Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel

Abstract Spinal cord regeneration is limited due to various obstacles and complex pathophysiological events after injury. Combination therapy is one approach that recently garnered attention for spinal cord injury (SCI) recovery. A composite of three-dimensional (3D) collagen hydrogel containing epo...

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Autores principales:	Narges Mahmoodi, Jafar Ai, Zahra Hassannejad, Somayeh Ebrahimi-Barough, Elham Hasanzadeh, Houra Nekounam, Alexander R. Vaccaro, Vafa Rahimi-Movaghar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3327e6be3a754095b2ddf46bee0b4ac8
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spelling	oai:doaj.org-article:3327e6be3a754095b2ddf46bee0b4ac82021-11-08T10:54:49ZImproving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel10.1038/s41598-021-01071-22045-2322https://doaj.org/article/3327e6be3a754095b2ddf46bee0b4ac82021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01071-2https://doaj.org/toc/2045-2322Abstract Spinal cord regeneration is limited due to various obstacles and complex pathophysiological events after injury. Combination therapy is one approach that recently garnered attention for spinal cord injury (SCI) recovery. A composite of three-dimensional (3D) collagen hydrogel containing epothilone B (EpoB)-loaded polycaprolactone (PCL) microspheres (2.5 ng/mg, 10 ng/mg, and 40 ng/mg EpoB/PCL) were fabricated and optimized to improve motor neuron (MN) differentiation efficacy of human endometrial stem cells (hEnSCs). The microspheres were characterized using liquid chromatography-mass/mass spectrometry (LC-mas/mas) to assess the drug release and scanning electron microscope (SEM) for morphological assessment. hEnSCs were isolated, then characterized by flow cytometry, and seeded on the optimized 3D composite. Based on cell morphology and proliferation, cross-linked collagen hydrogels with and without 2.5 ng/mg EpoB loaded PCL microspheres were selected as the optimized formulations to compare the effect of EpoB release on MN differentiation. After differentiation, the expression of MN markers was estimated by real-time PCR and immunofluorescence (IF). The collagen hydrogel containing the EpoB group had the highest HB9 and ISL-1 expression and the longest neurite elongation. Providing a 3D permissive environment with EpoB, significantly improves MN-like cell differentiation and maturation of hEnSCs and is a promising approach to replace lost neurons after SCI.Narges MahmoodiJafar AiZahra HassannejadSomayeh Ebrahimi-BaroughElham HasanzadehHoura NekounamAlexander R. VaccaroVafa Rahimi-MovagharNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-21 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Narges Mahmoodi Jafar Ai Zahra Hassannejad Somayeh Ebrahimi-Barough Elham Hasanzadeh Houra Nekounam Alexander R. Vaccaro Vafa Rahimi-Movaghar Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel
description	Abstract Spinal cord regeneration is limited due to various obstacles and complex pathophysiological events after injury. Combination therapy is one approach that recently garnered attention for spinal cord injury (SCI) recovery. A composite of three-dimensional (3D) collagen hydrogel containing epothilone B (EpoB)-loaded polycaprolactone (PCL) microspheres (2.5 ng/mg, 10 ng/mg, and 40 ng/mg EpoB/PCL) were fabricated and optimized to improve motor neuron (MN) differentiation efficacy of human endometrial stem cells (hEnSCs). The microspheres were characterized using liquid chromatography-mass/mass spectrometry (LC-mas/mas) to assess the drug release and scanning electron microscope (SEM) for morphological assessment. hEnSCs were isolated, then characterized by flow cytometry, and seeded on the optimized 3D composite. Based on cell morphology and proliferation, cross-linked collagen hydrogels with and without 2.5 ng/mg EpoB loaded PCL microspheres were selected as the optimized formulations to compare the effect of EpoB release on MN differentiation. After differentiation, the expression of MN markers was estimated by real-time PCR and immunofluorescence (IF). The collagen hydrogel containing the EpoB group had the highest HB9 and ISL-1 expression and the longest neurite elongation. Providing a 3D permissive environment with EpoB, significantly improves MN-like cell differentiation and maturation of hEnSCs and is a promising approach to replace lost neurons after SCI.
format	article
author	Narges Mahmoodi Jafar Ai Zahra Hassannejad Somayeh Ebrahimi-Barough Elham Hasanzadeh Houra Nekounam Alexander R. Vaccaro Vafa Rahimi-Movaghar
author_facet	Narges Mahmoodi Jafar Ai Zahra Hassannejad Somayeh Ebrahimi-Barough Elham Hasanzadeh Houra Nekounam Alexander R. Vaccaro Vafa Rahimi-Movaghar
author_sort	Narges Mahmoodi
title	Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel
title_short	Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel
title_full	Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel
title_fullStr	Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel
title_full_unstemmed	Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel
title_sort	improving motor neuron-like cell differentiation of henscs by the combination of epothilone b loaded pcl microspheres in optimized 3d collagen hydrogel
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3327e6be3a754095b2ddf46bee0b4ac8
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Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel

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