Small RNA sequencing evaluation of renal microRNA biomarkers in dogs with X-linked hereditary nephropathy

Abstract Dogs with X-linked hereditary nephropathy (XLHN) are an animal model for Alport syndrome in humans and progressive chronic kidney disease (CKD). Using mRNA sequencing (mRNA-seq), we have characterized the gene expression profile affecting the progression of XLHN; however, the microRNA (miRN...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Candice P. Chu, Shiguang Liu, Wenping Song, Ethan Y. Xu, Mary B. Nabity
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3328a2122ff94a219f309016a106ffb7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3328a2122ff94a219f309016a106ffb7
record_format	dspace
spelling	oai:doaj.org-article:3328a2122ff94a219f309016a106ffb72021-12-02T16:37:37ZSmall RNA sequencing evaluation of renal microRNA biomarkers in dogs with X-linked hereditary nephropathy10.1038/s41598-021-96870-y2045-2322https://doaj.org/article/3328a2122ff94a219f309016a106ffb72021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96870-yhttps://doaj.org/toc/2045-2322Abstract Dogs with X-linked hereditary nephropathy (XLHN) are an animal model for Alport syndrome in humans and progressive chronic kidney disease (CKD). Using mRNA sequencing (mRNA-seq), we have characterized the gene expression profile affecting the progression of XLHN; however, the microRNA (miRNA, miR) expression remains unknown. With small RNA-seq and quantitative RT-PCR (qRT-PCR), we used 3 small RNA-seq analysis tools (QIAGEN OmicSoft Studio, miRDeep2, and CPSS 2.0) to profile differentially expressed renal miRNAs, top-ranked miRNA target genes, and enriched biological processes and pathways in CKD progression. Twenty-three kidney biopsies were collected from 5 dogs with XLHN and 4 age-matched, unaffected littermates at 3 clinical time points (T1: onset of proteinuria, T2: onset of azotemia, and T3: advanced azotemia). We identified up to 23 differentially expressed miRNAs at each clinical time point. Five miRNAs (miR-21, miR-146b, miR-802, miR-142, miR-147) were consistently upregulated in affected dogs. We identified miR-186 and miR-26b as effective reference miRNAs for qRT-PCR. This study applied small RNA-seq to identify differentially expressed miRNAs that might regulate critical pathways contributing to CKD progression in dogs with XLHN.Candice P. ChuShiguang LiuWenping SongEthan Y. XuMary B. NabityNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Candice P. Chu Shiguang Liu Wenping Song Ethan Y. Xu Mary B. Nabity Small RNA sequencing evaluation of renal microRNA biomarkers in dogs with X-linked hereditary nephropathy
description	Abstract Dogs with X-linked hereditary nephropathy (XLHN) are an animal model for Alport syndrome in humans and progressive chronic kidney disease (CKD). Using mRNA sequencing (mRNA-seq), we have characterized the gene expression profile affecting the progression of XLHN; however, the microRNA (miRNA, miR) expression remains unknown. With small RNA-seq and quantitative RT-PCR (qRT-PCR), we used 3 small RNA-seq analysis tools (QIAGEN OmicSoft Studio, miRDeep2, and CPSS 2.0) to profile differentially expressed renal miRNAs, top-ranked miRNA target genes, and enriched biological processes and pathways in CKD progression. Twenty-three kidney biopsies were collected from 5 dogs with XLHN and 4 age-matched, unaffected littermates at 3 clinical time points (T1: onset of proteinuria, T2: onset of azotemia, and T3: advanced azotemia). We identified up to 23 differentially expressed miRNAs at each clinical time point. Five miRNAs (miR-21, miR-146b, miR-802, miR-142, miR-147) were consistently upregulated in affected dogs. We identified miR-186 and miR-26b as effective reference miRNAs for qRT-PCR. This study applied small RNA-seq to identify differentially expressed miRNAs that might regulate critical pathways contributing to CKD progression in dogs with XLHN.
format	article
author	Candice P. Chu Shiguang Liu Wenping Song Ethan Y. Xu Mary B. Nabity
author_facet	Candice P. Chu Shiguang Liu Wenping Song Ethan Y. Xu Mary B. Nabity
author_sort	Candice P. Chu
title	Small RNA sequencing evaluation of renal microRNA biomarkers in dogs with X-linked hereditary nephropathy
title_short	Small RNA sequencing evaluation of renal microRNA biomarkers in dogs with X-linked hereditary nephropathy
title_full	Small RNA sequencing evaluation of renal microRNA biomarkers in dogs with X-linked hereditary nephropathy
title_fullStr	Small RNA sequencing evaluation of renal microRNA biomarkers in dogs with X-linked hereditary nephropathy
title_full_unstemmed	Small RNA sequencing evaluation of renal microRNA biomarkers in dogs with X-linked hereditary nephropathy
title_sort	small rna sequencing evaluation of renal microrna biomarkers in dogs with x-linked hereditary nephropathy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3328a2122ff94a219f309016a106ffb7
work_keys_str_mv	AT candicepchu smallrnasequencingevaluationofrenalmicrornabiomarkersindogswithxlinkedhereditarynephropathy AT shiguangliu smallrnasequencingevaluationofrenalmicrornabiomarkersindogswithxlinkedhereditarynephropathy AT wenpingsong smallrnasequencingevaluationofrenalmicrornabiomarkersindogswithxlinkedhereditarynephropathy AT ethanyxu smallrnasequencingevaluationofrenalmicrornabiomarkersindogswithxlinkedhereditarynephropathy AT marybnabity smallrnasequencingevaluationofrenalmicrornabiomarkersindogswithxlinkedhereditarynephropathy
_version_	1718383624045199360

Small RNA sequencing evaluation of renal microRNA biomarkers in dogs with X-linked hereditary nephropathy

Ejemplares similares