Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan

Tryptophan metabolism plays a role in the occurrence and development of hepatocellular carcinoma cells. By degrading certain amino acids, tumor growth can be limited while maintaining the body’s normal nutritional requirements. Tryptophan side-chain oxidase (TSO) enzyme can degrade tryptophan, and i...

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Autores principales:	Yang Ai, Ben Wang, Shuai Xiao, Sang Luo, Yefu Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hepatocellular carcinoma tryptophan metabolism enzyme <i>de novo</i> sequencing anticancer drugs Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/3329118251a3459c810d2dd6b6451e08
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spelling	oai:doaj.org-article:3329118251a3459c810d2dd6b6451e082021-11-25T17:56:33ZTryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan10.3390/ijms2222124281422-00671661-6596https://doaj.org/article/3329118251a3459c810d2dd6b6451e082021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12428https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Tryptophan metabolism plays a role in the occurrence and development of hepatocellular carcinoma cells. By degrading certain amino acids, tumor growth can be limited while maintaining the body’s normal nutritional requirements. Tryptophan side-chain oxidase (TSO) enzyme can degrade tryptophan, and its inhibitory effect on hepatocellular carcinoma cells is worthy of further study. To investigate the degradation effect on tryptophan, TSO was isolated and purified from qq Pseudomonas. The reaction products were identified with high performance liquid chromatography (HPLC) and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS). De novo sequencing provided the complete amino acid sequence of TSO. The results of CCK-8, colony formation, transwell, and qPCR confirmed that TSO had inhibitory effects on the proliferation and migration of HCCLM3 (human hepatocarcinoma cell line) and HepG2 cells. The results of flow cytometry confirmed its apoptotic activity. In animal experiments, we found that the tumor-suppressive effect was better in the oncotherapy group than the intraperitoneal injection group. The results of immunohistochemistry also suggested that TSO could inhibit proliferation and promote apoptosis. In conclusion, a specific enzyme that can degrade tryptophan and inhibit the growth of hepatoma cells was authenticated, and its basic information was obtained by extraction/purification and amino acid sequencing.Yang AiBen WangShuai XiaoSang LuoYefu WangMDPI AGarticlehepatocellular carcinomatryptophan metabolismenzyme<i>de novo</i> sequencinganticancer drugsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12428, p 12428 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatocellular carcinoma tryptophan metabolism enzyme <i>de novo</i> sequencing anticancer drugs Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	hepatocellular carcinoma tryptophan metabolism enzyme <i>de novo</i> sequencing anticancer drugs Biology (General) QH301-705.5 Chemistry QD1-999 Yang Ai Ben Wang Shuai Xiao Sang Luo Yefu Wang Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan
description	Tryptophan metabolism plays a role in the occurrence and development of hepatocellular carcinoma cells. By degrading certain amino acids, tumor growth can be limited while maintaining the body’s normal nutritional requirements. Tryptophan side-chain oxidase (TSO) enzyme can degrade tryptophan, and its inhibitory effect on hepatocellular carcinoma cells is worthy of further study. To investigate the degradation effect on tryptophan, TSO was isolated and purified from qq Pseudomonas. The reaction products were identified with high performance liquid chromatography (HPLC) and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS). De novo sequencing provided the complete amino acid sequence of TSO. The results of CCK-8, colony formation, transwell, and qPCR confirmed that TSO had inhibitory effects on the proliferation and migration of HCCLM3 (human hepatocarcinoma cell line) and HepG2 cells. The results of flow cytometry confirmed its apoptotic activity. In animal experiments, we found that the tumor-suppressive effect was better in the oncotherapy group than the intraperitoneal injection group. The results of immunohistochemistry also suggested that TSO could inhibit proliferation and promote apoptosis. In conclusion, a specific enzyme that can degrade tryptophan and inhibit the growth of hepatoma cells was authenticated, and its basic information was obtained by extraction/purification and amino acid sequencing.
format	article
author	Yang Ai Ben Wang Shuai Xiao Sang Luo Yefu Wang
author_facet	Yang Ai Ben Wang Shuai Xiao Sang Luo Yefu Wang
author_sort	Yang Ai
title	Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan
title_short	Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan
title_full	Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan
title_fullStr	Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan
title_full_unstemmed	Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan
title_sort	tryptophan side-chain oxidase enzyme suppresses hepatocellular carcinoma growth through degradation of tryptophan
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3329118251a3459c810d2dd6b6451e08
work_keys_str_mv	AT yangai tryptophansidechainoxidaseenzymesuppresseshepatocellularcarcinomagrowththroughdegradationoftryptophan AT benwang tryptophansidechainoxidaseenzymesuppresseshepatocellularcarcinomagrowththroughdegradationoftryptophan AT shuaixiao tryptophansidechainoxidaseenzymesuppresseshepatocellularcarcinomagrowththroughdegradationoftryptophan AT sangluo tryptophansidechainoxidaseenzymesuppresseshepatocellularcarcinomagrowththroughdegradationoftryptophan AT yefuwang tryptophansidechainoxidaseenzymesuppresseshepatocellularcarcinomagrowththroughdegradationoftryptophan
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Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan

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