Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation.
Nematode-trapping fungi are "carnivorous" and attack their hosts using specialized trapping devices. The morphological development of these traps is the key indicator of their switch from saprophytic to predacious lifestyles. Here, the genome of the nematode-trapping fungus Arthrobotrys ol...
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2011
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oai:doaj.org-article:331a6cfeb68443c09b72f33b77a1ba5e2021-11-18T06:03:04ZGenomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation.1553-73661553-737410.1371/journal.ppat.1002179https://doaj.org/article/331a6cfeb68443c09b72f33b77a1ba5e2011-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21909256/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Nematode-trapping fungi are "carnivorous" and attack their hosts using specialized trapping devices. The morphological development of these traps is the key indicator of their switch from saprophytic to predacious lifestyles. Here, the genome of the nematode-trapping fungus Arthrobotrys oligospora Fres. (ATCC24927) was reported. The genome contains 40.07 Mb assembled sequence with 11,479 predicted genes. Comparative analysis showed that A. oligospora shared many more genes with pathogenic fungi than with non-pathogenic fungi. Specifically, compared to several sequenced ascomycete fungi, the A. oligospora genome has a larger number of pathogenicity-related genes in the subtilisin, cellulase, cellobiohydrolase, and pectinesterase gene families. Searching against the pathogen-host interaction gene database identified 398 homologous genes involved in pathogenicity in other fungi. The analysis of repetitive sequences provided evidence for repeat-induced point mutations in A. oligospora. Proteomic and quantitative PCR (qPCR) analyses revealed that 90 genes were significantly up-regulated at the early stage of trap-formation by nematode extracts and most of these genes were involved in translation, amino acid metabolism, carbohydrate metabolism, cell wall and membrane biogenesis. Based on the combined genomic, proteomic and qPCR data, a model for the formation of nematode trapping device in this fungus was proposed. In this model, multiple fungal signal transduction pathways are activated by its nematode prey to further regulate downstream genes associated with diverse cellular processes such as energy metabolism, biosynthesis of the cell wall and adhesive proteins, cell division, glycerol accumulation and peroxisome biogenesis. This study will facilitate the identification of pathogenicity-related genes and provide a broad foundation for understanding the molecular and evolutionary mechanisms underlying fungi-nematodes interactions.Jinkui YangLei WangXinglai JiYun FengXiaomin LiChenggang ZouJianping XuYan RenQili MiJunli WuShuqun LiuYu LiuXiaowei HuangHaiyan WangXuemei NiuJuan LiLianming LiangYanlu LuoKaifang JiWei ZhouZefen YuGuohong LiYajun LiuLei LiMin QiaoLu FengKe-Qin ZhangPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 9, p e1002179 (2011) |
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Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
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Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Jinkui Yang Lei Wang Xinglai Ji Yun Feng Xiaomin Li Chenggang Zou Jianping Xu Yan Ren Qili Mi Junli Wu Shuqun Liu Yu Liu Xiaowei Huang Haiyan Wang Xuemei Niu Juan Li Lianming Liang Yanlu Luo Kaifang Ji Wei Zhou Zefen Yu Guohong Li Yajun Liu Lei Li Min Qiao Lu Feng Ke-Qin Zhang Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. |
| description |
Nematode-trapping fungi are "carnivorous" and attack their hosts using specialized trapping devices. The morphological development of these traps is the key indicator of their switch from saprophytic to predacious lifestyles. Here, the genome of the nematode-trapping fungus Arthrobotrys oligospora Fres. (ATCC24927) was reported. The genome contains 40.07 Mb assembled sequence with 11,479 predicted genes. Comparative analysis showed that A. oligospora shared many more genes with pathogenic fungi than with non-pathogenic fungi. Specifically, compared to several sequenced ascomycete fungi, the A. oligospora genome has a larger number of pathogenicity-related genes in the subtilisin, cellulase, cellobiohydrolase, and pectinesterase gene families. Searching against the pathogen-host interaction gene database identified 398 homologous genes involved in pathogenicity in other fungi. The analysis of repetitive sequences provided evidence for repeat-induced point mutations in A. oligospora. Proteomic and quantitative PCR (qPCR) analyses revealed that 90 genes were significantly up-regulated at the early stage of trap-formation by nematode extracts and most of these genes were involved in translation, amino acid metabolism, carbohydrate metabolism, cell wall and membrane biogenesis. Based on the combined genomic, proteomic and qPCR data, a model for the formation of nematode trapping device in this fungus was proposed. In this model, multiple fungal signal transduction pathways are activated by its nematode prey to further regulate downstream genes associated with diverse cellular processes such as energy metabolism, biosynthesis of the cell wall and adhesive proteins, cell division, glycerol accumulation and peroxisome biogenesis. This study will facilitate the identification of pathogenicity-related genes and provide a broad foundation for understanding the molecular and evolutionary mechanisms underlying fungi-nematodes interactions. |
| format |
article |
| author |
Jinkui Yang Lei Wang Xinglai Ji Yun Feng Xiaomin Li Chenggang Zou Jianping Xu Yan Ren Qili Mi Junli Wu Shuqun Liu Yu Liu Xiaowei Huang Haiyan Wang Xuemei Niu Juan Li Lianming Liang Yanlu Luo Kaifang Ji Wei Zhou Zefen Yu Guohong Li Yajun Liu Lei Li Min Qiao Lu Feng Ke-Qin Zhang |
| author_facet |
Jinkui Yang Lei Wang Xinglai Ji Yun Feng Xiaomin Li Chenggang Zou Jianping Xu Yan Ren Qili Mi Junli Wu Shuqun Liu Yu Liu Xiaowei Huang Haiyan Wang Xuemei Niu Juan Li Lianming Liang Yanlu Luo Kaifang Ji Wei Zhou Zefen Yu Guohong Li Yajun Liu Lei Li Min Qiao Lu Feng Ke-Qin Zhang |
| author_sort |
Jinkui Yang |
| title |
Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. |
| title_short |
Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. |
| title_full |
Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. |
| title_fullStr |
Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. |
| title_full_unstemmed |
Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. |
| title_sort |
genomic and proteomic analyses of the fungus arthrobotrys oligospora provide insights into nematode-trap formation. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/331a6cfeb68443c09b72f33b77a1ba5e |
| work_keys_str_mv |
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