μm<sup>3</sup> AgCl cube is reduced to Ag, and the surface is distributed with the uniform size of 63 nm silver nanoparticles, providing a large area of a robust and highly electromagnetic enhancement region. The modified polycationic molecule enhances the non-specific electrostatic interaction with the phosphate group, thereby anchoring DNA strands firmly to the SERS enhanced region intactly. As a result, the single-base recognition ability of this strategy reaches 60-nt and is successfully applied to detect thalassemia-related mutation genes.Jikai MaoLvtao HuangLi FanFang ChenJingan LouXuliang ShanDongdong YuJianguang ZhouMDPI AGarticle60-nt DNA direct detectionsurface-enhance Raman scatteringAg microcrystalpolycationicOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6790, p 6790 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	60-nt DNA direct detection surface-enhance Raman scattering Ag microcrystal polycationic Organic chemistry QD241-441
spellingShingle	60-nt DNA direct detection surface-enhance Raman scattering Ag microcrystal polycationic Organic chemistry QD241-441 Jikai Mao Lvtao Huang Li Fan Fang Chen Jingan Lou Xuliang Shan Dongdong Yu Jianguang Zhou 60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube
description	Direct detection of long-strand DNA by surface-enhanced Raman scattering (SERS) is a valuable method for diagnosis of hereditary diseases, but it is currently limited to less than 25-nt DNA strand in pure water, which makes this approach unsuitable for many real-life applications. Here, we report a 60-nt DNA label-free detection strategy without pretreatment by SERS with polyquaternium-modified Ag microcrystals derived from an AgCl cube. Through the reduction-induced decomposition, the size of the about 3 × 3 × 3 μm<sup>3</sup> AgCl cube is reduced to Ag, and the surface is distributed with the uniform size of 63 nm silver nanoparticles, providing a large area of a robust and highly electromagnetic enhancement region. The modified polycationic molecule enhances the non-specific electrostatic interaction with the phosphate group, thereby anchoring DNA strands firmly to the SERS enhanced region intactly. As a result, the single-base recognition ability of this strategy reaches 60-nt and is successfully applied to detect thalassemia-related mutation genes.
format	article
author	Jikai Mao Lvtao Huang Li Fan Fang Chen Jingan Lou Xuliang Shan Dongdong Yu Jianguang Zhou
author_facet	Jikai Mao Lvtao Huang Li Fan Fang Chen Jingan Lou Xuliang Shan Dongdong Yu Jianguang Zhou
author_sort	Jikai Mao
title	60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube
title_short	60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube
title_full	60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube
title_fullStr	60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube
title_full_unstemmed	60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube
title_sort	60-nt dna direct detection without pretreatment by surface-enhanced raman scattering with polycationic modified ag microcrystal derived from agcl cube
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3318173b322641d6b71459c2e9835cd2
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_version_	1718411126468771840

60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube

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