Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.

The equilibrium of membrane fusion and fission influences the volume and copy number of organelles. Fusion of yeast vacuoles has been well characterized but their fission and the mechanisms determining vacuole size and abundance remain poorly understood. We therefore attempted to systematically char...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lydie Michaillat, Andreas Mayer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/332a8b11f8764db2a8c8b4e8e193ac02
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:332a8b11f8764db2a8c8b4e8e193ac02
record_format	dspace
spelling	oai:doaj.org-article:332a8b11f8764db2a8c8b4e8e193ac022021-11-18T07:59:02ZIdentification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.1932-620310.1371/journal.pone.0054160https://doaj.org/article/332a8b11f8764db2a8c8b4e8e193ac022013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23383298/?tool=EBIhttps://doaj.org/toc/1932-6203The equilibrium of membrane fusion and fission influences the volume and copy number of organelles. Fusion of yeast vacuoles has been well characterized but their fission and the mechanisms determining vacuole size and abundance remain poorly understood. We therefore attempted to systematically characterize factors necessary for vacuole fission. Here, we present results of an in vivo screening for deficiencies in vacuolar fragmentation activity of an ordered collection deletion mutants, representing 4881 non-essential genes of the yeast Saccharomyces cerevisiae. The screen identified 133 mutants with strong defects in vacuole fragmentation. These comprise numerous known fragmentation factors, such as the Fab1p complex, Tor1p, Sit4p and the V-ATPase, thus validating the approach. The screen identified many novel factors promoting vacuole fragmentation. Among those are 22 open reading frames of unknown function and three conspicuous clusters of proteins with known function. The clusters concern the ESCRT machinery, adaptins, and lipases, which influence the production of diacylglycerol and phosphatidic acid. A common feature of these factors of known function is their capacity to change membrane curvature, suggesting that they might promote vacuole fragmentation via this property.Lydie MichaillatAndreas MayerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e54160 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lydie Michaillat Andreas Mayer Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.
description	The equilibrium of membrane fusion and fission influences the volume and copy number of organelles. Fusion of yeast vacuoles has been well characterized but their fission and the mechanisms determining vacuole size and abundance remain poorly understood. We therefore attempted to systematically characterize factors necessary for vacuole fission. Here, we present results of an in vivo screening for deficiencies in vacuolar fragmentation activity of an ordered collection deletion mutants, representing 4881 non-essential genes of the yeast Saccharomyces cerevisiae. The screen identified 133 mutants with strong defects in vacuole fragmentation. These comprise numerous known fragmentation factors, such as the Fab1p complex, Tor1p, Sit4p and the V-ATPase, thus validating the approach. The screen identified many novel factors promoting vacuole fragmentation. Among those are 22 open reading frames of unknown function and three conspicuous clusters of proteins with known function. The clusters concern the ESCRT machinery, adaptins, and lipases, which influence the production of diacylglycerol and phosphatidic acid. A common feature of these factors of known function is their capacity to change membrane curvature, suggesting that they might promote vacuole fragmentation via this property.
format	article
author	Lydie Michaillat Andreas Mayer
author_facet	Lydie Michaillat Andreas Mayer
author_sort	Lydie Michaillat
title	Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.
title_short	Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.
title_full	Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.
title_fullStr	Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.
title_full_unstemmed	Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.
title_sort	identification of genes affecting vacuole membrane fragmentation in saccharomyces cerevisiae.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/332a8b11f8764db2a8c8b4e8e193ac02
work_keys_str_mv	AT lydiemichaillat identificationofgenesaffectingvacuolemembranefragmentationinsaccharomycescerevisiae AT andreasmayer identificationofgenesaffectingvacuolemembranefragmentationinsaccharomycescerevisiae
_version_	1718422656359858176

Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.

Ejemplares similares