Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model

The metaproteome profiling of cecal contents collected from neonatal piglets fed pasteurized human milk (HM) or a dairy-based infant formula (MF) from postnatal day (PND) 2 to 21 were assessed. At PND 21, a subset of piglets from each group (<i>n</i> = 11/group) were euthanized, and ceca...

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Autores principales:	Fernanda Rosa, Boris L. Zybailov, Galina V. Glazko, Yasir Rahmatallah, Stephanie Byrum, Samuel G. Mackintosh, Anne K. Bowlin, Laxmi Yeruva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	neonates intestinal microbiota metaproteome Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/33198d0bc4304d039328639735d7270b
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spelling	oai:doaj.org-article:33198d0bc4304d039328639735d7270b2021-11-25T18:33:25ZMilk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model10.3390/nu131137182072-6643https://doaj.org/article/33198d0bc4304d039328639735d7270b2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3718https://doaj.org/toc/2072-6643The metaproteome profiling of cecal contents collected from neonatal piglets fed pasteurized human milk (HM) or a dairy-based infant formula (MF) from postnatal day (PND) 2 to 21 were assessed. At PND 21, a subset of piglets from each group (<i>n</i> = 11/group) were euthanized, and cecal contents were collected for further metaproteome analysis. Cecal microbiota composition showed predominantly more Firmicutes phyla and <i>Lachnospiraceae</i> family in the lumen of cecum of HM-fed piglets in comparison to the MF-fed group. <i>Ruminococcus</i> <i>gnavus</i> was the most abundant species from the Firmicutes phyla in the cecal contents of the HM-fed piglets at 21 days of age. A greater number of expressed proteins were identified in the cecal contents of the HM-fed piglets relative to the MF-fed piglets. Greater abundances of proteins potentially expressed by <i>Bacteroides</i> spp. such as glycoside enzymes were noted in the cecal lumen of HM-fed piglets relative to the MF. Additionally, lyases associated with <i>Lachnospiraceae</i> family were abundant in the cecum of the HM group relative to the MF group. Overall, our findings indicate that neonatal diet impacts the gut bacterial taxa and microbial proteins prior to weaning. The metaproteomics data were deposited into PRIDE, PXD025432 and 10.6019/PXD025432.Fernanda RosaBoris L. ZybailovGalina V. GlazkoYasir RahmatallahStephanie ByrumSamuel G. MackintoshAnne K. BowlinLaxmi YeruvaMDPI AGarticleneonatesintestinalmicrobiotametaproteomeNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3718, p 3718 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neonates intestinal microbiota metaproteome Nutrition. Foods and food supply TX341-641
spellingShingle	neonates intestinal microbiota metaproteome Nutrition. Foods and food supply TX341-641 Fernanda Rosa Boris L. Zybailov Galina V. Glazko Yasir Rahmatallah Stephanie Byrum Samuel G. Mackintosh Anne K. Bowlin Laxmi Yeruva Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model
description	The metaproteome profiling of cecal contents collected from neonatal piglets fed pasteurized human milk (HM) or a dairy-based infant formula (MF) from postnatal day (PND) 2 to 21 were assessed. At PND 21, a subset of piglets from each group (<i>n</i> = 11/group) were euthanized, and cecal contents were collected for further metaproteome analysis. Cecal microbiota composition showed predominantly more Firmicutes phyla and <i>Lachnospiraceae</i> family in the lumen of cecum of HM-fed piglets in comparison to the MF-fed group. <i>Ruminococcus</i> <i>gnavus</i> was the most abundant species from the Firmicutes phyla in the cecal contents of the HM-fed piglets at 21 days of age. A greater number of expressed proteins were identified in the cecal contents of the HM-fed piglets relative to the MF-fed piglets. Greater abundances of proteins potentially expressed by <i>Bacteroides</i> spp. such as glycoside enzymes were noted in the cecal lumen of HM-fed piglets relative to the MF. Additionally, lyases associated with <i>Lachnospiraceae</i> family were abundant in the cecum of the HM group relative to the MF group. Overall, our findings indicate that neonatal diet impacts the gut bacterial taxa and microbial proteins prior to weaning. The metaproteomics data were deposited into PRIDE, PXD025432 and 10.6019/PXD025432.
format	article
author	Fernanda Rosa Boris L. Zybailov Galina V. Glazko Yasir Rahmatallah Stephanie Byrum Samuel G. Mackintosh Anne K. Bowlin Laxmi Yeruva
author_facet	Fernanda Rosa Boris L. Zybailov Galina V. Glazko Yasir Rahmatallah Stephanie Byrum Samuel G. Mackintosh Anne K. Bowlin Laxmi Yeruva
author_sort	Fernanda Rosa
title	Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model
title_short	Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model
title_full	Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model
title_fullStr	Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model
title_full_unstemmed	Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model
title_sort	milk formula diet alters bacterial and host protein profile in comparison to human milk diet in neonatal piglet model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/33198d0bc4304d039328639735d7270b
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Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model

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