Toxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: In search of relevant pathways of moisture damage-associated health effects

Background: Multiple health effects are associated with moisture damage in buildings. Studies explaining these associations and cell-level mechanisms behind the observed health effects are urgently called for. Objectives: We focused on characterizing gene expression in human airway epithelium after...

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Autores principales:	Maria-Elisa Nordberg, Martin Täubel, Sami Heikkinen, Kaisa Jalkanen, Arto Köliö, Marianne Stranger, Hanna Leppänen, Anne Hyvärinen, Kati Huttunen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Indoor air Health effects Moisture damage Gene expression Particulate matter In vitro Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/332f506f1ff8457e94133e54b120dbef
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spelling	oai:doaj.org-article:332f506f1ff8457e94133e54b120dbef2021-11-28T04:27:33ZToxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: In search of relevant pathways of moisture damage-associated health effects0160-412010.1016/j.envint.2021.106997https://doaj.org/article/332f506f1ff8457e94133e54b120dbef2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S016041202100622Xhttps://doaj.org/toc/0160-4120Background: Multiple health effects are associated with moisture damage in buildings. Studies explaining these associations and cell-level mechanisms behind the observed health effects are urgently called for. Objectives: We focused on characterizing gene expression in human airway epithelium after exposure to indoor air particulate matter (PM) sampled from houses with and without moisture damage, alongside determination of general toxicological markers. Methods: We performed detailed technical building inspections in 25 residential houses and categorized them based on the detection of moisture damages and the probability of occupant exposure. PM sampling was complemented by microbiological and volatile organic compound assessment. We exposed human airway constructs to three dilutions (1:16, 1:8, 1:4) of collected PM from moisture-damaged (index) and non-moisture-damaged (reference) houses and imaged selected constructs with electron microscopy. We analyzed general toxicological markers and the RNA of exposed constructs was sequenced targeting genes associated with toxicological pathways. We did groupwise comparisons between index and reference houses and pairwise comparisons in matched index/reference houses. Results: In groupwise comparison, gene Cytochrome P450 Family 1 Subfamily A Member 1 (CYP1A1) was statistically significantly over-expressed in index houses at all dilutions of collected PM and Nuclear Factor Kappa B Subunit 1 (NFKB1) at dilution 1:4 of collected PM. In pairwise index/reference house comparison, several genes related to multiple toxicological pathways were activated, largest expression differences seen for CYP1A1. However, none of the genes was consistently expressed in all the matched pairs, and general toxicological markers did not differentiate index and reference houses. Discussion: The exposure to PM from index houses activated toxicology -related genes in airway constructs. Differential expression was not consistent among all the index/reference pairs, possibly due to compositional differences of bioactive particles. Our study highlights CYP1A1 and NFKB1 as potential targets in moisture damage -associated cellular responses.Maria-Elisa NordbergMartin TäubelSami HeikkinenKaisa JalkanenArto KöliöMarianne StrangerHanna LeppänenAnne HyvärinenKati HuttunenElsevierarticleIndoor airHealth effectsMoisture damageGene expressionParticulate matterIn vitroEnvironmental sciencesGE1-350ENEnvironment International, Vol 158, Iss , Pp 106997- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Indoor air Health effects Moisture damage Gene expression Particulate matter In vitro Environmental sciences GE1-350
spellingShingle	Indoor air Health effects Moisture damage Gene expression Particulate matter In vitro Environmental sciences GE1-350 Maria-Elisa Nordberg Martin Täubel Sami Heikkinen Kaisa Jalkanen Arto Köliö Marianne Stranger Hanna Leppänen Anne Hyvärinen Kati Huttunen Toxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: In search of relevant pathways of moisture damage-associated health effects
description	Background: Multiple health effects are associated with moisture damage in buildings. Studies explaining these associations and cell-level mechanisms behind the observed health effects are urgently called for. Objectives: We focused on characterizing gene expression in human airway epithelium after exposure to indoor air particulate matter (PM) sampled from houses with and without moisture damage, alongside determination of general toxicological markers. Methods: We performed detailed technical building inspections in 25 residential houses and categorized them based on the detection of moisture damages and the probability of occupant exposure. PM sampling was complemented by microbiological and volatile organic compound assessment. We exposed human airway constructs to three dilutions (1:16, 1:8, 1:4) of collected PM from moisture-damaged (index) and non-moisture-damaged (reference) houses and imaged selected constructs with electron microscopy. We analyzed general toxicological markers and the RNA of exposed constructs was sequenced targeting genes associated with toxicological pathways. We did groupwise comparisons between index and reference houses and pairwise comparisons in matched index/reference houses. Results: In groupwise comparison, gene Cytochrome P450 Family 1 Subfamily A Member 1 (CYP1A1) was statistically significantly over-expressed in index houses at all dilutions of collected PM and Nuclear Factor Kappa B Subunit 1 (NFKB1) at dilution 1:4 of collected PM. In pairwise index/reference house comparison, several genes related to multiple toxicological pathways were activated, largest expression differences seen for CYP1A1. However, none of the genes was consistently expressed in all the matched pairs, and general toxicological markers did not differentiate index and reference houses. Discussion: The exposure to PM from index houses activated toxicology -related genes in airway constructs. Differential expression was not consistent among all the index/reference pairs, possibly due to compositional differences of bioactive particles. Our study highlights CYP1A1 and NFKB1 as potential targets in moisture damage -associated cellular responses.
format	article
author	Maria-Elisa Nordberg Martin Täubel Sami Heikkinen Kaisa Jalkanen Arto Köliö Marianne Stranger Hanna Leppänen Anne Hyvärinen Kati Huttunen
author_facet	Maria-Elisa Nordberg Martin Täubel Sami Heikkinen Kaisa Jalkanen Arto Köliö Marianne Stranger Hanna Leppänen Anne Hyvärinen Kati Huttunen
author_sort	Maria-Elisa Nordberg
title	Toxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: In search of relevant pathways of moisture damage-associated health effects
title_short	Toxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: In search of relevant pathways of moisture damage-associated health effects
title_full	Toxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: In search of relevant pathways of moisture damage-associated health effects
title_fullStr	Toxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: In search of relevant pathways of moisture damage-associated health effects
title_full_unstemmed	Toxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: In search of relevant pathways of moisture damage-associated health effects
title_sort	toxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: in search of relevant pathways of moisture damage-associated health effects
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/332f506f1ff8457e94133e54b120dbef
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Toxicological transcriptome of human airway constructs after exposure to indoor air particulate matter: In search of relevant pathways of moisture damage-associated health effects

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